Biotechnology Letters

, Volume 27, Issue 4, pp 239–242 | Cite as

Thermostable cellulases from Streptomycessp.: scale-up production in a 50-l fermenter

Article

Abstract

Thermostable cellulase was produced by Streptomyces sp. T3-1 grown in a 50-l fermenter. Maximum cellulase activity was attained on the fourth day when agitation speeds and aeration rates were controlled at 300 rpm and 0.75 vvm, respectively. Maximum enzyme activities were: 148 IU CMCase ml−1, 45 IU Avicelase ml−1, and 137 IU β-glucosidase ml−1 with productivity of 326 IU l−1 h−1, which were 10--32% higher than the values obtained in shake-flask cultures

Keywords

aeration rate agitation speed cellulase fermenter Streptomyces 

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Copyright information

© Springer 2005

Authors and Affiliations

  1. 1.Department of Food ScienceYuanpei University of Science and TechnologyHsinchuTaiwan

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