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Prevalence and Molecular Identification of the Mediterranean Variant Among G6PD-Deficient Sistani and Balouch Males in Southeastern Iran

Abstract

To determine the prevalence of G6PD deficiency and a Mediterranean mutation among males in southeastern Iran, we studied 1,097 Sistani and Balouch schoolboys. A questionnaire was used to collect demographic data and a history of malaria infection; blood samples were evaluated for G6PD deficiency and the G6PD Mediterranean mutation. Of the 1,097 boys screened, 175 were G6PD deficient (5.8 % of the Sistani boys and 19.3 % of the Balouch boys). The malaria survey indicated that among Balouch subjects, malaria infection was about 14 times that of Sistani subjects. Molecular characterization of G6PD-deficient samples revealed a general frequency of 85.1 % for the Mediterranean variant among all subjects (75 % among Sistani and 86.2 % among Balouch cases). The high prevalence of G6PD deficiency among Balouch populations confirms the hypothesis that the distribution of G6PD deficiency is concordant with the geographic distribution of malaria.

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References

  1. Al-Allawi N, Eissa AA, Jubrael JMS, Jamal SAR, Hamamy H (2010) Prevalence and molecular characterization of glucose-6-phosphate dehydrogenase deficient variants among the Kurdish population of Northern Iraq. BMC Blood Disord 10(6):1–8

  2. Baird J, Hoffman S (2004) Primaquine therapy for malaria. Clin Infect Dis 39:1336–1345

  3. Bayoumi RA, Nur-E-Kamal MS, Tadayyon M, Mohamed KK, Mahboob BH, Qureshi MM, Lakani MS, Awaad MO, Kaeda J, Vulliamy TJ, Luzzatto L (1996) Molecular characterization of erythrocyte glucose-6-phosphate dehydrogenase deficiency in Al-Ain district, United Arab Emirates. Hum Hered 46:136–141

  4. Beutler E (2008) Glucose-6-phosphate dehydrogenase deficiency: a historical perspective. Blood 111:16–24

  5. Beutler E, Mitchell M (1968) Special modifications of the fluorescent screening method for glucose-6-phosphate dehydrogenase deficiency. Blood 32:816–818

  6. Beutler E, Vulliamy TJ (2002) Hematologically important mutations: glucose-6-phosphate dehydrogenase. Blood Cells Mol Dis 28:93–103

  7. Beutler E, Blume KG, Kaplan JC, Löhr GW, Ramot B, Valenline WN (1979) International committee for standardization in hematology: recommended screening test for glucose-6- phosphate dehyrogenase (G6PD) deficiency. Br J Hematol 43(3):456–459

  8. Cappellini M, Fiorelli G (2008) Glucose-6-phosphate dehydrogenase deficiency. Lancet 371(9606):64–74

  9. Daar S, Vulliamy T (1996) Molecular characterization of G6PD in Oman. Human Hered 49:172–174

  10. Edrissian GH (2006) Malaria in Iran: past and present situation. Iranian J Parasitol 1(1):1–14

  11. El-Hazmi MAF, Warsy AS (1990) Frequency of G6PD variants and deficiency in Arabia. Gene Georg 4:15–20

  12. Fanello CI, Karema C, Avellino P, Bancone G, Uwimana A, Lee SJ, d’Alessandro U, Modiano D (2008) High risk of severe anaemia after chlorproguanil–dapsone + artesunate antimalarial treatment in patients with G6PD (A−) deficiency. Plos One 3(12):e4031

  13. Gelpi AP (1965) G6PD deficiency in Saudi Arabia: a survey. Blood 25:486–493

  14. Gelpi AP, King MC (1977) New data on glucose-6-phosphate dehydrogenase deficiency in Saudi Arabia: G6PD variants and the association between enzyme deficiency and hemoglobins. Hum Hered 27(4):285–291

  15. Karimi M, Martinez di Montemuros F, Danielli MG, Farjadian S, Afrasiabi A, Fiorelli G, Cappellini MD (2003) Molecular characterization of glucose-6-phosphate dehydrogenase deficiency in the Fars province of Iran. Haematologica 88:346–347

  16. Luzzatto L (1979) Genetics of red cells and susceptibility to malaria. Blood 54:961–976

  17. Luzzatto L, Bienzle U (1979) The malaria/G6PD hypothesis. Lancet 1:1183–1184

  18. Martinez DI, Montemuros F, Dotti C, Tavazzi D, Fiorelli G, Cappellini M (1997) Molecular heterogeneity of glucose-6-phosphate dehydrogenase (G6PD) variants in Italy. Haematologica 82:440–445

  19. Mesbah-Namin SA, Sanati MH, Mowjoodi A, Mason PJ, Vulliamy TJ, Noori-Daloii M (2002) Three major glucose-6-phosphate dehydrogenase-deficient polymorphic variants identified in Mazandaran state of Iran. Br J Hematol 117:763–764

  20. Miri-Moghaddam E, Zadeh-Vakili A, Rouhani Z, Naderi M, Eshghi P, Khazaei Feizabad A (2011) Molecular basis and prenatal diagnosis of beta-thalassemia among Balouch population in Iran. Prenat Diagn 31(8):788–791

  21. Missiou T, Sagaraki S (1991) Screening for glucose 6-phosphate dehydrogenase deficiency as a preventive measure: prevalence among 1,286,000 Greek newborn infants. J Pediatr 119:293–299

