Biochemical Genetics

, Volume 46, Issue 5–6, pp 362–368 | Cite as

Novel SNPs of the mtDNA ND5 Gene and Their Associations with Several Growth Traits in the Nanyang Cattle Breed

  • Bao Zhang
  • Hong Chen
  • Liushuai Hua
  • Chunlei Zhang
  • Xiangtao Kang
  • Xinzhuang Wang
  • Chuanying Pan
  • Xianyong Lan
  • Chuzhao Lei
Article

Abstract

Polymorphism of the mtDNA ND5 gene was detected by PCR-SSCP and DNA sequencing methods in 714 individuals from six Chinese cattle breeds. The results showed that at the P2 locus, two kinds of haplotypes, named A and B, with three SNPs (T12900C, A12923T, C12924T) were detected. The frequencies of haplotype A in six breeds were 0.041–0.167. Polymorphism of the ND5 gene was shown to be associated with growth in the Nanyang breed. Individuals with haplotype B had greater hucklebone width than those with haplotype A when 6 months old (P < 0.01), as well as better body height, body length, body weight, and average daily gain at 6 months (P < 0.05). Therefore, haplotype B of the ND5 gene was likely to have a positive effect on growth traits at 6 months in the Nanyang breed.

Keywords

Cattle mtDNA ND5 gene Polymorphism Single nucleotide polymorphism (SNP) Growth traits 

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Bao Zhang
    • 1
  • Hong Chen
    • 1
    • 2
  • Liushuai Hua
    • 1
  • Chunlei Zhang
    • 1
  • Xiangtao Kang
    • 1
  • Xinzhuang Wang
    • 3
  • Chuanying Pan
    • 1
  • Xianyong Lan
    • 1
  • Chuzhao Lei
    • 1
  1. 1.College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for AgricultureNorthwest A&F UniversityYanglingP.R. China
  2. 2.Institute of Cellular and Molecular BiologyXuzhou Normal UniversityXuzhouP.R. China
  3. 3.Research Center of Cattle Engineering Technology in HenanZhengzhouP.R. China

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