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Bacillus sp. strain M10 as a potential biocontrol agent protecting chili pepper and tomato fruits from anthracnose disease caused by Colletotrichum capsici

  • Prapasri Srikhong
  • Kongyuth Lertmongkonthum
  • Rapeewan Sowanpreecha
  • Panan Rerngsamran
Article
  • 15 Downloads

Abstract

Bacillus sp. strain M10 was observed to produce an antifungal protein that inhibits the growth of Colletotrichum capsici, which is the causal agent of anthracnose disease of chili pepper and tomato. Ammonium sulfate precipitation, anion exchange chromatography, and sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed that the protein was approximately 55.4 kDa. The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and a subsequent sequence database search indicated the antifungal protein was most similar to the Bacillus amyloliquefaciens vegetative catalase (KatA) protein. Light microscopy observation revealed that the antifungal protein induced abnormal hyphal elongation and conidial swelling and rupture. The protein considerably inhibited anthracnose development and protected the fruits from C. capsici infection. Thus, Bacillus sp. strain M10 and/or its putative catalase may be useful as a post-harvest biocontrol agent that protects chili pepper and tomato fruits from anthracnose disease caused by C. capsici.

Keywords

Antifungal Bacillus Catalase Chili Colletotrichum Tomato 

Notes

Acknowledgement

This work was supported by The 90th Anniversary of Chulalongkorn University Fund (Ratchadaphiseksomphot Endowment Fund).

