Biotechnology Letters

, Volume 27, Issue 5, pp 317–322

In vitro study of alginate–chitosan microcapsules: an alternative to liver cell transplants for the treatment of liver failure

  • Tasima Haque
  • Hongmei Chen
  • Wei Ouyang
  • Christopher Martoni
  • Bisi Lawuyi
  • Aleksandra Malgorzata Urbanska
  • Satya Prakash
Article

DOI: 10.1007/s10529-005-0687-3

Cite this article as:
Haque, T., Chen, H., Ouyang, W. et al. Biotechnol Lett (2005) 27: 317. doi:10.1007/s10529-005-0687-3

Abstract

The application of alginate–chitosan (AC) microcapsules to liver cell transplantation has not been previously investigated. In the current in vitro study, we have investigated the potential of AC microcapsules for the encapsulation of liver cells and show that the AC membrane supports the survival, proliferation and protein secretion by entrapped hepatocytes. The AC membrane provides cell immuno-isolation and has the potential for cell cryopreservation. The AC microcapsule has several advantages compared to more widely used alginate–poly-L-lysine (APA) microcapsules for the application of cell therapy.

Keywords

alginate cell viability chitosan hepatocytes microencapsulation 

Copyright information

© Springer 2005

Authors and Affiliations

  • Tasima Haque
    • 1
  • Hongmei Chen
    • 1
  • Wei Ouyang
    • 1
  • Christopher Martoni
    • 1
  • Bisi Lawuyi
    • 1
  • Aleksandra Malgorzata Urbanska
    • 1
  • Satya Prakash
    • 1
  1. 1.Biomedical Technology and Cell Therapy Research Laboratory, Departments of Biomedical Engineering and Artificial Cells and Organs Research Center, Faculty of MedicineMcGill UniversityMontrealCanada

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