Biochemical Genetics

, Volume 55, Issue 2, pp 158–167 | Cite as

Association of STAT5A Gene Variants with Milk Production Traits in Agerolese Cattle

  • Maria SelvaggiEmail author
  • Sara Albarella
  • Cataldo Dario
  • Vincenzo Peretti
  • Francesca Ciotola
Original Article


Two polymorphisms at STAT5A gene were investigated in a sample of Agerolese cows. The aims of the present study were to estimate the allele and genotype frequencies and to investigate the relationship among genotypes and milk production traits. Milk production traits were analyzed for each animal in the first, second, third, and fourth lactation. No genetic variability was found at STAT5A/AvaI locus. At STAT5A/MslI locus, the frequencies of T and C alleles were 0.875 and 0.125, respectively. Significant differences between genotypes were found: TT cow produced a milk with a higher content of fat and protein when compared with TC.


STAT5A Gene polymorphisms Agerolese cattle breed Milk yield Milk quality 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. AIA (2014) Quadro economico e Relazione attività 2013.
  2. Antoniou E, Hirts BJ, Grosz M, Skidmorec J (1999) A single strand conformation polymorphism in the bovine gene STAT5A. Anim Genet 30:232CrossRefPubMedGoogle Scholar
  3. Argetsinger LS, Carter-Su C (1996) Growth hormone signalling mechanisms: involvement of the tyrosine kinase JAK2. Horm Res 45:22–24CrossRefPubMedGoogle Scholar
  4. Boucheron C, Dumon S, Santos SC, Moriggl R, Hennighausen L, Gisselbrecht S, Gouilleux F (1998) A single amino acid in the DNA binding regions of STAT5A and STAT5B confers distinct DNA binding specificities. J Biol Chem 273:33936–33941CrossRefPubMedGoogle Scholar
  5. Brym P, Kamińki S, Ruść A (2004) A New SSCP polymorphism within bovine STAT5A gene and its associations with milk performance traits in Black-and-White and Jersey cattle. J Appl Genet 45:445–452PubMedGoogle Scholar
  6. Dario C, Selvaggi M (2011) Study on the STAT5A/AvaI polymorphism in Jersey cows and association with milk production traits. Mol Biol Rep 38:5387–5392CrossRefPubMedGoogle Scholar
  7. Dario C, Dario M, Ciotola F, Peretti V, Carnicella D, Selvaggi M (2009a) Analysis of STAT5A/AvaI gene polymorphism in four Italian cattle breeds. Biochem Genet 47:671–679CrossRefPubMedGoogle Scholar
  8. Dario C, Selvaggi M, Carnicella D, Bufano G (2009b) STAT5A/AvaI polymorphism in Podolica bulls and its effect on growth performance traits. Livest Sci 123:83–87CrossRefGoogle Scholar
  9. Darnell JE, Kerr IM, Stark GR (1994) JAK-STAT pathways and transcriptional activation in response to IFNs and other extracellular signalling proteins. Science 264:1415–1421CrossRefPubMedGoogle Scholar
  10. Falconer DS, Mackay TFC (1996) Introduction to quantitative genetics, 4th edn. Longman Group Ltd, EssexGoogle Scholar
  11. Felius M (1995) Cattle breeds: an encyclopedia. Misset, DoetinchemGoogle Scholar
  12. Flisikowski K, Zwierzchowski L (2003) Polymerase chain reaction-heteroduplex (PCR-HD) polymorphism within the bovine STAT5A gene. J Appl Genet 44:185–189PubMedGoogle Scholar
  13. Flisikowski K, Oprzdek J, Dymnicki E, Zwierzchowski L (2003a) New polymorphism in bovine STAT5A gene and its association with meat production traits in beef cattle. Anim Sci Pap Rep 21:147–157Google Scholar
  14. Flisikowski K, Szymanowska M, Zwierzchowski L (2003b) The DNA binding capacity of genetic variants of the bovine STAT5A transcription factor. Cell Mol Biol Lett 8:831–840PubMedGoogle Scholar
  15. Flisikowski K, Starzynski RR, Zwierzchowski L (2004a) Promoter variant dependent expression of the STAT5A gene in bovine liver. Biochim Biophys Acta 1679:195–199CrossRefPubMedGoogle Scholar
  16. Flisikowski K, Strzałkowska N, Słoniewski K, Krzyzewki J, Zwierzchowski L (2004b) Association of a sequence nucleotide polymorphism in exon 16 of the STAT5A gene with milk production traits in Polish Black-and- White (Polish Friesian) cows. Anim Sci Pap Rep 22:515–522Google Scholar
