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Biochemical Genetics

, Volume 46, Issue 5–6, pp 312–322 | Cite as

Bioinformatics Analysis of Lactoferrin Gene for Several Species

  • Jing-Fen Kang
  • Xiang-Long LiEmail author
  • Rong-Yan Zhou
  • Lan-Hui Li
  • Fu-Jun Feng
  • Xiu-Li Guo
Article

Abstract

Much attention has been focused on the study of lactoferrin due to its function in antibacterial, antiviral, antifungal, anti-inflammatory, anti-oxidant, and immunomodulatory activities. A total of 60 lactoferrin (LF) gene sequences with the complete coding regions (CDS) and corresponding amino acids belonging to 11 species were analyzed, and the differentiation within and among the species was also studied. The results showed that most of the species have the stop codon TAA, with the variation of TGA for Mus musculus. The length of the LF gene with the complete CDS varies greatly, from 2,055 to 2,190 bp, due to deletion, insertion, and stop codon mutation resulting in elongation. Observed genetic diversity was higher among species than within species, and Sus scrofa had more polymorphisms than any other species. Novel amino acid variation sites were detected within several species (8 in Homo sapiens, 6 in Mus musculus, 6 in Capra hircus, 10 in Bos taurus, and 20 in Sus scrofa), which might be used to illustrate the functional variation. Differentiation of the LF gene was obvious among species, and the clustering result was consistent with the taxonomy in the National Center for Biotechnology Information.

Keywords

LF gene Variation Species 

Notes

Acknowledgments

We are grateful to the reviewer for valuable suggestions and comments on the manuscript. This work was funded by the subproject of Breeding System Establishment Production in Scale of Dairy Cow with High Performance: Breeding System Establishment of Dairy Goat (No. 2006BAD04A10-06).

