Identification of a QTL for Adipocyte Volume and of Shared Genetic Effects with Aspartate Aminotransferase
- 68 Downloads
Plasma levels of aspartate aminotransferase (AST), a liver enzyme, are elevated in patients with visceral obesity. This study examined whether adipocyte volume is under the influence of genetic factors and evaluated its genetic correlations with AST. Fasting plasma levels of 344 pedigreed baboons from the Southwest National Primate Research Center in San Antonio, TX, USA, were assayed for AST. Adipocyte volume was measured using biopsies of omental adipose tissue. Adipocyte volume, body weight, and plasma AST were heritable. Genetic correlations between the measured adiposity-related phenotypes and AST were significant. A quantitative trait locus (LOD score 3.2) for adipocyte volume was identified on the baboon homolog of human chromosome 6 near marker D6S1028. These results suggest that omental adipocyte volume is under genetic regulation and that shared genetic factors influence adiposity-associated traits and AST.
KeywordsNonalcoholic fatty liver disease Obesity Adipocyte size Genome scan QTL Aspartate aminotransferase
This investigation was conducted in part in facilities constructed with support from the Research Facilities Improvement Program under grants C06 RR014578, C06 RR013556, C06 RR015456, and C06 RR017515 and with support from NIH grants PO1 HL028972, P51 RR013986, and R01 MH59490, as well as research support from the Kronkosky Charitable Foundation.
- Bose T, Lopez-Alvarenga JC, Dick E, Tejero ME, Freeland-Graves JH, Cole SA, Comuzzie AG (2006) Presence of non-alcoholic fatty liver in baboons. Am J Primatol Suppl 68(1):41Google Scholar
- Cancello R, Tordjman J, Poitou C, Guilhem G, Bouillot JL, Hugol D, Coussieu C, Basdevant A, Bar Hen A, Bedossa P, Guerre-Millo M, Clément K (2006) Increased infiltration of macrophages in omental adipose tissue is associated with marked hepatic lesions in morbid human obesity. Diabetes 55:1554–1561CrossRefPubMedGoogle Scholar
- Eguchi Y, Eguchi T, Mizuta T, Ide Y, Yasutake T, Iwakiri R, Hisatomi A, Ozaki I, Yamamoto K, Kitajima Y, Kawaguchi Y, Kuroki S, Ono N (2006) Visceral fat accumulation and insulin resistance are important factors in nonalcoholic fatty liver disease. J Gastroenterol 41:462–469CrossRefPubMedGoogle Scholar
- Maeda K, Cao H, Kono K, Gorgun CZ, Furuhashi M, Uysal KT, Cao Q, Atsumi G, Malone H, Krishnan B, Minokoshi Y, Kahn BB, Parker RA, Hotamisligil GS (2005) Adipocyte/macrophage fatty acid binding proteins control integrated metabolic responses in obesity and diabetes. Cell Metab 1:107–119CrossRefPubMedGoogle Scholar
- Marchesini G, Avagnina S, Barantani EG, Ciccarone AM, Corica F, Dall’Aglio E, Dalle Grave R, Morpurgo PS, Tomasi F, Vitacolonna E (2005) Aminotransferase and gamma-glutamyltranspeptidase levels in obesity are associated with insulin resistance and the metabolic syndrome. J Endocrinol Investig 28:333–339Google Scholar
- Mendez-Sanchez N, Chavez-Tapia NC, Medina-Santillan R, Villa AR, Sanchez-Lara K, Ponciano-Rodriguez G, Ramos MH, Uribe M (2006) The efficacy of adipokines and indices of metabolic syndrome as predictors of severe obesity-related hepatic steatosis. Dig Dis Sci 51:1716–1722CrossRefPubMedGoogle Scholar
- Relling DP, Esberg LB, Fang CX, Johnson WT, Murphy EJ, Carlson EC, Saari JT, Ren J (2006) High-fat diet-induced juvenile obesity leads to cardiomyocyte dysfunction and upregulation of Foxo3a transcription factor independent of lipotoxicity and apoptosis. J Hypertens 24:549–561CrossRefPubMedGoogle Scholar