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Biochemical Genetics

, Volume 44, Issue 3–4, pp 140–155 | Cite as

The Structure and Diversity of α1-Acid Glycoprotein/Orosomucoid Gene in Africans

  • Isao YuasaEmail author
  • Hiroaki Nakamura
  • Kazuo Umetsu
  • Yoshito Irizawa
  • Lotte Henke
  • Jürgen Henke
Original Article

Human orosomucoid (ORM), or α1-acid glycoprotein, is known to be controlled by duplicated and triplicated genes on chromosome 9, encoding ORM1 and ORM2 proteins. In this study, the structure and diversity of the ORM gene were investigated in 16 Sub-Saharan Africans, who originated from widely dispersed locations in Africa. The duplicated ORM1-ORM2 gene was observed in all 16 samples. ORM1*S1(2), characterized by an ORM2 gene-specific sequence in intron 5, was common in Africans. Three Africans showed the duplication of the ORM1 gene. The organization of the triplicated ORM1A-ORM1B-ORM2 gene was established in two Africans. The recombination breakpoints resulting in the ORM1 duplication lay within a small genomic interval around exon 1 of the ORM1B gene. The duplication of the ORM2 gene reported previously was not detected in this population sample. Several single-nucleotide polymorphisms were observed in the ORM2 gene. The rearrangement of the ORM gene is likely to occur often in Africans.

KEY WORDS:

α1-acid glycoprotein African gene rearrangement genomic structure orosomucoid 

Notes

ACKNOWLEDGMENTS

We would like to thank the late Prof. Satoshi Horai for providing some of the African DNA samples. This study was supported in part by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science.

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Isao Yuasa
    • 1
    • 4
    Email author
  • Hiroaki Nakamura
    • 2
  • Kazuo Umetsu
    • 3
  • Yoshito Irizawa
    • 1
  • Lotte Henke
    • 4
  • Jürgen Henke
    • 4
  1. 1.Division of Legal Medicine, Faculty of MedicineTottori UniversityYonagoJapan
  2. 2.Criminal Investigation LaboratoryShimane Prefectural Police HeadquartersMatsueJapan
  3. 3.Department of Experimental and Forensic Pathology, Faculty of MedicineYamagata UniversityYamagataJapan
  4. 4.Institut für BlutgruppenforschungCologneGermany

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