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Biochemical Genetics

, Volume 44, Issue 9–10, pp 421–431 | Cite as

Genetic Variation of Chinese PRRSV Strains Based on ORF5 Sequence

  • Jian Chen
  • Tao Liu
  • Cheng-Gang Zhu
  • Yong-Feng Jin
  • Yao-Zhou Zhang
Original Paper

Thirteen isolates of porcine reproductive and respiratory syndrome virus (PRRSV) from different provinces of China were studied and compared with several PRRSV isolates from other countries. Phylogenetic analysis shows that all Chinese isolates of PRRSV in this study belong to the American genotype, except for one strain, B13, which clustered as a European genotype. Sequence analysis revealed that PRRSV Chinese isolates of the American genotype were highly similar in the ORF5 sequence and could be classified into two subclades. One contains PRRSV isolates that are more closely related to the American vaccine strain MLV Resp and its parent strain VR-2332, and the other contains ones only distantly related to them. Within the Chinese isolates slight genetic variation occurred, and some strains may originate directly from the vaccine virus.

KEY WORDS

PRRSV ORF5 RT-PCR phylogenetic analysis genetic variation 

Notes

ACKNOWLEDGMENTS

This work was supported by a grant from Zhejiang Natural Science Foundation of China (No. 398492).

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Jian Chen
    • 1
    • 2
  • Tao Liu
    • 1
    • 2
  • Cheng-Gang Zhu
    • 1
  • Yong-Feng Jin
    • 1
  • Yao-Zhou Zhang
    • 1
    • 2
  1. 1.College of Life SciencesZhejiang UniversityHangzhouP.R. China
  2. 2.Institute of BiochemistryZhejiang Sci-Tech UniversityHangzhouP.R. China

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