Impact of temperature on the survival and the biocontrol efficacy of Lysobacter capsici AZ78 against Phytophthora infestans
- 227 Downloads
Temperature can strongly influence the biocontrol efficacy of Lysobacter strains when used against phytopathogens. We investigated the influence of temperature on physiological characteristics of Lysobacter capsici AZ78 in a biocontrol situation. L. capsici AZ78 effectively colonized tomato leaves at 15 and 25 °C while exposure to 35 and 37 °C was highly detrimental. Accordingly, Phytophthora infestans attacks on tomato leaves were controlled when L. capsici AZ78 was pre-exposed to 15 and 25 °C for 24 h. Conversely, exposure to 35 °C drastically reduced the biocontrol efficacy of L. capsici AZ78 probably by impairing anti-oomycete and proteolytic activity. Furthermore, the exposure to 15 °C made the L. capsici AZ78 cells more resistant to UV light and copper ions compared to 25 and 35 °C. These findings give an indication on the best temperature range for the future use of biofungicides based on L. capsici AZ78 cells.
KeywordsBiological control Temperature Lysobacter capsici Phytophthora infestans
The authors wish to thank Denise Ress for her technical assistance and Dr. Valerio Mazzoni for discussion on statistical analysis. This research was supported by the EU project CO-FREE (theme KBBE.2011.1.2-06, Grant agreement number 289497).
- Compant S, van der Heijden MGA, Sessitsch A (2010) Climate change effects on beneficial plant-microrganism interactions. Environ Microbiol 73:197–214Google Scholar
- Elad Y, Pertot I (2012) Climate change impact on plant pathogens and plant disease. In: Manjit S. Kang (ed) Combating climate change: an agricultural perspective, CRC Press (Taylor & Francis Group), Boca Raton, FL, 183–212Google Scholar
- Ingraham J (1987) Effect of temperature, pH, water activity, and pressure on growth. In: Neidhardt FC, Ingraham JL, Low KB, Magasanik B, Schaechter M, Umbarger HE (eds) Escherichia coli and Salmonella: cellular and molecular biology. American Society for Microbiology, Washington, DC, pp 1543–1554Google Scholar
- IPCC-Intergovernmental Panel on Climate Change (2014) Cimate Change. Fifth assessment synthesis report. Summary for policy makers. Available at http://www.ipcc.ch
- Nicot PC, Alabouvette C, Bardin M, Blum B, Kohl J, Ruocco M (2012) Review of factors influencing the success or failure of biocontrol: technical, industrial and socio-economic perspectives. IOBC/WPRS Bull 78:95–98Google Scholar
- Puopolo G, Cimmino A, Palmieri MC, Giovannini O, Evidente A, Pertot I (2014b) Lysobacter capsici AZ78 produces cyclo(L-Pro- L-Tyr), a 2,5-diketopiperazine with toxic activity against sporangia of Phytophthora infestans and Plasmopara viticola. J Appl Microbiol 117:1168–1180CrossRefPubMedGoogle Scholar
- Schmidt CS, Agostini F, Leifert C, Killham K, Mullins CE (2004) Influence of soil temperature and matric potential on sugar beet seedling colonization and suppression of Pythium damping-off by the antagonistic bacteria Pseudomonas fluorescens and Bacillus subtilis. Phytopathology 94:351–363CrossRefPubMedGoogle Scholar