, Volume 64, Issue 2, pp 173–183 | Cite as

Development of ready-to-use products derived from Bacillus subtilis strain CMs026 for plant disease control

  • Yotsanan WeerapolEmail author
  • Harit Nimraksa
  • Ampaiwan Paradornuwat
  • Pornsak Sriamornsak


Bacillus subtilis CMs026 is used for plant disease control. However, use of biocontrol for crops is limited by variable performances of control agents. Therefore, this study aimed to develop a ready-to-use, bacteria-based, highly stable, solid dosage formulation for plant disease control. Incubated broths containing B. subtilis CMs026 were centrifuged and sterilized. Supernatants were mixed with silicon dioxides and dried at 80 °C for 48 h. Agar diffusion test using active antifungal substance, surfactin, as a marker revealed the efficacy of dried broth. Subsequently, products were prepared by mixing dried broths with 6% disintegrant, followed by compaction. The compressed tablets were stable after storage for three months. In vivo experiments against Alternaria brassicicola in Chinese kale (Brassica alboglabra) revealed that the products reduced percent crop loss from 88% to 40%.


Bacteria Plant pathogen Biocontrol Bacillus subtilis 



This research was financially supported by The Thailand Research Fund (Division 2: Agriculture), Grant Number RGD5120080. The authors would like to thank Prof. Dr. Nuntavan Bunyapraphatsara for her valuable suggestions and comments on the research topic.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.


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Copyright information

© International Organization for Biological Control (IOBC) 2019

Authors and Affiliations

  1. 1.Faculty of Pharmaceutical SciencesBurapha UniversityChonburiThailand
  2. 2.Pharmaceutical Biopolymer Group (PBiG), Faculty of PharmacySilpakorn UniversityNakhon PathomThailand
  3. 3.Department of Agricultural Technology, Faculty of ScienceRamkhamhaeng UniversityBangkokThailand
  4. 4.Department of Plant Pathology, Faculty of AgricultureKasetsart UniversityBangkokThailand
  5. 5.Department of Pharmaceutical Technology, Faculty of PharmacySilpakorn UniversityNakhon PathomThailand
  6. 6.Academy of ScienceRoyal Society of ThailandBangkokThailand

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