References

  1. Arnason U, Gullberg A, Gretarsdottir S, Ursing B, Janke A (2000) The mitochondrial genome of the sperm whale and a new molecular reference for estimating eutherian divergence dates. Mol Evol 50(6):569–578Google Scholar
  2. Bellamy W, Takase M, Yamauchi K, Wakabayashi H, Kawase K, Tomita M (1992) Identification of the bactericidal domain of LF. Biochim Biophys Acta 1121:130–136PubMedGoogle Scholar
  3. Berlov MN, Korableva ES, Andreeva YV, Ovchinnikova TV, Kokryakov VN (2007) Lactoferrin from canine neutrophils: isolation and physicochemical and antimicrobial properties. Biochemistry (Moscow) 4:551–559Google Scholar
  4. Cai PL, Tong Y, Wang XQ, Zhang YZ (2005) Cloning and sequence analysis of human LF. J Sichuan Univ 42:1229–1232Google Scholar
  5. Cohen MS, Britigan BE, French M, Bean K (1987) Preliminary observations on LF secretion in human vaginal mucus: Variation during the menstrual cycle, evidence of hormonal regulation, and implications for infection with Neisseria gonorrhoeae. Am J Obstet Gynecol 157:1122–1125PubMedGoogle Scholar
  6. Delsuc F, Vizcaíno SF, Douzery EJ (2004) Influence of tertiary paleoenvironmental changes on the diversification of South American mammals: a relaxed molecular clock study within xenarthrans. BMC Evol Biol 4:11CrossRefPubMedGoogle Scholar
  7. Ghosh T (2000) Studies on codon usage in Entamoeba histolytica. Int J Parasitol 30:715–722CrossRefPubMedGoogle Scholar
  8. Kabilan V, Jeffrey B, Kaplan, David F (2003) One of two human LF variants exhibits increased antibacterial and transcriptional activation activities and is associated with localized juvenile periodontitis. Infect immun 11:6141–6147Google Scholar
  9. Kumar S, Hedges SB (1998) A molecular timescale for vertebrate evolution. Nature 392(6679):917–920CrossRefPubMedGoogle Scholar
  10. Li GH, Zhang Y, Li N (2001) Analysis on the partial sequence of bovine LF gene by PCR-SSCP. J Agric Biotechnol 9(2):139–141Google Scholar
  11. Li WH, Dan G (1991) Expand fundamentals of molecular evolution. Sinauer Associates, SunderlandGoogle Scholar
  12. Li XL, Zheng GR, Zhou RY (2007) Evolution and differentiation of MSHR gene in different species. J Hered 3:165–168CrossRefGoogle Scholar
  13. Liu T, Zhang YZ, Wu XF (2005) cDNA cloning of human LF and its expression in BmN Cells. Sci Seric 31(3):280–285Google Scholar
  14. Lynch M, Crease TJ (1990) The analysis of population survey data on DNA sequence variation. Mol Biol Evol 7:377–394PubMedGoogle Scholar
  15. Mohamed JA, DuPont HL, Jiang ZD (2007) A novel single-nucleotide polymorphism in the LF gene is associated with susceptibility to diarrhea in North American travelers to Mexico. Clin Infect Dis 44(7):945–952CrossRefPubMedGoogle Scholar
  16. Nikaido M, Kawai K, Cao Y, Harada M, Tomita S, Okada N, Hasegawa M (2001) Maximum likelihood analysis of the complete mitochondrial genomes of eutherians and a reevaluation of the phylogeny of bats and insectivores. Mol Evol 53(4–5):508–516CrossRefGoogle Scholar
  17. Robert FW (2001) Molecular biology. University of Kansas-Lawrence, Scientific PressGoogle Scholar
  18. Strom MB, Hang BE, Redal, Skar ML, Stensen W, Svendsen JS (2002) Important structural features of 15-residue lactoferricin derivatives and methods for improve of antimicrobial activity. Biochem Cell Biol 80(1):65–74CrossRefPubMedGoogle Scholar
  19. Tajima F (1983) Evolutionary relationship of DNA sequences in finite populations. Genetics 105:437–460PubMedGoogle Scholar
  20. Tang B, Cao GF, Yang YF (2006) Developments of RACE assay for amplification of full length sequence of camel β-defensin cDNA. Vet Sci China 36(2):151–156Google Scholar
  21. Tomita M, Bellamy W, Takase M, Yamauchi K, Wakabayashi H, Kawase K (1991) Potent antibacterial peptides generated by pepsin digestion of bovine LF. Dairy Sci 74:4137–4142CrossRefGoogle Scholar
  22. Vogel HJ, Schibli D, Jing W, Lohmeier-Vogel EM, Epand RF, Epand RM (2002) Towards a structure-function analysis of bovine lactoferricin and related tryptophan and arginine containing peptides. Biochem Cell Biol 80:49–63CrossRefPubMedGoogle Scholar
  23. Wang Y, Wu X, Liu G, Cao C, Huang H, Xu Z, Liu J (2005) Expression of porcine lactoferrin by using recombinant baculovirus in silkworm, Bombyx mori L and its purification and characterization. Appl Microbiol Biotechnol 69(4):385–389CrossRefPubMedGoogle Scholar
  24. Wildman DE, Uddin M, Liu G, Grossman LI, Goodman, M (2003) Implications of natural selection in shaping 99.4% nonsynonymous DNA identity between humans and chimpanzees: enlarging genus Homo. Proc Natl Acad Sci USA 100(12):7181–7188CrossRefPubMedGoogle Scholar
  25. Yang G, Pang QF, Wu CY (2005) Cloning of human LF gene and analysis of the polymorphism in its sequence. Acta Acad Med Xuzhou 25(5):390–392Google Scholar
  26. Yang YF, Tang B, Cao GF (2004) The cDNA cloning and sequencing of camel B-defensin caBD-1. Acta Vet Zootech Sin 35(4):357–361Google Scholar
  27. Yang Z, Yoder AD (2003) Comparison of likelihood and Bayesian methods for estimating divergence times using multiple gene Loci and calibration points, with application to a radiation of cute-looking mouse lemur species. Syst Biol 52(5):705–716CrossRefPubMedGoogle Scholar
  28. Zhang R, Diao QY (2007) Application of lactoferrin on calves and bioinformatics analysis. J Dairy Sci Technol 1:39–42Google Scholar
  29. Zhou L (2006) PCR-SSCP analysis on the partial sequence of Blf and the expression of its feasibility as molecular marker of mastitis resistance. Nanjing, Agricultural UniversityGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Jing-Fen Kang
    • 1
  • Xiang-Long Li
    • 1
    Email author
  • Rong-Yan Zhou
    • 1
  • Lan-Hui Li
    • 1
  • Fu-Jun Feng
    • 1
  • Xiu-Li Guo
    • 1
  1. 1.College of Animal Science and TechnologyAgricultural University of HebeiBaodingP.R. China

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