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Biochemical Genetics

, Volume 51, Issue 3–4, pp 175–188 | Cite as

MicroRNAs as Markers for Neurally Committed CD133+/CD34+ Stem Cells Derived from Human Umbilical Cord Blood

  • Maryam Hafizi
  • Amir Atashi
  • Behnaz Bakhshandeh
  • Mahboubeh Kabiri
  • Samad Nadri
  • Reza Haji Hosseini
  • Masoud Soleimani
Article

Abstract

Neural differentiation of the CD133+/CD34+ subpopulation of human umbilical cord blood stem cells was investigated, and neuro-miR (mir-9 and mir-124) expression was examined. An efficient induction protocol for neural differentiation of hematopoietic stem cells together with the exclusion of retinoic acid in this process was also studied. Transcription of some neural markers such as microtubule-associated protein-2, beta-tubulin III, and neuron-specific enolase was evaluated by real-time PCR, immunocytochemistry, and western blotting. Increased expression of neural indicators in the treated cells confirmed the appropriate neural differentiation, which supported the high efficiency of our defined neuronal induction protocol. Verified high expression of neuro-miRNAs along with neuronal specific proteins not only strengthens the regulatory role of miRNAs in determining stem cell fate but also introduces these miRNAs as novel indicators of neural differentiation. These data highlight the prominent therapeutic potential of hematopoietic stem cells for use in cell therapy of neurodegenerative diseases.

Keywords

Hematopoietic stem cells MicroRNAs Neural differentiation CD133+/CD34+ 

Notes

Acknowledgments

This article was financially supported by the Stem Cell Technology Research Center.

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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Maryam Hafizi
    • 1
    • 3
  • Amir Atashi
    • 2
  • Behnaz Bakhshandeh
    • 4
  • Mahboubeh Kabiri
    • 1
    • 4
  • Samad Nadri
    • 1
    • 5
  • Reza Haji Hosseini
    • 3
  • Masoud Soleimani
    • 2
  1. 1.Stem Cell Biology DepartmentStem Cell Technology Research CenterTehranIran
  2. 2.Hematology Department, Faculty of Medical SciencesTarbiat Modares UniversityTehranIran
  3. 3.Biology DepartmentPayame Noor UniversityTehranIran
  4. 4.Department of Biotechnology, University College of ScienceUniversity of TehranTehranIran
  5. 5.Faculty of MedicineShahid Beheshti University of Medical SciencesTehranIran

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