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Comparative analysis of blastospore production and virulence of Beauveria bassiana and Cordyceps fumosorosea against soybean pests

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Abstract

Blastospores are yeast-like infective cells to arthropod pests and can be obtained shortly by liquid culture. However, fungal isolates exhibit striking variation in production, desiccation tolerance and virulence. In this study, we screened three isolates of Beauveria bassiana (Balsamo) Vuillemin and three of Cordyceps fumosorosea (Wize) (Hypocreales: Cordycipitaceae) for blastospore production and assessed desiccation tolerance by air drying. The virulence of these isolates were determined against Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) biotype B, Spodoptera frugiperda (J.E. Smith) and Chrysodeixis includens (Walker) (Lepidoptera: Noctuidae). The isolates ESALQ-1296 and ESALQ-3422 of C. fumosorosea and ESALQ-4958 of B. bassiana yielded blastospore concentrations above 1 × 109 ml−1, but only the two C. fumosorosea isolates retained viability rates above 75% after drying. Blastospores of both fungal species were more effective in killing the three insect species than aerial conidia. In summary, blastospores of C. fumosorosea ESALQ-1296 hold the best promise for broad-spectrum pest control in soybean.

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Acknowledgements

The first author thanks the São Paulo Research Foundation (FAPESP) [grant number 21387/2016–9] and the Coordination for the Improvement of Higher Educational Personnel (CAPES) for the scholarships. We also thank FAPESP [grant number 2018/02317-5] as part of the SPARCBIO (São Paulo Advanced Research Center for Biological Control) located at ESALQ/USP with support of FAPESP, Koppert and University of São Paulo (USP).

Author information

Bianca Corrêa and Italo Delalibera Júnior planned the experiments. Bianca Corrêa, Vanessa da Silveira Duarte and Daniela Milanez Silva performed the experiments. Gabriel Moura Mascarin analyzed the data. Bianca Corrêa, Gabriel Moura Mascarin and Italo Delalibera Júnior wrote the manuscript. All authors read and approved the final manuscript.

Correspondence to Gabriel Moura Mascarin.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Corrêa, B., da Silveira Duarte, V., Silva, D.M. et al. Comparative analysis of blastospore production and virulence of Beauveria bassiana and Cordyceps fumosorosea against soybean pests. BioControl (2020). https://doi.org/10.1007/s10526-020-09999-6

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Keywords

  • Biological control
  • Submerged fermentation
  • Hypocreales
  • Aleyrodidae
  • Noctuidae
  • Glycine max