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Biochemical Genetics

, Volume 47, Issue 5–6, pp 397–411 | Cite as

Novel Mutations of the FASN Gene and Their Effect on Fatty Acid Composition in Japanese Black Beef

  • Tsuyoshi Abe
  • Junich Saburi
  • Hiroyuki Hasebe
  • Tetsuo Nakagawa
  • Satsuki Misumi
  • Toshihiro Nade
  • Hiroaki Nakajima
  • Noriaki Shoji
  • Masato Kobayashi
  • Eiji Kobayashi
Article

Abstract

Eight novel and four known mutations were detected in the coding sequence of the bovine fatty acid synthase (FASN) gene of an F2 population from Japanese Black and Limousin cattle. Two mutations, g.16024A>G and g.16039T>C, detected in exon 34, which determine amino acid substitutions of threonine (T) to alanine (A) and tryptophan (W) to arginine (R), were clearly separated in the parental breeds. The haplotypes (TW and AR) segregated in F2 individuals and had a significant effect on the fatty acid composition of backfat, intermuscular fat, and intramuscular fat. The TW haplotype was associated with increasing C18:0 and C18:1 content and the ratio of monounsaturated to saturated fatty acids, and decreasing C14:0, C14:1, C16:0, and C16:1 content. The two mutations were screened in two commercial Japanese Black half-sibling populations and similarly determined the contribution to the fatty acid composition of intramuscular fat. The frequency of the TW haplotype was markedly higher in Japanese Black (0.67) than in Holstein (0.17), Angus (0.02), and Hereford (0.07). We conclude that these mutations may contribute to the characteristic fatty acid composition of Japanese Black beef.

Keywords

Bovine Fatty acid composition Fatty acid synthase Japanese Black beef 

Notes

Acknowledgments

The authors thank the Shirakawa Institute of Animal Genetics for utilization of their automatic DNA extraction equipment, and the staff of the Tokachi and Ohu branches of the NLBC for meat sample collection from F2 animals.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Tsuyoshi Abe
    • 1
  • Junich Saburi
    • 1
  • Hiroyuki Hasebe
    • 1
  • Tetsuo Nakagawa
    • 1
  • Satsuki Misumi
    • 1
  • Toshihiro Nade
    • 2
  • Hiroaki Nakajima
    • 3
  • Noriaki Shoji
    • 3
  • Masato Kobayashi
    • 3
  • Eiji Kobayashi
    • 1
  1. 1.National Livestock Breeding CenterFukushimaJapan
  2. 2.Nippon Veterinary and Life Science UniversityTokyoJapan
  3. 3.Yamagata Prefectural Animal Industrial InstituteAgricultural Research CenterYamagataJapan

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