A Simple and Affordable Method of DNA Extraction from Fish Scales for Polymerase Chain Reaction
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Most fish studies today are performed at the molecular level, which needs reliable, quick, and cost-effective DNA extraction protocols (Liu et al. 2009; Ming et al. 2010; Shen et al. 2010). Generally, the most widely used materials for DNA extraction in fish are fins or red blood cell samples (Yang et al. 2001; Liu et al. 2009; Zhang et al. 2009; Li et al. 2009). As fins are used by fish in locomotion, their removal would adversely impact survival of sampled fish. Similarly, collection of samples of enough red blood for DNA extraction from small fish would require them to be cultured further, and this would also be difficult for the researcher. Thus, simpler methods of sample collection are needed for DNA extraction.
Since collection of fish scale samples is easy and noninvasive (Kumar et al. 2007), there is a need for a simple, rapid, and affordable method of DNA extraction from them. Based on a literature search, only five papers have described methods of DNA isolation...
KeywordsCatalytic Triad Calcium Salt Microsatellite Primer Fish Scale Megalobrama Amblycephala
This study was supported by grants from the Modern Agroindustry Technology Research System, “Staple Freshwater Fishery Industry Technology System” (No. CARS-46-05), Fundamental Research Funds for the Central Universities (No. 2011PY023 and 2011PY043), and Guangdong Haid Group Co., Ltd. Special thanks are given to Prof. Peter Edwards from the Asian Institute Technology, Thailand, for editing the manuscript.
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