Biochemical Genetics

, Volume 52, Issue 9–10, pp 387–402 | Cite as

Association Between IL-10 Gene Promoter Polymorphism and Hepatitis B Viral Infection in an Egyptian Population

  • Roba M. Talaat
  • Mahmoud F. Dondeti
  • Soha Z. El-Shenawy
  • Omaima A. Khamiss
Article

Abstract

Cytokines play critical roles in the pathogenesis of hepatitis B virus infection (HBV). This work was designed to study the effect of IL-10 gene polymorphisms (−1082G/A and −819C/T) on susceptibility of Egyptians to HBV. Genotyping was performed using single-stranded polymorphism-polymerase chain reaction in 118 Egyptian hepatitis B patients and 119 healthy controls, and IL-10 serum levels were measured using ELISA. The frequency of IL-10 −1082G/G was significantly higher in HBV patients than in healthy controls, and G/A and A/A were not significantly different between groups. The distribution of IL-10 −819 genotypes was not significantly different between the HBV and healthy control groups. Although AT was significantly different between controls and patients, the distribution of the other haplotypes was not. IL-10 levels were significantly lower among hepatitis B patients. Our data stress the importance of IL-10 gene polymorphism in HBV infection. Depending on our preliminary work, IL-10 −1082G/G may act as a host genetic factor in the susceptibility to HBV infection in Egyptians.

Keywords

IL-10 Hepatitis B Egyptians 

Notes

Acknowledgments

This work is part of a grant from the Egyptian Academy of Scientific Research and Technology (ASRT), Cairo, Egypt. The sponsors did not participate in the study design, in the collection, analysis, and interpretation of data, in the manuscript drafting, and in the decision to submit the article for publication.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Roba M. Talaat
    • 1
  • Mahmoud F. Dondeti
    • 1
  • Soha Z. El-Shenawy
    • 2
  • Omaima A. Khamiss
    • 3
  1. 1.Molecular Biology Department, Genetic Engineering and Biotechnology Research InstituteUniversity of Sadat CitySadat CityEgypt
  2. 2.Biochemistry Department, National Liver InstituteMenofia UniversityMenufiaEgypt
  3. 3.Animal Biotechnology Department, Genetic Engineering and Biotechnology Research InstituteUniversity of Sadat CitySadat CityEgypt

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