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Biotechnology Letters

, Volume 27, Issue 7, pp 511–517 | Cite as

Dielectric monitoring of growth and sporulation of Bacillus thuringiensis

  • M.H. Sarrafzadeh
  • L. Belloy
  • G. Esteban
  • J.M. Navarro
  • C. Ghommidh
Article

Abstract

On-line permittivity and optical density measurements have been used to monitor biomass concentration and sporulation status during growth of a spore-forming bacterium, Bacillus thuringiensis, in fed-batch culture. The correlation between permittivity, optical density and other observations showed three distinct phases of growth: growth itself, transition and sporulation. The permittivity variations during the transition and sporulation phases could be related to the sporulation development: the evolution pattern of the ratio of optical density to permittivity was representative of the culture state, and during the sporulation phase, a permittivity index could be build to measure the extend of spore liberation.

Keywords

biomass measurement capacitance permittivity spore-forming bacteria segregated model 

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References

  1. Liu, W, Bajpai, R, Bihari, V 1994High-density cultivation of sporeformersAnn. N.Y. Acad. Sci.721310325PubMedGoogle Scholar
  2. Losick, R, Youngman, P, Piggot, PJ 1986Genetics of endospore formation in Bacillus subtilisAnn. Rev. Genet.20625669CrossRefPubMedGoogle Scholar
  3. Piggot, PJ, Bylund, JE, Higgins, ML 1994Morphogenesis and gene expression during sporulationPiggot, PJMoran, CPYoungman, P eds. Regulation of Bacterial Differentiation.American Society for MicrobiologyWashington, DC113137Google Scholar
  4. Schwan, HP 1957Electrical properties of tissue and cell suspensionsAdv. Biol. Med. Phys.5147209PubMedGoogle Scholar
  5. Setlow, P 1994Mechanisms which contribute to the long term survival of spores of Bacillus speciesJ. Appl. Bacteriol.7649S69SGoogle Scholar
  6. Stragier, P, Losick, R 1996Molecular genetics of sporulation in Bacillus subtilisAnn. Rev. Genet.30297341CrossRefPubMedGoogle Scholar
  7. Yardley, JE, Kell, DB, Barrett, J, Davey, CL 2000On-line, real-time measurements of cellular biomass using dielectric spectroscopyBiotechnol. Gen. Eng. Rev.17335Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • M.H. Sarrafzadeh
    • 1
    • 2
  • L. Belloy
    • 1
  • G. Esteban
    • 3
  • J.M. Navarro
    • 1
  • C. Ghommidh
    • 1
  1. 1.UMR Ingénierie de la Réaction Biologique – BioproductionsUniversité Montpellier IIMontpellier
  2. 2.Engineering DepartmentPersian Gulf UniversityBoushehrIran
  3. 3.FOGALE NanotechNîmesFrance

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