  17. Herrington J, Smit L, Schwartz J, Carter-Su C (2000) The role of STAT proteins in GH signalling. Oncogene 19:2585–2597CrossRefPubMedGoogle Scholar
  18. Khatib H, Monson RL, Schutzkus V, Kohl DM, Rosa GJ, Rutledge JJ (2008) Mutations in the STAT5A gene are associated with embryonic survival and milk composition in cattle. J Dairy Sci 91:784–793CrossRefPubMedGoogle Scholar
  19. Kisseleva T, Bhattacharya S, Braunstein J, Schindler CW (2002) Signaling through the JAK/STAT pathway, recent advances and future challenges. Gene 285:1–24CrossRefPubMedGoogle Scholar
  20. Littell RC, Henry PR, Ammerman CB (1998) Statistical analysis of repeated measures data using SAS procedures. J Anim Sci 76:1216–1231CrossRefPubMedGoogle Scholar
  21. Liu X, Robinson GW, Gouilleux F, Groner B, Hennighausen L (1995) Cloning and expression of Stat5 and an additional homologue (Stat5b) involved in prolactin signal transduction in mouse mammary tissue. Proc Natl Acad Sci USA 92:8831–8835CrossRefPubMedPubMedCentralGoogle Scholar
  22. McCracken JY, Molenaar AJ, Snell RJ, Davey HW, Wilkins RJ (1997) A polymorphic TG repeat present within the bovine STAT5A gene. Anim Genet 28:453–464CrossRefGoogle Scholar
  23. Moleenar A, Wheeler TT, McCracken JY, Seyfert H (2000) The STAT3- encoding gene resides within the 40 kbp gap between the STAT5A- and STAT5B-encoding genes in cattle. Anim Genet 31:333–346CrossRefGoogle Scholar
  24. Peretti V, Ciotola F, Iannuzzi L (2013) Characterization, conservation and sustainability of endangered animal breeds in Campania (Southern Italy). Nat Sci 5:1–9Google Scholar
  25. Sadeghi M, Shahrbabak MM, Mianji GR, Javaremi AN (2009) Polymorphism at locus of STAT5A and its association with breeding values of milk production traits in Iranian Holstein bulls. Livest Sci 123:97–100CrossRefGoogle Scholar
  26. Sartore S, Barbieri V, Rasero R, Sacchi P, Di Stasio L, Sartore G (2005) Analysis of genetic variation in Agerolese cattle breed. Biochem Genet 43:485–490CrossRefPubMedGoogle Scholar
  27. Selvaggi M, Dario C, Normanno G, Celano GV, Dario M (2009) Genetic polymorphism of STAT5A protein: relationships with production traits and milk composition in Italian Brown cattle. J Dairy Res 76:441–445CrossRefPubMedGoogle Scholar
  28. Selvaggi M, Tufarelli V, Pinto F, Centoducati G, Dambrosio A, Santacroce MP, Dario C (2013) Bovine STAT5A gene polymorphism analysis and its association with milk composition traits in Jersey cows. Intl J Biosci Biochem Bioinform 4:341–344Google Scholar
  29. Selvaggi M, D’Alessandro AG, Dario C (2016) Bovine STAT5A gene polymorphism and its influence on growth traits in Podolica breed. Anim Prod Sci 56:1056–1060CrossRefGoogle Scholar
  30. Seyfert H, Pitra C, Meyer L, Brunner RM, Wheeler TT, Molenaar A, McCracken JY, Herrmann J, Thiesen H, Schwerin M (2000) Molecular characterization of STAT5A- and STAT5B-encoding genes reveals extended intragenic sequence homogeneity in cattle and mouse and different degrees of divergent evolution of various domains. J Mol Evol 50:550–561CrossRefPubMedGoogle Scholar
  31. Suguisawa L, Souza AA, Oliveira HN, Curi RA, Silveira AC (2006) Relationship between growth hormone and STAT5A genes polymorphisms and beef cattle growth, carcass and meat quality traits. In: 8th world congress on genetics applied to livestock production, August 13–18, 2006, Belo HorizonteGoogle Scholar
  32. Wakao H, Gouilleux F, Groner B (1994) Mammary gland factor (MGF) is a novel member of the cytokine regulated transcription factor gene family and confers the prolactin response. EMBO J 13:2182–2191PubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Maria Selvaggi
    • 1
    Email author
  • Sara Albarella
    • 2
  • Cataldo Dario
    • 1
  • Vincenzo Peretti
    • 2
  • Francesca Ciotola
    • 2
  1. 1.Section of Veterinary Science and Animal Production, Department of DETOUniversity of Bari ‘Aldo Moro’ValenzanoItaly
  2. 2.Department of Veterinary Medicine and Animal ProductionUniversity Federico IINaplesItaly

Personalised recommendations