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Biochemical Genetics

, Volume 57, Issue 6, pp 781–800 | Cite as

Genome-Wide Identification and Comparative Expression Profile Analysis of the Long-Chain Acyl-CoA synthetase (LACS) Gene Family in Two Different Oil Content Cultivars of Brassica napus

  • Zhongchun Xiao
  • Nannan LiEmail author
  • Shufeng Wang
  • Juanjuan Sun
  • Liyuan Zhang
  • Chao Zhang
  • Hong Yang
  • Huiyan Zhao
  • Bo Yang
  • Lijuan Wei
  • Hai Du
  • Cunmin Qu
  • Kun Lu
  • Jiana LiEmail author
Original Article
  • 228 Downloads

Abstract

Long-chain acyl-CoA synthetase (LACS) is one of the key enzymes involved in fatty acid metabolism, including phospholipid biosynthesis, triacylglycerol (TAG) biosynthesis, and fatty acid β-oxidation in plants. However, the characterization of LACSs family in seed oil biosynthesis of Brassica napus (B. napus) remains unknown. In the present study, we performed a comprehensive genome-wide analysis of this gene family in B. napus, and 34 B. napus LACS genes (BnaLACSs) were identified. Phylogenetic analysis classified the BnaLACS proteins into four groups (A, B, C, and D), which were supported by highly conserved gene structures and consensus motifs. RNA-Sequencing (RNA-Seq) and qRT-PCR combined analysis revealed that 18 BnaLACSs (BnaLACS1-2, 1–3, 1–4, 1–9, 1–10, 2–1, 2–2, 4–1, 4–2, 6–1, 6–2, 6–4, 7–1, 7–2, 8–1, 8–2, 9–3, and 9–4) were highly expressed in developmental seeds. Comparative expression analysis between extremely high oil content (P1-HO) and low oil content (P2-LO) B. napus cultivars revealed that BnaLACS6-4, BnaLACS9-3, and BnaLACS9-4 may be involved in fatty acid synthesis in chloroplast, and BnaLACS1-10 and 4–1 may play a vital role in lipid biosynthesis in B. napus, which is important for further seed oil accumulation in oilseed rape. The present study provides important information for functional characterization of BnaLACSs in seed oil metabolism in B. napus.

Keyword

Brassica napus. Long-chain acyl-CoA synthetases Phylogenetic analysis RNA-Seq qRT-PCR Expression analysis 

Notes

Acknowledgements

This study was funded by National Key R & D Program of China (2018YFD0200903; 2018YFD0100504), the “111″ Project (B12006), Modern Agro-industry Technology Research System (CARS-13), the National Natural Science Foundation of China (31400063; 31571701, U1302266), Chongqing Minsheng Technology Funds (cstc2016shms-ztzx80020), and Fundamental Research Funds for the Central Universities (XDJK2017B030). We extend our thanks to the reviewers for their careful reading and helpful comments on this manuscript.

Author Contributions

J.N.L. and N.N.L. conceived and designed the experiments. Z.C.X. performed the experiments. Z.C.X., S. F.W., J.J.S., L.Y. Z., C.Z., H.Y., H.Y.Z., B.Y., L.J.W., H.D., C.M.Q, and K.L. analyzed the data. Z.C.X. and N.N.L. wrote the paper. All authors reviewed the manuscript.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10528_2019_9921_MOESM1_ESM.tif (981 kb)
Chromosomal locations of the BnaLACS genes in the B. napus genome. The distribution of each BnaLACS gene was mapped according to the B. napus genome. The chromosome numbers are indicated at the top of each chromosome. The gene names are on the right side of each chromosome, according to the physical location (left side) of each BnaLACS gene. Supplementary file1 (XLSX 13 kb)
10528_2019_9921_MOESM2_ESM.tif (5.8 mb)
3D models of identified LACSs proteins. The tertiary structures were generated by online SWISS-MODEL Workspace. Supplementary file2 (XLSX 13 kb)
10528_2019_9921_MOESM3_ESM.xlsx (14 kb)
Supplementary file3 (XLSX 13 kb)
10528_2019_9921_MOESM4_ESM.xlsx (12 kb)
Supplementary file4 (XLSX 11 kb)
10528_2019_9921_MOESM5_ESM.xlsx (26 kb)
Supplementary file5 (XLSX 26 kb)
10528_2019_9921_MOESM6_ESM.xlsx (12 kb)
Supplementary file6 (XLSX 11 kb)
10528_2019_9921_MOESM7_ESM.xlsx (12 kb)
Supplementary file7 (XLSX 11 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Zhongchun Xiao
    • 1
    • 3
  • Nannan Li
    • 2
    • 3
    Email author
  • Shufeng Wang
    • 2
  • Juanjuan Sun
    • 2
  • Liyuan Zhang
    • 1
    • 3
  • Chao Zhang
    • 1
    • 3
  • Hong Yang
    • 1
    • 3
  • Huiyan Zhao
    • 1
    • 3
  • Bo Yang
    • 1
    • 3
  • Lijuan Wei
    • 1
    • 3
  • Hai Du
    • 1
    • 3
  • Cunmin Qu
    • 1
    • 3
  • Kun Lu
    • 1
    • 3
  • Jiana Li
    • 1
    • 3
    Email author
  1. 1.Chongqing Engineering Research Center for Rapeseed, College of Agronomy and BiotechnologySouthwest UniversityChongqingChina
  2. 2.Research Center of Bioenergy and Bioremediation, College of Resources and EnvironmentSouthwest UniversityChongqingChina
  3. 3.Academy of Agricultural SciencesSouthwest UniversityChongqingChina

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