Biochemical Genetics

, Volume 47, Issue 1–2, pp 66–79 | Cite as

Isolation and Characterization of Testis-Specific DMRT1 in the Tropical Abalone (Haliotis asinina)

  • Sirawut Klinbunga
  • Piti Amparyup
  • Bavornlak Khamnamtong
  • Ikuo Hirono
  • Takashi Aoki
  • Padermsak Jarayabhand
Article

Abstract

The Doublesex Male abnormal-3 Related Transcription factor-1 (DMRT1) gene encodes a protein containing the DNA-binding motif called the DM domain, involved in the sexual development of various species. To gain insight into its implications for gonadal differentiation in the tropical abalone (Haliotis asinina), a DMRT1 homolog was identified and characterized. The full length cDNA of HADMRT1 (1,740 bp with an ORF of 732 bp corresponding to a putative polypeptide of 243 amino acids) and its DM domain-less variant (HADMRT1-like, 1,430 bp with an ORF of 312 bp, 103 amino acids) were successfully isolated and reported for the first time in molluscs. HADMRT1 was specifically expressed in the testes of adult H. asinina (N = 16) but not in whole juveniles (2, 3, 5 months old, N = 6 for each group) and ovaries (N = 16), and pooled hemocytes (from 50 individuals) of adults. Tissue distribution analysis further revealed testis-specific expression of HADMRT1. Semiquantitative RT-PCR illustrated that the relative expression level of HADMRT1 in developed testes (stages II, III, and IV) was significantly greater than that in undeveloped testes (stage I) of abalone broodstock (P < 0.05).

Keywords

RACE-PCR Testis-specific expression DMRT1 DM domain-less Abalone 

Notes

Acknowledgments

This work was supported by a Basic Research Grant for the Royal Golden Jubilee PhD Program, the Thailand Research Funds (TRF), awarded to PA.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Sirawut Klinbunga
    • 1
    • 2
  • Piti Amparyup
    • 3
  • Bavornlak Khamnamtong
    • 1
    • 2
  • Ikuo Hirono
    • 4
  • Takashi Aoki
    • 4
  • Padermsak Jarayabhand
    • 5
    • 6
  1. 1.Aquatic Molecular Genetics and Biotechnology Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC)National Science and Technology Development Agency (NSTDA)Klong LuangThailand
  2. 2.Center of Excellence for Marine Biotechnology, Faculty of ScienceChulalongkorn UniversityBangkokThailand
  3. 3.Program in Biotechnology, Faculty of ScienceChulalongkorn UniversityBangkokThailand
  4. 4.Laboratory of Genome Science, Graduate School of Marine Science and TechnologyTokyo University of Marine Science and TechnologyTokyoJapan
  5. 5.Department of Marine Science, Faculty of ScienceChulalongkorn UniversityBangkokThailand
  6. 6.Aquatic Resources Research InstituteChulalongkorn UniversityBangkokThailand

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