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Biotechnology Letters

, Volume 27, Issue 7, pp 499–503 | Cite as

Rational strategy for the production of new crude lipases from Candida rugosa

  • Pablo Domínguez de María
  • José M. Sánchez-MonteroEmail author
  • Andrés R. Alcántara
  • Francisco Valero
  • José V. Sinisterra
Article

Abstract

Candida rugosa was cultured using different inducers (oleic acid, olive oil, sunflower oil, n-dodecanol and glycerol) as the only carbon source in batch conditions, as well as in several fed-batch fermentations (oleic acid as inducer) at variable feed rate conditions. The N-terminal analysis of each crude lipase revealed that, while the isoenzymes Lip2 and Lip3 are always secreted (at different proportions depending on the inducer), Lip1 was produced only using n-dodecanol (batch conditions) or oleic acid (fed-batch at high feed rate). The nature of the inducer controls the isoenzyme percentage; when this is fixed, as well as the feed rate in fed-batch fermentation, the isoenzymatic profile remained unaltered and the samples differed only in the activity of the lipases, as determined by heptyl oleate synthesis.

Keywords

biotransformations Candida rugosa lipases isoenzymes 

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References

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Copyright information

© Springer 2005

Authors and Affiliations

  • Pablo Domínguez de María
    • 1
  • José M. Sánchez-Montero
    • 1
    Email author
  • Andrés R. Alcántara
    • 1
  • Francisco Valero
    • 2
  • José V. Sinisterra
    • 1
  1. 1.Grupo de Biotransformaciones, Departamento de Química Orgánica y Farmacéutica, Facultad de FarmaciaUniversidad Complutense MadridPlaza de Ramón y Cajal, s/n. Ciudad UniversitariaSpain
  2. 2.Departament d’Enginyeria Química, Escola Tècnica Superior d’EnginyeriaUniversitat Autònoma de BarcelonaBarcelonaSpain

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