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Biotechnology Letters

, Volume 27, Issue 5, pp 297–303 | Cite as

Enhanced radical scavenging activity of genetically modified Arabidopsis seeds

  • Takayuki Tohge
  • Kyoko Matsui
  • Masaru Ohme-Takagi
  • Mami Yamazaki
  • Kazuki SaitoEmail author
Article

Abstract

The proanthocyanidin (PA) content was increased in seeds of pap1-D mutant of Arabidopsis thaliana, in which the expression of endogenous PAP1 gene encoding a Myb-like transcription factor was induced by activation-tagging with enhancer sequences derived from cauliflower mosaic virus 35S promoter. In contrast, the PA contents decreased in seeds of transgenic plants transformed with a PAP1 cDNA or with a PAP1 chimeric repressor, although the amount of soluble anthocyanins increased in seeds of transgenic plants over-expressing PAP1cDNA. The enhanced radical scavenging activity was observed only in the seed extracts of pap1-D mutant, indicating that PAs are primarily responsible for radical scavenging activity in seeds. The present study suggests the feasibility of engineering a transcription factor of flavonoid biosynthesis for health beneficial plant seeds.

Keywords

anthocyanin arabidopsis thaliana MYB-like transcription factor proanthocyanidin radical scavenging 

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Copyright information

© Springer 2005

Authors and Affiliations

  • Takayuki Tohge
    • 1
  • Kyoko Matsui
    • 2
    • 3
  • Masaru Ohme-Takagi
    • 2
    • 3
  • Mami Yamazaki
    • 1
  • Kazuki Saito
    • 1
    • 3
    Email author
  1. 1.Department of Molecular Biology and BiotechnologyGraduate School of Pharmaceutical Sciences, Chiba UniversityInage-kuJapan
  2. 2.Gene Function Research CenterNational Institute of Advanced Industrial Science and Technology (AIST)TsukubaJapan
  3. 3.CRESTJapan Science and Technology AgencyKawaguchiJapan

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