  22. Mohammed AM, Al-Hilli F, Nadkarni KV, Bhagwa GP, Bapat JP (1992) Haemoglobinopathies and G6PD deficiency in hospital birth in Bahrain. Ann Saudi Med 12:536–539

  23. Moiz B, Nasir A, Moatter T, Ali Naqvi Z, Khurshid M (2009) Population study of 1311 C/T polymorphism of glucose-6 phosphate dehydrogenase gene in Pakistan: an analysis of 715 X-chromosomes. BMC Genet 10(41):1–7

  24. Mortazavi Y, Mirzamohammadi F, Teremahi Ardestani M, Miri-Moghaddam E, Vulliamy TJ (2010) Glucose 6-phosphate dehydrogenase deficiency in Tehran, Zanjan and Sistan-Balouchestan provinces: prevalence and frequency of Mediterranean variant of G6PD. Iran J Biotech 8(4):229–233

  25. Nkhoma E, Poole C, Vannappagari V, Hall S, Beutler E (2009) The global prevalence of glucose-6-phosphate dehydrogenase deficiency: a systematic review and meta-analysis. Blood Cells Mol Dis 42:267–278

  26. Noori-Daloii MR, Yousefi A, Mohammad Ganji S, Hejazi SH, Soltani S, Sanei Moghadam E et al (2005) Molecular identification of the most prevalent mutation of glucose-6-phosphate dehydrogenase gene in deficient patients in Sistani and Balochestan province of Iran. J Sci Islam Repub Iran 16(4):321–325

  27. Noori-Daloii MR, Hejazi SH, Yousefi A, Mohammad Ganji S, Soltani S, Javadi KR et al (2006a) Identification of mutations in G6PD gene in patients in Hormozgan Province of Iran. J Sci Islam Repub Iran 17(4):313–316

  28. Noori-Daloii MR, Soltanian S, Mohammad Gangi SH, Yousefi A, Hejazi S, Bani-hashem A et al (2006b) Molecular identification of the most prevalent mutations of glucose-6-posphate dehydrogenase (G6PD) gene in deficient patients in Khorasan province of Iran. J Sci Islam Repub Iran 17(2):103–106

  29. Oner R, Gümrük F, Acar C, Oner C, Gürgey A, Altay C (2000) Molecular characterization of glucose-6-phosphate dehydrogenase deficiency in Turkey. Haematologica 85:320–321

  30. Oppenheim A, Jury CL, Rund D, Vulliamy TJ, Luzzatto L (1993) G6PD Mediterranean accounts for the high prevalence of G6PD deficiency in Kurdish Jews. Hum Genet 91:293–294

  31. Rahimi Z, Vaisi-Raygani A, Nagel RL, Muniz A (2006) Molecular characterization of glucose-6-phosphate dehydrogenase deficiency in the Kurdish population of western Iran. Blood Cells Mol Dis 37:31–37

  32. Ruwende C, Hill A (1998) Glucose-6-phosphate dehydrogenase deficiency and malaria. J Mol Med 76:581–588

  33. Segeja MD, Mmbando BP, Kamugisha ML, Akida JA, Savaeli ZX, Minja DT, Msangeni HA, Lemnge MM (2008) Prevalence of glucose-6-phosphate dehydrogenase deficiency and haemoglobin S in high and moderate malaria transmission areas of Muheza, north-eastern Tanzania. Tanzan J Health Res 10(1):9–13

  34. Shaker Y, Onsi A, Aziz R (1966) The frequency of G6PD deficiency in the newborns and adults in Kuwait. Am J Hum Genet 18:609–613

  35. Theerathananon W, Francois JJ, Zongram O, Pumpaibool T, Hounnaklang N, Seugorn A, Siriporn N, Harnyuttanakorn P, Kanchanakhan N (2010) Prevalence of G6PD deficiency in malaria endemic area: case study in Bongti sub-district, Saiyok district, Kanchanaburi province, Thailand. J Health Res 24(1):55–62

  36. Vulliamy TJ, D’Urso M, Battistuzzi G, Estrada M, Foulkes NS, Martini G, Calabro V, Poggi V, Giordano R, Town M (1988) Diverse point mutations in the human glucose-6-phosphate dehydrogenase gene cause enzyme deficiency and mild or severe hemolytic anemia. Proc Natl Acad Sci USA 85(14):5171–5175

  37. White JM, Christi BS, Nam D, Daar S, Higgs DR (1993) Frequency and clinical significance of erythrocyte genetic abnormalities in Omanis. J Med Genet 30:396–400

  38. WHO Working Group (1989) Glucose-6-phosphate dehydrogenase deficiency. Bull World Health Organ 67:601–611

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Acknowledgments

The authors are grateful to the Deputy of Research in Zahedan University of Medical Sciences for financial support for this study and education offices in the regions where the study was carried out. We also thank all student subjects who kindly participated in this study.

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Correspondence to Ebrahim Miri-Moghaddam.

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Miri-Moghaddam, E., Mortazavi, Y., Nakhaee, A. et al. Prevalence and Molecular Identification of the Mediterranean Variant Among G6PD-Deficient Sistani and Balouch Males in Southeastern Iran. Biochem Genet 51, 131–138 (2013). https://doi.org/10.1007/s10528-012-9548-7

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Keywords

  • G6PD deficiency
  • Mediterranean mutation
  • Prevalence
  • Malaria