Biotechnology Letters

, Volume 27, Issue 4, pp 239–242 | Cite as

Thermostable cellulases from Streptomycessp.: scale-up production in a 50-l fermenter

  • Hung-Der JangEmail author
  • Ku-Shang Chang


Thermostable cellulase was produced by Streptomyces sp. T3-1 grown in a 50-l fermenter. Maximum cellulase activity was attained on the fourth day when agitation speeds and aeration rates were controlled at 300 rpm and 0.75 vvm, respectively. Maximum enzyme activities were: 148 IU CMCase ml−1, 45 IU Avicelase ml−1, and 137 IU β-glucosidase ml−1 with productivity of 326 IU l−1 h−1, which were 10--32% higher than the values obtained in shake-flask cultures


aeration rate agitation speed cellulase fermenter Streptomyces 


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  1. Bhat, MK, Bhat, S 1997Cellulose degrading enzymes and their potential industrial applicationsBiotechnol. Adv.15583620Google Scholar
  2. Fernandez-Abalos, JM, Sanchez, P, Coll, PM, Villanueva, JR, Perez, P, Santamaria, RI 1992Cloning and nucleotide sequence of celA1 and endo-β-1, 4-glucanase-encoding gene from Streptomyces halstedii JM8J. Bacteriol.17463686376Google Scholar
  3. Ghose, TK 1987Measurement of cellulase activitiesPure Appl. Chem.59257268Google Scholar
  4. Haki, GD, Rakshit, GD 2003Developments in industrially important thermostable enzymes: a reviewBioresour. Technol.891734Google Scholar
  5. Hopwood, DA, Bibb, MJ, Chater, KF, Bruton, CJ, Kieser, HM, Lydiate, DJ, Smith, CP, Ward, JM 1985Genetic Manipulation of Streptomyces: A Laboratory ManualThe John Innes FoundationNorwich, UKGoogle Scholar
  6. Jang, HD, Chen, KS 2003Production and characterization of thermostable cellulases from Streptomyces transformant T3-1World J. Microbiol. Biotechnol.19263268Google Scholar
  7. Mandels, M, Reese, ET 1964Fungal cellulase and the microbial decomposition of cellulosic fabricDev. Ind. Microbiol.5520Google Scholar
  8. Milner, JA, Martin, DJ, Smith, A 1996Oxygen transfer conditions in the production of alpha-amylase by Bacillus amyloliquefaciensEnzyme Microb. Technol.1850752Google Scholar
  9. Niehaus, F, Bertoldo, C, Kahler, M, Antranikian, G 1999Extremophiles as a source of novel enzymes for industrial applicationsAppl. Microbiol. Biotechnol.51711729Google Scholar
  10. Schrempf, H, Walter, S 1995The cellulolytic system of Streptomyces reticuliInt. J. Biol. Macromol.17353355Google Scholar
  11. Tuncer, M, Ball, AS, Rob, A, Wilson, MT 1999Optimization of extracellular lignocellulolytic enzyme production by a thermophilic actinomycetes Thermomonospora fusca BD25Enzyme Microb. Technol.253847Google Scholar
  12. Wittmann, S, Shareck, F, Kluepfel, D, Morosol, R 1994Purification and characterization of the CelB endoglucanase from Streptomyces lividans 66 and DNA sequence of the encoding geneAppl. Environ. Microbiol.6017011703Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  1. 1.Department of Food ScienceYuanpei University of Science and TechnologyHsinchuTaiwan

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