Biochemical Genetics

, Volume 54, Issue 1, pp 95–106 | Cite as

Association of Genetic Polymorphism in the Interleukin-8 Gene with Risk of Oral Cancer and Its Correlation with Pain

  • Prithvi Kumar Singh
  • Girish Chandra
  • Jaishri Bogra
  • Rajni Gupta
  • Vijay Kumar
  • Syed Rizwan Hussain
  • Amita Jain
  • Abbas Ali Mahdi
  • Mohammad Kaleem AhmadEmail author
Original Article


Oral cancer is a multifactorial disease process and involves complex interactions between gene to gene and gene to environmental factors. Interleukin 8 (IL-8), a pro-inflammatory cytokine, having angiogenic activity with elevated expression in tumor cells, is reported to play an essential role in oral cancer development. This study was conducted with the aim to investigate the role of IL-8 (-A251T) gene polymorphism in susceptibility, progression, and self-reporting pain in oral cancer. The single nucleotide polymorphisms of the IL-8 (-A251T) gene were screened in 300 patients with oral cancer and 300 healthy controls, by polymerase chain reaction-restriction fragment length polymorphism. Genotype and allele frequencies were evaluated by chi-square test and odds ratio (OR) with 95% confidence intervals (CIs) were used to evaluate the strength of associations. The results of the study demonstrated that IL-8 (-A251T) gene polymorphism was significantly associated with susceptibility of oral cancer, whereas its correlation with clinico-pathological status or pain due to oral cancer could not be established. The AT heterozygous (OR 5.31; CI 3.38–8.34; p 0.0001) and AA homozygous (OR 2.89; CI 1.76–4.75; p 0.0001) had a greater risk for oral cancer compared to TT homozygous. Furthermore, significantly increased values of A allele frequencies compared to T allele were observed in all patients (OR 1.56; CI 1.24–1.96; p 0.0002). Tobacco chewing and smoking were also found to influence the development of oral cancer and increased the incidence of pain in oral cancer patients. The findings of this study suggest that the IL-8 (-A251T) gene polymorphism may be associated with increased risk of oral cancer.


Cytokines Interleukin-8 Angiogenesis Pain Genotype 



This work was financially supported by a grant from Indian Council of Medical Research (ICMR) (No: 3/2/2/131/2012/NCD-III).

Compliance with Ethical Standards

Conflict of interest

The authors have no conflict of interests in this article.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Prithvi Kumar Singh
    • 1
  • Girish Chandra
    • 1
  • Jaishri Bogra
    • 1
  • Rajni Gupta
    • 1
  • Vijay Kumar
    • 2
  • Syed Rizwan Hussain
    • 3
  • Amita Jain
    • 4
  • Abbas Ali Mahdi
    • 3
  • Mohammad Kaleem Ahmad
    • 3
    Email author
  1. 1.Department of AnaesthesiologyKing George’s Medical UniversityLucknowIndia
  2. 2.Department of Surgical OncologyKing George’s Medical UniversityLucknowIndia
  3. 3.Department of BiochemistryKing George’s Medical UniversityLucknowIndia
  4. 4.Department of MicrobiologyKing George’s Medical UniversityLucknowIndia

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