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IGF2 is Deregulated During the Development of Uterine Cervical Carcinoma in Indian Patients

  • Anirban Roychowdhury
  • Sudip Samadder
  • Dipanjana Indra Mazumder
  • Pijush Das
  • Mukta Basu
  • Ranajit Mondal
  • Anup Roy
  • Susanta Roychoudhury
  • Chinmay Kumar PandaEmail author
Original Article
  • 28 Downloads

Abstract

Uterine cervical carcinoma (CACX) is one of the leading causes of deaths in Indian women. Chromosomal alterations including 11p15.5 locus were reported in CACX. Consequently, we strived for the first time to understand the molecular status of the candidate gene Insulin-like growth factor 2, IGF2 (11p15.5) in Indian CACX patients (n = 128). DNA copy number (CN) analysis using CGH-SNP analysis showed no genetic alteration and it was further validated by comparison with publicly available CN datasets. But promoter hypo-methylation during the progression of CACX was observed and also found to be concordant with publicly available DNA methylation datasets. Interestingly, we found diverse expression of IGF2 transcript in both normal cervical epithelium (NCE) and CACX tumors. Similar heterogeneous expression pattern was seen in publicly available expression datasets as well. Finally, protein expression analysis in NCE showed concordance with transcript expression but tumors showed frequent low expression. Log-rank test showed a difference (p-value = 0.057) in overall survival between cases with and without alteration for IGF2 in Indian CACX patients. Collectively, our study proposes that regulation of IGF2 expression in NCE appeared to be multifaceted and deregulation during the development of CACX resulted in the differential expression.

Keywords

Insulin-like growth factor 2 (IGF2) Cervical cancer (CACX) Normal cervical epithelium (NCE) Gene regulation Methylation-specific PCR (MSP) 

Notes

Acknowledgement

The authors thank the Director, Chittaranjan National Cancer Institute, Kolkata, India. This work was supported by UGC-NET/JRF grant [Sr No. 2121130723] to Mr S. Samadder from University Grants Commission (UGC), UGC-NET Fellowship grant (Sr. No. 2061430780, Ref No.: 22/06/2014(i)EU-V) to Ms M. Basu and grant from Council of Scientific & Industrial Research (CSIR), Government of India [No. 60(0111)/14/EMR-II of dt 03/11/2014] to Dr. C. K. Panda.

Compliance with Ethical Standards

Conflict of interest

The authors have no conflict of interest to declare.

Supplementary material

10528_2019_9917_MOESM1_ESM.tif (1.8 mb)
Supplementary file1 (TIF 1855 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Anirban Roychowdhury
    • 1
  • Sudip Samadder
    • 1
  • Dipanjana Indra Mazumder
    • 2
  • Pijush Das
    • 3
  • Mukta Basu
    • 1
  • Ranajit Mondal
    • 4
  • Anup Roy
    • 5
  • Susanta Roychoudhury
    • 6
  • Chinmay Kumar Panda
    • 1
    Email author
  1. 1.Department of Oncogene RegulationChittaranjan National Cancer InstituteKolkataIndia
  2. 2.Department of ZoologySiliguri CollegeDarjeelingIndia
  3. 3.Structural Biology and Bioinformatics DivisionCSIR-Indian Institute of Chemical BiologyKolkataIndia
  4. 4.Department of Gynecology OncologyChittaranjan National Cancer InstituteKolkataIndia
  5. 5.Department of PathologyNil Ratan Sircar Medical College and HospitalKolkataIndia
  6. 6.Saroj Gupta Cancer Centre & Research InstituteKolkataIndia

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