References

  1. Alina SO, Constantinscu F, Petruta CC (2015) Biodiversity of Bacillus subtilis group and beneficial traits of Bacillus species useful in plant protection. Rom Biotech Lett 20:10737–10750Google Scholar
  2. Alvindia DG, Acda MA (2015) The antagonistic effect and mechanisms of Bacillus amyloliquefaciens DGA14 against anthracnose in mango cv. ‘Carabao’. Biocontrol Sci Technol 25:560–572CrossRefGoogle Scholar
  3. Avis TJ (2007) Antifungal compounds that target fungal membranes: applications in plant disease control. Can J Plant Pathol 29:323–329CrossRefGoogle Scholar
  4. Barefoot SF, Klaenhammer TR (1983) Detection and activity of lactacin B, a bacteriocin produced by Lactobacillus acidophilus. Appl Environ Microbiol 45:1808–1815PubMedPubMedCentralGoogle Scholar
  5. Byrne JM, Hausbeck MK, Hammerschmidt R (1997) Conidial germination and appressorium formation of Colletotrichum coccodes on tomato foliage. Plant Dis 81:715–718CrossRefGoogle Scholar
  6. Chowdhury SP, Hartmann A, Gao X, Borriss R (2015) Biocontrol mechanism by root-associated Bacillus amyloliquefaciens FZB42—a review. Front Microbiol 6:780.  https://doi.org/10.3389/fmicb.2015.00780 CrossRefPubMedPubMedCentralGoogle Scholar
  7. Diao YZ, Zhang C, Xu JP, Lin D, Liu L, Mtung’e OG, Liu XL (2015) Genetic differentiation and recombination among geographic populations of the fungal pathogen Colletotrichum truncatum from chili peppers in China. Evol Appl 8:108–118CrossRefPubMedGoogle Scholar
  8. Dieguez-Uribeondo J, Forster H, Soto-Estrada A, Adaskaveg JE (2005) Subcuticular-intracellular hemibiotrophic and intercellular necrotrophic development of Colletotrichum acutatum on almond. Phytopathology 95:751–758CrossRefPubMedGoogle Scholar
  9. During K, Porsch P, Mahn A, Brinkmann O, Gieffers W (1999) The non-enzymatic microbicidal activity of lysozymes. FEBS Lett 449:93–100CrossRefPubMedGoogle Scholar
  10. El-Awady M, Moghaieb EEA, Haggag W, Sawsan Youssef S, El-Sharkawy AM (2008) Transgenic canola plants over-expressing bacterial catalase exhibit enhanced resistance to Peronospora parasitica and Erysiphe polygoni. Arab J Biotechnol 11:71–84Google Scholar
  11. Hou X, Boyetchko SM, Brkic M, Olson D, Ross A, Hegedus D (2006) Characterization of the anti-fungal activity of a Bacillus spp. associated with sclerotia from Sclerotinia sclerotiorum. Appl Microbiol Biotechnol 72:644–653CrossRefPubMedGoogle Scholar
  12. Kim BS, Hwang BK (2007) Microbial fungicides in the control of plant diseases. J Phytopathol 155:641–653CrossRefGoogle Scholar
  13. Kim PI, Ryu J, Kim YH, Chi YT (2010) Production of biosurfactant lipopeptides iturin A, fengycin and surfactin A from Bacillus subtilis CMB32 for control of Colletotrichum gloeosporioides. J Microbiol Biotechnol 20:138–145PubMedGoogle Scholar
  14. Kim JD, Jeon BJ, Han JW, Park MY, Kang SA, Kim BS (2016) Evaluation of the endophytic nature of Bacillus amyloliquefaciens strain GYL4 and its efficacy in the control of anthracnose. Pest Manag Sci 72:1529–1536CrossRefPubMedGoogle Scholar
  15. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685CrossRefPubMedGoogle Scholar
  16. Lavermicocca P, Lonigro SL, Evidente A, Andolfi A (1999) Bacteriocin production by Pseudomonas syringae pv. ciccaronei NCPPB2355. Isolation and partial characterization of the antimicrobial compound. J Appl Microbiol 86:257–265CrossRefGoogle Scholar
  17. Liao CY, Chen MY, Chen YK, Kuo KC, Chung KR, Lee MH (2012) Formation of highly branched hyphae by Colletotrichum acutatum within the fruit cuticles of Capsicum spp. Plant Pathol 61:262–270CrossRefGoogle Scholar
  18. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275PubMedGoogle Scholar
  19. Mataragas M, Metaxopoulos J, Galiotou M, Drosinos EH (2003) Influence of pH and temperature on growth and bacteriocin production by Leuconostoc mesenteroides L124 and Lactobacillus curvatus L442. Meat Sci 64:265–271CrossRefPubMedGoogle Scholar
  20. McLean KS, Roy KW (1991) Weeds as a source of Colletotrichum capsici causing anthracnose on tomato fruit and cotton seedlings. Can J Plant Pathol 13:131–134CrossRefGoogle Scholar
  21. Meerak J, Lida H, Watanabe Y, Miyashita M, Sato H, Nakagawa Y, Tahara Y (2007) Phylogeny of gamma-polyglutamic acid-producing Bacillus strains isolated from fermented soybean foods manufactured in Asian countries. J Gen Appl Microbiol 53:315–323CrossRefPubMedGoogle Scholar
  22. Nagorska K, Bikowski M, Obuchowski M (2007) Multicellular behaviour and production of a wide variety of toxic substances support usage of Bacillus subtilis as a powerful biocontrol agent. Acta Biochim Pol 54:495–508PubMedGoogle Scholar
  23. Ni J, Tokuyama S, Sogabe A, Kawamura Y, Tahara Y (2001) Cloning and high expression of catalase gene from Bacillus sp. TE124. J Biosci Bioeng 91:422–424CrossRefPubMedGoogle Scholar
  24. Rais A, Jabeen Z, Shair F, Hafeez FY, Hassan MN (2017) Bacillus spp., a bio-control agent enhances the activity of antioxidant defense enzymes in rice against Pyricularia oryzae. PLoS ONE 12(11):e0187412CrossRefPubMedPubMedCentralGoogle Scholar
  25. Selitrennikoff CP (2001) Antifungal proteins. Appl Environ Microbiol 67:2883–2894CrossRefPubMedPubMedCentralGoogle Scholar
  26. Shafi J, Tian H, Ji MS (2017) Bacillus species as versatile weapons for plant pathogens: a review. Biotechnol Biotec Eq 31:446–459CrossRefGoogle Scholar
  27. Sharma RR, Singh D, Singh R (2009) Biological control of postharvest diseases of fruits and vegetables by microbial antagonists: a review. Biol Control 50:205–221CrossRefGoogle Scholar
  28. Shuqing L, Nan Z, Zhenhua Z, Jia L, Biao S, Ruifu Z, Qirong S (2013) Antagonist Bacillus subtilis HJ5 controls Verticillium wilt of cotton by root colonization and biofilm formation. Biol Fert Soils 49:295–303CrossRefGoogle Scholar
  29. Wang LT, Lee FL, Tai CJ, Kasai H (2007) Comparison of gyrB gene sequences, 16S rRNA gene sequences and DNA-DNA hybridization in the Bacillus subtilis group. Int J Syst Evol Microbiol 57:1846–1850CrossRefPubMedGoogle Scholar
  30. Wang N, Liu M, Guo L, Yang X, Qiu D (2016) A novel protein elicitor (PeBA1) from Bacillus amyloliquefaciens NC6 induces systemic resistance in tobacco. Int J Biol Sci 12:757–767CrossRefPubMedPubMedCentralGoogle Scholar
  31. Xiao ZJ, Lu JR, Ma CQ, Xu P (2009) Formation and identification of trimethylimidazole during tetramethylpyrazine production from glucose by Bacillus strains. Biotechnol Lett 31:1421–1425CrossRefPubMedGoogle Scholar
  32. Yamamoto S, Shiraishi S, Suzuki S (2015) Are cyclic lipopeptides produced by Bacillus amyloliquefaciens S13-3 responsible for the plant defence response in strawberry against Colletotrichum gloeosporioides? Lett Appl Microbiol 60:379–386CrossRefPubMedGoogle Scholar

Copyright information

© International Organization for Biological Control (IOBC) 2018

Authors and Affiliations

  • Prapasri Srikhong
    • 1
  • Kongyuth Lertmongkonthum
    • 1
  • Rapeewan Sowanpreecha
    • 1
  • Panan Rerngsamran
    • 1
  1. 1.Department of Microbiology, Faculty of ScienceChulalongkorn UniversityBangkokThailand

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