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Biochemical Genetics

, Volume 49, Issue 3–4, pp 251–257 | Cite as

Expression of Biologically Active Human Interferon Gamma in the Milk of Transgenic Mice Under the Control of the Murine Whey Acidic Protein Gene Promoter

  • Haydar Bagis
  • Digdem Aktoprakligil
  • Cagatay Gunes
  • Sezen Arat
  • Tolga Akkoc
  • Gaye Cetinkaya
  • Orhan Kankavi
  • Ali Cihan Taskin
  • Korhan Arslan
  • Munis Dundar
  • Vania L. Tsoncheva
  • Ivan G. Ivanov
Note

Introduction

Transgenic animals are used for production of recombinant proteins for scientific, pharmaceutical, and agricultural purposes. The ability of transgenic animals to produce biologically active recombinant proteins in an efficient and economic manner was demonstrated a long time ago and has attracted substantial attention and investments. Human interferon-γ (hIFN-γ) is a key cytokine endowed with multiple biological activities such as antiviral, antibacterial, antiparasitic, antiproliferative, and immunomodulatory activity (Smith et al. 1990; Tsanev and Ivanov 2001). Human IFN-γ is a glycoprotein naturally secreted by activated T lymphocytes and monocytes (Rinderknecht et al. 1984). It is a product of a single gene encoding a protein of 143 amino acids with a molecular mass of 20–25 kDa. The recombinant hIFN-γ expressed in Escherichia coli is nonglycosylated and, therefore, less stable in solution than the natural analog. To date the hIFN-γ gene has been successfully...

Keywords

Transgenic Mouse Mammary Gland Milk Sample Founder Mouse Slot Blot Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This study was conducted in the Tubitak, MRC, GEBI. The authors are grateful to Dr. Batu Erman for providing pTZ57R vector containing hIFN-γ. We thank Sakir Sekmen, Gazi Turgut, Arzu Tas, and Erman Ates for excellent technical assistance. This study was funded by Tubitak (SBAG-BULGAR-1-105S001) and Grant No. IDEI-02/30/17.12.09 from Bulgarian National Research for I. Ivanov.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Haydar Bagis
    • 1
    • 2
  • Digdem Aktoprakligil
    • 2
  • Cagatay Gunes
    • 3
  • Sezen Arat
    • 2
  • Tolga Akkoc
    • 2
  • Gaye Cetinkaya
    • 2
  • Orhan Kankavi
    • 4
  • Ali Cihan Taskin
    • 2
  • Korhan Arslan
    • 5
  • Munis Dundar
    • 5
  • Vania L. Tsoncheva
    • 6
  • Ivan G. Ivanov
    • 6
  1. 1.Medical Faculty, Medical Genetic DepartmentUniversity of AdiyamanAdiyamanTurkey
  2. 2.Tubitak MRC Genetic Engineering and Biotechnology Institute (GEBI)GebzeTurkey
  3. 3.Institute for Molecular MedicineUniversity of UlmUlmGermany
  4. 4.Veterinary Faculty, Biochemistry DepartmentMehmet Akif Ersoy UniversityBurdurTurkey
  5. 5.Medical Faculty, Medical Genetic DepartmentErciyes UniversityKayseriTurkey
  6. 6.Institute of Molecular BiologyBulgarian Academy of SciencesSofiaBulgaria

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