Biotechnology Letters

, Volume 27, Issue 5, pp 297–303 | Cite as

Enhanced radical scavenging activity of genetically modified Arabidopsis seeds

  • Takayuki Tohge
  • Kyoko Matsui
  • Masaru Ohme-Takagi
  • Mami Yamazaki
  • Kazuki SaitoEmail author


The proanthocyanidin (PA) content was increased in seeds of pap1-D mutant of Arabidopsis thaliana, in which the expression of endogenous PAP1 gene encoding a Myb-like transcription factor was induced by activation-tagging with enhancer sequences derived from cauliflower mosaic virus 35S promoter. In contrast, the PA contents decreased in seeds of transgenic plants transformed with a PAP1 cDNA or with a PAP1 chimeric repressor, although the amount of soluble anthocyanins increased in seeds of transgenic plants over-expressing PAP1cDNA. The enhanced radical scavenging activity was observed only in the seed extracts of pap1-D mutant, indicating that PAs are primarily responsible for radical scavenging activity in seeds. The present study suggests the feasibility of engineering a transcription factor of flavonoid biosynthesis for health beneficial plant seeds.


anthocyanin arabidopsis thaliana MYB-like transcription factor proanthocyanidin radical scavenging 


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  1. Abrahams, S, Tanner, GJ, Larkin, PJ, Ashton, AR 2002Identification and biochemical characterization of mutants in the proanthocyanidin pathway in ArabidopsisPlant Physiol.130561576Google Scholar
  2. Ames, BN 1983Dietary carcinogens and anticarcinogens. (oxygen radicals and degenerative diseases)Science22112561264Google Scholar
  3. Bagchi, D, Bagchi, M, Stohs, SJ, Das, DK, Ray, SD, Kuszynski, CA, Joshi, SS, Pruess, HG 2000Free radicals and grape seed proanthocyanidin extract: importance in human health and disease preventionToxicology148187197Google Scholar
  4. Beninger, CW, Hosfield, GL 2003Antioxidant activity of extracts, condensed tannin fractions, and pure flavonoids from Phaseolus vulgaris L. seed coat color genotypesJ. Agric. Food Chem.5178797883Google Scholar
  5. Blois, MS 1958Antioxidant determinations by the use of a stable free radicalNature461711991200Google Scholar
  6. Bloor, SJ, Abrahams, S 2002The structure of the major anthocyanin in Arabidopsis thalianaPhytochemistry59343346Google Scholar
  7. Borevitz, JO, Xia, Y, Blount, J, Dixon, RA, Lamb, , C,  2000Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesisPlant Cell1223832393Google Scholar
  8. Cos, P, De Bruyne, T, Hermans, N, Apers, S, Berghe, DV, Vlietinck, AJ 2004Proanthocyanidins in health care: current and new trendsCurr. Med. Chem.1113451359Google Scholar
  9. Dalzell, SA, Kerven, GL 1998A rapid method for the measurement of Leucaenaspp. proanthocyanidins by the proanthocyanidin (BuOH/HCl) assayJ. Sci. Food Agric.78405416Google Scholar
  10. Gabetta, B, Fuzzati, N, Griffini, A, Lolla, E, Pace, R, Ruffilli, T, Peterlongo, F 2000Characterization of proanthocyanidins from grape seedsFitoterapia71162175Google Scholar
  11. Hiratsu, K, Matsui, K, Koyama, T, Ohme-Takagi, M 2003Dominant repression of target genes by chimeric repressors that include the EAR motif, a repression domain, in ArabidopsisPlant J.34733739Google Scholar
  12. Hou, DX 2003Potential mechanisms of cancer chemoprevention by anthocyaninsCurr. Mol. Med.3149159Google Scholar
  13. Jones, P, Messner, B, Nakajima, J, Schäffner, AR, Saito, , K,  2003UGT73C6 and UGT78D1, glycosyltransferases involved in flavonol glycoside biosynthesis in Arabidopsis thalianaJ. Biol. Chem.454391043918Google Scholar
  14. Lin, LC, Kuo, YC, Chou, CJ 2002Immunomodulatory proanthocyanidins from Ecdysanthera utilisJ Nat Prod.65505508Google Scholar
  15. Marles, MAS, Ray, H, Gruber, MY 2003New perspectives on proanthocyanidin biochemistry and molecular regulationPhytochemistry64367383Google Scholar
  16. Matsui, K, Tanaka, H, Ohme-takagi, M 2004Suppression of the biosynthesis of proanthocyanidin in Arabidopsis by a chimeric PAP1 repressorPlant Biotech. J.2487493Google Scholar
  17. Nakajima, J, Tanaka, I, Seo, S, Yamazaki, M, Saito, K 2004LC/PDA/ESI-MS profiling and radical scavenging activity of anthocyanins in various berriesJ. Biomed. Biotech.2004241247Google Scholar
  18. Niggeweg, R, Michael, AJ, Martin, C 2004Engineering plants with increased levels of the antioxidant chlorogenic acidNature Biotech.22746754Google Scholar
  19. Plumb, GW, De Pascual-Teresa, S, Santos-Buelga, C, Cheynier, V, Williamson, G 1998Antioxidant properties of catechins and proanthocyanidins: effect of polymerisation, galloylation and glycosylationFree Radic. Res.129351358Google Scholar
  20. Ray, H, Yu, M, Auser, P, Blahut-Beatty, L, McKersie, B, Bowley, S, Westcott, N, Coulman, B, Llyod, A, Gruber, M 2003Expression of anthocyanins and proanthocyanidins after transformation of Alfalfa with Maize LcPlant Physiol.13214481463Google Scholar
  21. Takahata, Y, Ohnishi-Kameyama, M, Furuta, S, Takahashi, M, Suda, I 2001Highly polymerized proanthocyanidins in brown soybean seed coat with a high radical-scavenging activityJ. Agric. Food Chem.4958435847Google Scholar
  22. Tohge T, Nishiyama Y, Yokota-Hirai M, Yano M, Nakajima J, Awazuhara M, Inoue E, Takahashi H, Goodenowe DB, Kitayama M, Noji M, Yamazaki M, Saito K. (2005) Functional genomics by integrated analysis of metabolome and transcriptome ofArabidopsis plants overexpressing a MYB transcription factor.Plant J. (in press)Google Scholar
  23. Veit, M, Pauli, GF 1999Major flavonoids from Arabidopsis thaliana leavesJ. Nat. Prod.6213011303Google Scholar
  24. Winkel-Shirley, B 2001It takes a garden. How work on diverse plant species has contributed to an understanding of flavonoid metabolismPlant Physiol.12713991404Google Scholar
  25. Yamazaki, M, Nakajima, J, Yamanashi, M, Sugiyama, M, Makita, Y, Springob, K, Awazuhara, M, Saito, K 2003Metabolomics and differential gene expression in anthocyanin chemo-varietal forms of Perilla frutescensPhytochemistry62987995Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Takayuki Tohge
    • 1
  • Kyoko Matsui
    • 2
    • 3
  • Masaru Ohme-Takagi
    • 2
    • 3
  • Mami Yamazaki
    • 1
  • Kazuki Saito
    • 1
    • 3
    Email author
  1. 1.Department of Molecular Biology and BiotechnologyGraduate School of Pharmaceutical Sciences, Chiba UniversityInage-kuJapan
  2. 2.Gene Function Research CenterNational Institute of Advanced Industrial Science and Technology (AIST)TsukubaJapan
  3. 3.CRESTJapan Science and Technology AgencyKawaguchiJapan

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