Cold storage of the predatory mite Neoseiulus californicus is improved by pre-storage feeding on the diapausing spider mite Tetranychus urticae
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Low air temperature accompanied with high humidity is effective for long-term cold storage of the predatory mite Neoseiulus californicus (McGregor) (Acari: Phytoseiidae). To further improve this storage method, we investigated the effect of pre-storage nutrition on survival during storage and on post-storage quality in terms of survival, oviposition, and progeny viability. The predatory mite was fed from the egg to adult stage on the diapausing two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), non-diapausing spider mites, or Japanese pear pollen, Pyrus pyrifolia Nakai. Newly emerged N. californicus adult females and males were mated, and then both were stored at 7.5 °C and a vapor pressure deficit of 0.0 kPa for up to 75 days. Survival during storage and post-storage quality was significantly better with the diapausing spider mite diet than with the other diets. No effects on the survival or sex ratio of the progeny of the stored adults were observed, regardless of diet or storage duration. Providing diapausing spider mites as a pre-storage diet therefore significantly improves the long-term storage of N. californicus. We discuss the possibility that ingestion of the cryoprotectants, antioxidants, and energy reserves that are present in rich amounts in diapausing spider mites mitigates chilling injury.
KeywordsCryoprotectants Natural enemies Oviposition Pollen Survival Vapor pressure deficit
Authors are indebted to Prof. MH. Osakabe of Kyoto University for helpful discussion. We also thank anonymous reviewers for their helpful suggestions. This study was supported by Grants-in-Aid for JSPS Fellows [22-2650 and 25-03084].
- Chang YF, Tauber MJ, Tauber CA (1995) Storage of the mass-produced predator Chrysoperla carnea (Neuroptera: Chrysopidae): influence of photoperiod, temperature, and diet. Environ Entomol 24:1365–1374Google Scholar
- Fields PG, Fleurat-Lassard F, Lavenseau L, Febvay G, Peypelut L, Bonnot G (1998) The effect of cold acclimation and deacclimation on cold tolerance, trehalose and free amino acid levels in Sitophilus granarius and Cryptolestes ferrugineus (Coleoptera). J Insect Physiol 44:955–965CrossRefPubMedGoogle Scholar
- Lee RE Jr (1991) Principles of insect low temperature tolerance. In: Lee RE Jr, Denlinger DL (eds) Insects at low temperature. Chapman & Hall, New York, USA, pp 17–46Google Scholar
- Leopold RA (1998) Cold storage of insects for integrated pest management. In: Hallman GJ, Denlinger DL (eds) Temperature sensitivity in insects and application in integrated pest management. Westview Press, Boulder, USA, pp 235–267Google Scholar
- Somme L (1999) The physiology of cold hardiness in terrestrial arthropods. Eur J Entomol 96:1–10Google Scholar
- Tauber MJ, Tauber CA, Nechols JR, Obrycki JJ (1983) Seasonal activity of parasitoids: control by external, internal and genetic factors. In: Brown VK, Hodek I (eds) Diapause and life cycle strategies in insects. Dr W Junk Publishers, The Hague, The Netherlands, pp 87–108Google Scholar
- Tauber MJ, Tauber CA, Masaki S (1986) Seasonal adaptations of insects. Oxford University Press, Oxford, UKGoogle Scholar
- Veerman A (1985) Diapause. In: Helle W, Sabelis MW (eds) Spider mites: their biology, natural enemies and control, vol 1A. Elsevier, Amsterdam, The Netherlands, pp 279–316Google Scholar
- Yoder JA, Benoit JB, Denlinger DL, Rivers DB (2006) Stress-induced accumulation of glycerol in the flesh fly, Sarcophaga bullata: evidence indicating anti-desiccant and cryoprotectant functions of this polyol and a role for the brain in coordinating the response. J Insect Physiol 52:202–214CrossRefPubMedGoogle Scholar