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Biotechnology Letters

, Volume 27, Issue 8, pp 583–587 | Cite as

Induction method of tyrosine kinase A-mediated cell death in rat pheochromocytoma

  • Jin-Young Ahn
  • Lami Kang
  • Hyun-Jeong Kim
  • Hyejin Kang
  • Sung-Hoon Kim
  • Je-Yong Kim
  • Seung Ki Kim
  • Kyu-Chang Wang
  • Byung Kyu Cho
  • Kwang-Woo Lee
  • Jae-Kyu Roh
  • Manho KimEmail author
Article
  • 47 Downloads

Abstract

Nerve Growth Factor (NGF) is a neurotrophic factor that prevents apoptosis in neuronal progenitor cells. In rat pheochromocytoma (PC12) cells, tyrosine kinase A receptor (TrkA) mediates neurotrophic or protective effects, while p75 neurotrophin receptor (p75NTR) functions as a death receptor. We have determined whether TrkA mediates any cytotoxic effect. Following serum deprivation, TrkA expression increased 2.2-fold and apoptosis began with expression of Bax proapoptotic protein. Application of NGF halved cell viability but this was reversed by K252a, the TrkA inhibitor. These results confirmed the paradoxical cytotoxic effect of neurotrophic NGF via TrkA in PC12 cells following serum deprivation.

Keywords:

apoptosis nerve growth factor rat pheochromocytoma PC12 cell tyrosine kinase A 

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Copyright information

© Springer 2005

Authors and Affiliations

  • Jin-Young Ahn
    • 4
  • Lami Kang
    • 1
  • Hyun-Jeong Kim
    • 3
  • Hyejin Kang
    • 5
  • Sung-Hoon Kim
    • 6
  • Je-Yong Kim
    • 2
  • Seung Ki Kim
    • 2
  • Kyu-Chang Wang
    • 2
  • Byung Kyu Cho
    • 2
  • Kwang-Woo Lee
    • 1
  • Jae-Kyu Roh
    • 1
  • Manho Kim
    • 1
    Email author
  1. 1.Department of Neurology, Clinical Research InstituteSeoul National University HospitalSeoulKorea
  2. 2.Department of Neurosurgery, Clinical Research InstituteSeoul National University HospitalSeoulKorea
  3. 3.Department of Dental Anesthesiology, College of DentistrySeoul National University HospitalSeoulKorea
  4. 4.Department of NeurologyKangnam General Hospital Public CropSeoulKorea
  5. 5.Center for Learning and MemoryMassachusetts Institute of TechnologyCambridgeUSA
  6. 6.Department of NeurologyUniversity of KangwonKorea

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