BioControl

, Volume 60, Issue 5, pp 681–689

Impact of temperature on the survival and the biocontrol efficacy of Lysobacter capsici AZ78 against Phytophthora infestans

  • Gerardo Puopolo
  • Maria Cristina Palmieri
  • Oscar Giovannini
  • Ilaria Pertot
Article

Abstract

Temperature can strongly influence the biocontrol efficacy of Lysobacter strains when used against phytopathogens. We investigated the influence of temperature on physiological characteristics of Lysobacter capsici AZ78 in a biocontrol situation. L. capsici AZ78 effectively colonized tomato leaves at 15 and 25 °C while exposure to 35 and 37 °C was highly detrimental. Accordingly, Phytophthora infestans attacks on tomato leaves were controlled when L. capsici AZ78 was pre-exposed to 15 and 25 °C for 24 h. Conversely, exposure to 35 °C drastically reduced the biocontrol efficacy of L. capsici AZ78 probably by impairing anti-oomycete and proteolytic activity. Furthermore, the exposure to 15 °C made the L. capsici AZ78 cells more resistant to UV light and copper ions compared to 25 and 35 °C. These findings give an indication on the best temperature range for the future use of biofungicides based on L. capsici AZ78 cells.

Keywords

Biological control Temperature Lysobacter capsici Phytophthora infestans 

Supplementary material

10526_2015_9672_MOESM1_ESM.docx (175 kb)
Fig. S1 Effect of temperature on in vitro growth of Lysobacter capsici AZ78 in liquid medium. AZ78 cell growth was monitored over 144 h by measuring optical density at 600 nm (OD600nm) spectrophotometrically. Points indicate mean ± SE (n = 6). Supplementary material 1 (DOCX 174 kb)
10526_2015_9672_MOESM2_ESM.docx (15 kb)
Table S1 Presence of viable Lysobacter capsici AZ78 cells on the leaves of tomato plants exposed to different temperatures. Colonisation of tomato leaves by AZ78 was monitored one day (T1) and eight days (T2) after its application. AZ78 populations are expressed as log10 CFU g−1 of leaf. Values (mean values ± SE, n = 12) with different letters are significantly different between treatments according to Tukey’s test (P < 0.05). Supplementary material 2 (DOCX 15 kb)

Copyright information

© International Organization for Biological Control (IOBC) 2015

Authors and Affiliations

  1. 1.Department of Sustainable Agro-Ecosystems and Bioresources, Research and Innovation CentreFondazione Edmund Mach (FEM)S. Michele all’AdigeItaly

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