Expression of Biologically Active Human Interferon Gamma in the Milk of Transgenic Mice Under the Control of the Murine Whey Acidic Protein Gene Promoter
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Transgenic animals are used for production of recombinant proteins for scientific, pharmaceutical, and agricultural purposes. The ability of transgenic animals to produce biologically active recombinant proteins in an efficient and economic manner was demonstrated a long time ago and has attracted substantial attention and investments. Human interferon-γ (hIFN-γ) is a key cytokine endowed with multiple biological activities such as antiviral, antibacterial, antiparasitic, antiproliferative, and immunomodulatory activity (Smith et al. 1990; Tsanev and Ivanov 2001). Human IFN-γ is a glycoprotein naturally secreted by activated T lymphocytes and monocytes (Rinderknecht et al. 1984). It is a product of a single gene encoding a protein of 143 amino acids with a molecular mass of 20–25 kDa. The recombinant hIFN-γ expressed in Escherichia coli is nonglycosylated and, therefore, less stable in solution than the natural analog. To date the hIFN-γ gene has been successfully...
KeywordsTransgenic Mouse Mammary Gland Milk Sample Founder Mouse Slot Blot Analysis
This study was conducted in the Tubitak, MRC, GEBI. The authors are grateful to Dr. Batu Erman for providing pTZ57R vector containing hIFN-γ. We thank Sakir Sekmen, Gazi Turgut, Arzu Tas, and Erman Ates for excellent technical assistance. This study was funded by Tubitak (SBAG-BULGAR-1-105S001) and Grant No. IDEI-02/30/17.12.09 from Bulgarian National Research for I. Ivanov.
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