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Biochemical Genetics

, Volume 44, Issue 5–6, pp 270–285 | Cite as

Mitochondrial cytochrome b Sequence Variation and Phylogenetics of the Highly Specialized Schizothoracine Fishes (Teleostei: Cyprinidae) in the Qinghai-Tibet Plateau

  • Delin Qi
  • Taiping Li
  • Xinquan ZhaoEmail author
  • Songchang Guo
  • Junxiang Li
Article

Abstract

The complete 1140 bp mitochondrial cytochrome b sequences were obtained from 39 individuals representing five species of all four genera of highly specialized schizothoracine fishes distributed in the Qinghai-Tibet plateau. Sequence variation of the cytochrome b gene was surveyed among the 39 individuals as well as three primitive schizothoracines and one outgroup. Phylogenetic analysis suggested that the group assignment based on 1140 bp of the cytochrome b sequence is obviously different from previous assignments, and the highly specialized schizothoracine fishes (Schizopygopsis pylzovi, Gymnocypris przewalskii, G. eckloni, Chuanchia labiosa, and Platypharodon extremus) form a monophyletic group that is sister to the clade formed by the primitive schizothoracine fishes (Schizothorax prenanti, S. pseudaksaiensis, and S. argentatus). The haplotypes of Schizopygopsis pylzovi and G. przewalskii were paraphyletic based on cytochrome b data, which most likely reflected incomplete sorting of mitochondrial DNA lineages. The diploid chromosome numbers of Schizothoracinae were considered in phylogenetic analysis and provided a clear pattern of relationships. Molecular dating estimated for highly specialized schizothoracine fishes suggested that the highly specialized schizothoracine fishes diverged in the late Miocene Pliocene to Pleistocene (4.5 × 104–4.05 × 10years BP). The relationship between the cladogenesis of highly specialized schizothoracine fishes and geographical events of the Qinghai-Tibet plateau is discussed.

Keywords

Schizothoracinae mtDNA cytochrome b biogeography diploid chromosome numbers 

Notes

Acknowledgments

The authors thank Dr. B. W. Murray for valuable comments on the manuscript. This work was supported by the Ministry of Education Foundation for University Key Teachers and the National Natural Science Foundation of China (39860060). In order to extensively investigating the phylogenetic relationships of the subfamily Schizothoracinae, this work further supported by National Basic Research Project (No. 2005CB422005) in China.

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Delin Qi
    • 1
    • 2
  • Taiping Li
    • 3
  • Xinquan Zhao
    • 1
    Email author
  • Songchang Guo
    • 1
    • 2
  • Junxiang Li
    • 3
  1. 1.Northwest Plateau Institute of BiologyChinese Academy of ScienceXiningP.R. China
  2. 2.Graduate SchoolChinese Academy of ScienceBeijingP.R. China
  3. 3.College of Agriculture and Animal ScienceQinghai UniversityXiningP.R. China

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