Population Genetic Structure of the Invasive Red Swamp Crayfish in China Revealed by ITS1 Variation
The invasive red swamp crayfish (Procambarus clarkii) provides a valuable opportunity for studying the population genetics of invasive species that disperse rapidly. We analyzed the population genetic structure among 12 populations of the crayfish in China based on the internal transcribed spacer 1 (ITS1) region. The ITS1 of 815 bp aligned across 34 haplotypes; the average GC content was 53.9%. AMOVA showed that intrapopulation variation (95.26%) was much higher than interpopulation variation (4.74%). Genetic differentiation between the Taiwan and mainland populations (F st = 0.160) was moderate, but the Chinese population (Taiwan and the mainland combined) and an American population were highly differentiated (0.682 and 0.977, respectively). Gene flow between the Chinese and American populations (N m = 0.006 and 0.117, respectively) was lower than that between Taiwan and the mainland (1.536). Phylogenetic trees showed that three major genealogical clusters matched the sample locations well, suggesting that genetic differentiation is created largely by geographic isolation.
KeywordsRed swamp crayfish Procambarus clarkii Invasive alien species Population genetic structure Internal transcribed spacer 1 (ITS1) region
We thank Xiang Huang and Lili Zhang for their assistance with the laboratory work. We also thank Jiahao Zhang for supplying his samples, Qianqian Sun (College of Life Sciences, Anhui Normal University, Wuhu, China) for her help in sequence analysis, and Prof. Huabin Zhao (Wuhan University, Wuhan, China) for his helpful suggestions. This research was supported by the National Natural Science Foundation of China (Grant no. 31172117) and the Anhui Natural Science Foundation (Grant no. 11040606M76) and the Graduate Student Innovation Research Projects of Anhui University.
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