Biochemical Genetics

, Volume 50, Issue 7–8, pp 549–559 | Cite as

An Interleukin-10 Gene Promoter Polymorphism (−592A/C) Associated with Type 2 Diabetes: A North Indian Study

  • Madhukar Saxena
  • C. G. Agrawal
  • Hemant Kumar Bid
  • Monisha BanerjeeEmail author


In this first report on the association of an IL-10 promoter polymorphism with type 2 diabetes mellitus in a North Indian population, the −592A/C SNP (rs1800872) was genotyped by PCR–RFLP and the IL-10 level measured using ELISA. Although no significant difference was observed in the genotypic frequencies (P = 0.657), diabetes patients carried a significantly higher number of A alleles at the −592 position, 25.6% (P < 0.001, odds ratio 0.887, 95% CI 0.670–1.184). Significant correlations were detected in postprandial glucose levels of CC-genotype patients and controls (P = 0.025), age and waist–hip ratio of CA patients and controls (P ≤ 0.001), and fasting glucose (P = 0.045) and low-density lipoprotein (P = 0.049) in all patients and controls. The serum IL-10 level was significantly higher in patients than in controls (P = 0.033). The polymorphism was significantly associated with disease incidence and its biochemical manifestations.


SNP Type 2 diabetes mellitus Interleukin-10 North Indian population ELISA PCR–RFLP 



The study was supported by a research grant from the Department of Biotechnology, New Delhi, India. Madhukar Saxena is thankful to the Indian Council of Medical Research for his Senior Research fellowship to carry out the study.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Madhukar Saxena
    • 1
  • C. G. Agrawal
    • 2
  • Hemant Kumar Bid
    • 3
  • Monisha Banerjee
    • 1
    Email author
  1. 1.Molecular and Human Genetics Laboratory, Department of ZoologyUniversity of LucknowLucknowIndia
  2. 2.Department of MedicineChhatrapati Shahuji Maharaj Medical UniversityLucknowIndia
  3. 3.Peter Houghton Lab, Center for Childhood CancerResearch Institute at Nationwide Children’s HospitalColumbusUSA

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