Genome-Wide Identification and Comparative Expression Profile Analysis of the Long-Chain Acyl-CoA synthetase (LACS) Gene Family in Two Different Oil Content Cultivars of Brassica napus
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Long-chain acyl-CoA synthetase (LACS) is one of the key enzymes involved in fatty acid metabolism, including phospholipid biosynthesis, triacylglycerol (TAG) biosynthesis, and fatty acid β-oxidation in plants. However, the characterization of LACSs family in seed oil biosynthesis of Brassica napus (B. napus) remains unknown. In the present study, we performed a comprehensive genome-wide analysis of this gene family in B. napus, and 34 B. napus LACS genes (BnaLACSs) were identified. Phylogenetic analysis classified the BnaLACS proteins into four groups (A, B, C, and D), which were supported by highly conserved gene structures and consensus motifs. RNA-Sequencing (RNA-Seq) and qRT-PCR combined analysis revealed that 18 BnaLACSs (BnaLACS1-2, 1–3, 1–4, 1–9, 1–10, 2–1, 2–2, 4–1, 4–2, 6–1, 6–2, 6–4, 7–1, 7–2, 8–1, 8–2, 9–3, and 9–4) were highly expressed in developmental seeds. Comparative expression analysis between extremely high oil content (P1-HO) and low oil content (P2-LO) B. napus cultivars revealed that BnaLACS6-4, BnaLACS9-3, and BnaLACS9-4 may be involved in fatty acid synthesis in chloroplast, and BnaLACS1-10 and 4–1 may play a vital role in lipid biosynthesis in B. napus, which is important for further seed oil accumulation in oilseed rape. The present study provides important information for functional characterization of BnaLACSs in seed oil metabolism in B. napus.
KeywordBrassica napus. Long-chain acyl-CoA synthetases Phylogenetic analysis RNA-Seq qRT-PCR Expression analysis
This study was funded by National Key R & D Program of China (2018YFD0200903; 2018YFD0100504), the “111″ Project (B12006), Modern Agro-industry Technology Research System (CARS-13), the National Natural Science Foundation of China (31400063; 31571701, U1302266), Chongqing Minsheng Technology Funds (cstc2016shms-ztzx80020), and Fundamental Research Funds for the Central Universities (XDJK2017B030). We extend our thanks to the reviewers for their careful reading and helpful comments on this manuscript.
J.N.L. and N.N.L. conceived and designed the experiments. Z.C.X. performed the experiments. Z.C.X., S. F.W., J.J.S., L.Y. Z., C.Z., H.Y., H.Y.Z., B.Y., L.J.W., H.D., C.M.Q, and K.L. analyzed the data. Z.C.X. and N.N.L. wrote the paper. All authors reviewed the manuscript.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
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