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Biological control of strawberry grey mold disease caused by Botrytis cinerea mediated by Colletotrichum acutatum extracts

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Abstract

Despite the negative impact on human, animal and environmental health, synthetic fungicides are the most common agrochemicals used to control Botrytis cinerea, the causal agent of grey mold disease. Strawberry plants are very susceptible to many pathogens, especially the necrotrophic fungus B. cinerea. In this work, we show that two fungal extracts obtained from a local isolate of Colletotrichum acutatum (M11) can protect strawberry plants against grey mold. Fungal culture filtrate (CF), and the axenic semi-purified culture filtrate (ACF) induce local and systemic acquired resistance against B. cinerea, and reduce fungal virulence. These results suggest that CF and ACF can be used as effective ingredients of bioproducts to control grey mold in strawberry crop. We also show that the elicitor peptide flg22 is effective to confer strawberry plants local and systemic protection against B. cinerea but only when applied 24 h prior to the infection.

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Abbreviations

FEs:

Fungal extracts

CF:

Culture filtrate

ACF:

Axenic culture filtrate

SAR:

Systemic acquired resistance

LAR:

Local acquired resistance

hpti:

Hours prior to infection

dpi:

Days post-infection

hpt:

Hours post-treatment

FW:

Fresh weight

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Acknowledgements

Authors are grateful to Strawberry Active Germplasm Bank (BGA) from Universidad Nacional de Tucuman (UNT), Cecilia Lemme, for providing strawberry plants, and Debes M for technical assistance. RHTG, VHC, FJRS, MP and MdPC are CONICET fellowships, and SMS and JCDR are members of CONICET.

Funding

This study was funded by grants of the Universidad Nacional de Tucumán (PIUNT 26/D642), Agencia Nacional de Promoción Científica y Tecnológica (PICT 2017-0653), and CONICET (PUE-2016-0104).

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Author notes

  1. R. H. Tomas-Grau and V. Hael-Conrad have equally contributed to the paper.

Authors and Affiliations

  1. Instituto Superior de Investigaciones Biológicas (INSIBIO, CONICET-UNT), Universidad Nacional de Tucumán, Chacabuco 461, T4000ILI, Tucumán, Argentina

    R. H. Tomas-Grau, V. Hael-Conrad, F. J. Requena-Serra, S. M. Perato, M. d. P. Caro & J. C. Díaz-Ricci

  2. INTA EEA Famaillá, Ruta Prov. 301 Km 32, Famaillá, 4132, Tucumán, Argentina

    V. Hael-Conrad, M. d. P. Caro & S. M. Salazar

Authors
  1. R. H. Tomas-Grau
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  2. V. Hael-Conrad
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  3. F. J. Requena-Serra
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  4. S. M. Perato
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  5. M. d. P. Caro
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  6. S. M. Salazar
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  7. J. C. Díaz-Ricci
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Corresponding author

Correspondence to J. C. Díaz-Ricci.

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Handling Editor: Jesus Mercado Blanco.

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Tomas-Grau, R.H., Hael-Conrad, V., Requena-Serra, F.J. et al. Biological control of strawberry grey mold disease caused by Botrytis cinerea mediated by Colletotrichum acutatum extracts. BioControl 65, 461–473 (2020). https://doi.org/10.1007/s10526-020-10003-4

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  • DOI: https://doi.org/10.1007/s10526-020-10003-4

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