Biochemical Genetics

, Volume 45, Issue 1–2, pp 145–153 | Cite as

MspI Allelic Pattern of Bovine Growth Hormone Gene in Indian Zebu Cattle (Bos indicus) Breeds

  • M. Sodhi
  • M. Mukesh
  • B. Prakash
  • B. P. Mishra
  • R. C. Sobti
  • Karn P. Singh
  • Satbir Singh
  • S. P. S. Ahlawat
Article
First Online:

The MspI allelic variation in intron III of the bovine growth hormone (bGH) gene was explored using PCR-RFLP in 750 animals belonging to 17 well-recognized breeds of Indian zebu cattle (Bos indicus) reared in different geographic locations of the country. Restriction digestion analysis of a 329-bp PCR fragment of the bGH intron III region with MspI restriction enzyme revealed two alleles (MspI− and MspI+) and two genotypes (−/− and +/−) across the 17 cattle breeds studied. The allelic frequency varied from 0.67 to 0.94 for MspI (−) and from 0.06 to 0.33 for MspI (+) across the 17 breeds, with a combined average frequency of 0.87 and 0.13, respectively. No animal with +/+ genotype was detected across the samples analyzed. The chi-square test showed that the difference in MspI allelic frequency was not significant (p > 0.05), regardless of the geographic origin, coat color, or utility of the cattle breed. The high MspI (−) allele frequencies obtained for Indian zebu cattle in this study are in sharp contrast to those reported for taurine breeds from northern Europe, Mediterranean countries, and America. Findings of this study further substantiate the hypothesis that the MspI (−) allele has an Indian origin.

KEY WORDS

Indian zebu cattle bovine growth hormone gene genetic variation MspI allele 
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Notes

ACKNOWLEDGMENTS

The financial support received from the Indian Council of Agriculture Research, New Delhi, India, for this work is duly acknowledged. We thank the NBAGR Core Lab, Chennai, under the ICAR network project, for providing DNA samples of Amritmahal, Ongole, Kangyam, and Umblachery cattle. We also thank Mrs. Pravesh K. for rendering technical support during the course of this work.

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • M. Sodhi
    • 1
    • 2
  • M. Mukesh
    • 1
  • B. Prakash
    • 1
  • B. P. Mishra
    • 1
  • R. C. Sobti
    • 2
  • Karn P. Singh
    • 1
  • Satbir Singh
    • 1
  • S. P. S. Ahlawat
    • 1
  1. 1.Cattle Genomics LaboratoryNational Bureau of Animal Genetics ResourcesKarnalIndia
  2. 2.Department of BiotechnologyPanjab UniversityChandigarhIndia

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