Identification of NF-κB1 and NF-κBIΑ Polymorphisms Using PCR–RFLP Assay in a Turkish Population
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A polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) assay was used in a Turkish population to determine the frequency of polymorphisms of the nuclear factor-kappa (NF-κB1) and NF-κBIA genes, which have been shown to be related to several inflammatory diseases and cancer pathogenesis. Total genomic DNA was isolated from peripheral blood samples taken from 565 healthy volunteers living in Aydın Province. The genomic regions in question were amplified by PCR, and the polymorphisms in these regions were detected by a PCR–RFLP assay. The frequencies were 10.3% for the NF-κB1 −94ins/delATTG del/del genotype, 49.1% for del/ins, and 40.6% for ins/ins. The genotype frequencies of the NF-κBIA 3′UTR A → G genotypes were A/A 19.2%, A/G 42.3%, and G/G 38.5%. Distribution of genotype frequencies was tested by Hardy–Weinberg; the NF-κB1 gene was in Hardy–Weinberg equilibrium (χ2 = 3.402, P > 0.05), the NF-κBIA gene was not (χ2 = 8.293, P < 0.05).
KeywordsNF-κB1 NF-κBIA PCR–RFLP Genetic polymorphism Turkish
We thank the Turkish Red Crescent for permission to collect blood samples from volunteer blood donors. This work was financially supported by the Scientific Research Projects Unit of Adnan Menderes University, Turkey.
- Glas J, Török HP, Tonenchi L, Müller-Myhsok B, Mussack T, Wetzke M, Klein W, Epplen JT, Griga T, Schiemann U, Lohse P, Seiderer J, Schnitzler F, Brand S, Ochsenkühn T, Folwaczny M, Folwaczny C (2006) Role of the NFKB1–94ins/delATTG promoter polymorphism in IBD and potential interactions with polymorphisms in the CARD15/NOD2, IKBL, and IL-1RN genes. Inflamm Bowel Dis 12(7):606–611CrossRefPubMedGoogle Scholar
- Karban AS, Okazaki T, Panhuysen CIM, Gallegos T, Potter JJ, Bailey-Wilson JE, Silverberg MS, Duerr RH, Cho JH, Gregersen PK, Wu Y, Achkar JP, Dassopoulos T, Mezey E, Bayless TM, Nouvet FJ, Brant SR (2004) Functional annotation of a novel NFKB1 promoter polymorphism that increases risk for ulcerative colitis. Hum Mol Genet 13(1):35–45CrossRefPubMedGoogle Scholar
- Kurylowicz A, Hiromatsu Y, Jurecka-Lubieniecka B, Kula D, Kowalska M, Ichimura M, Koga H, Kaku H, Bar-Andziak E, Nauman J, Jarzab B, Plosk R, Bednarczuk T (2007) Association of NFKB1–94ins/del ATTG promoter polymorphism with susceptibility to and phenotype of Graves’ disease. Genes Immun 8:532–538CrossRefPubMedGoogle Scholar
- Mirza MM, Fisher SA, Onnie C, Lewis CM, Mathew CG, Sanderson J, Forbes A (2005) No association of the NFKB1 promoter polymorphism with ulcerative colitis in a British case control cohort. Br Med J 54:1205–1206Google Scholar
- Ulusoy M, Tunçbilek E (1987) Türkiye’de akraba evlilikleri ve çocuk ölümlerine etkisi. Nüfusbilim Derg 9:7–26Google Scholar