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BioControl

, Volume 57, Issue 6, pp 827–836 | Cite as

In vitro and in vivo evaluation of microbial-enriched compost tea on the development of powdery mildew on melon

  • Yuvarani Naidu
  • Sariah Meon
  • Yasmeen Siddiqui
Article

Abstract

This study evaluated the effects of microbial-enriched compost tea (CT) on the conidial germination of Golovinomyces cichoracearum DC. and development of powdery mildew on melons in a time-dependent manner. In vitro conidial germination was significantly reduced by 94 % and 85 % upon treatment with Daconil® (fungicide) or microbial-enriched CT, respectively, 96 h after incubation (hai). Morphological analysis under light microscopy demonstrated that conidia co-incubated with microbial-enriched CT at 48 hai appeared ruptured, which contributed to higher inhibition of conidial germination, increased cell permeability and leakage of cellular contents. These observations may be explained by antibiosis. Moreover, different application time of microbial-enriched CT on melons significantly affected disease development. There was a delay in disease development by 12 days in plants treated with Daconil®, microbial-enriched CT applied 24 h after inoculation and microbial-enriched CT applied simultaneously with inoculation when compared to the control treatment. Curative application of microbial-enriched CT (24 h after inoculation) delayed the onset of disease, and the efficiency of inhibition was comparable to a fungicidal spray (Daconil®). Hence, microbial-enriched CT may be used to inhibit the development of powdery mildew on melons, thus reducing the dependency on chemical fertilisers.

Keywords

Compost tea Conidial germination Golovinomyces cichoracearum DC. Curative application Biological control 

Notes

Acknowledgments

The authors wished to thank the Ministry of Science, Technology and Innovation (MOSTI) for the research grant received with Project No : 05-01-04- SF0181 under which this work was initiated.

References

  1. Al-Dahmani JH, Abbasi PA, Miller SA, Hoitink HAJ (2003) Suppression of bacterial spot of tomato with foliar sprays of compost tea under greenhouse and field conditions. Plant Dis 87:913–919CrossRefGoogle Scholar
  2. Al-Mughrabi KI (2007) Suppression of Phytophthora infestans in potatoes by foliar application of food nutrients and compost tea. Aust J Basic Appl Sci 1:785–792Google Scholar
  3. Al-Mughrabi KI, Berthélémé C, Livingston T, Burgoyne A, Poirier R, Vikram A (2008) Aerobic compost tea, compost and a combination of both reduce the severity of common scab (Streptomyces scabiei) on potato tubers. J Plant Sci 3:168–175CrossRefGoogle Scholar
  4. Baker KF, Cook RJ (1982) Biological control of plant pathogens. The American Phytopathological Society, St Paul, USA, p 433Google Scholar
  5. Campbell CL, Madden LV (1990) Introduction to plant disease epidemiology. Wiley-Interscience, New York, USAGoogle Scholar
  6. Chaube HM, Pundhir VS (2005) Disease management and forecast. In: Rangaswami G (ed) Crop diseases and their management. Prentice Hall of India, New Delhi, India, pp 171–189Google Scholar
  7. Cronin MJ, Yohalem DS, Harris RF, Andrews JH (1996) Putative mechanism and dynamics of inhibition of the apple scab pathogen (Venturia inequalis) by compost tea. Soil Biol Biochem 28:1241–1249CrossRefGoogle Scholar
  8. El- Masry M, Khalil AI, Hassouna MS, Ibrahim HAH (2002) In situ and in vitro suppressive effect of agricultural composts and their water extracts on some phytopathogenic fungi. World J Microbiol Biotechnol 18:551–558CrossRefGoogle Scholar
  9. Hoitink HA, Stone AG, Han DY (1997) Suppression of plant diseases by composts. HortScience 32:184–187Google Scholar
  10. Hoitink HAJ, Krause MS, Han DY (2001) Spectrum and mechanisms of plant disease control with composts. In: Stoffella PJ, Kahn BA (eds) Compost utilization in horticultural cropping systems. Lewis Publishers, Boca Raton, USA, pp 263–274Google Scholar
  11. Ingham ER (2000) The compost tea brewing manual. Unisun Communications, Corvallis, USAGoogle Scholar
  12. Koné SB, Dionne A, Tweddell RJ, Antoun H, Avis TJ (2010) Suppressive effect of non-aerated compost teas on foliar fungal pathogens of tomato. Biol Control 52:167–173CrossRefGoogle Scholar
  13. Kuzuya M, Yashiro K, Tomita K, Ezura H (2006) Powdery mildew (Podosphaera xanthii) resistance in melon is categorized into two types based on inhibition of the infection processes. J Exp Bot 57:2093–2100PubMedCrossRefGoogle Scholar
  14. Li YH, Windham MT, Trigiano RN, Fare DC, Spiers JM, Copes WE (2005) Spore germination, infection structure formation, and colony development of Erysiphe pulchra on dogwood leaves and glass slides. Plant Dis 89:1301–1304CrossRefGoogle Scholar
  15. Litterick AM, Harrier L, Wallace P, Watson CA, Wood M (2004) The role of uncomposted materials, composts, manures, and compost tea in reducing pest and disease incidence and severity in sustainable temperate agricultural and horticultural crop production—a review. Crit Rev Plant Sci 23:453–480CrossRefGoogle Scholar
  16. McGrath MT (2001) Fungicide resistance in cucurbit powdery mildew: expertise and challenges. Plant Dis 85:236–245CrossRefGoogle Scholar
  17. McQuilken MP, Whipps JM, Lynch JM (1994) Effects of water extract of a composted manure-straw mixture on the plant pathogen Botrytis cinerea. World J Microbiol Biotechnol 10:20–26CrossRefGoogle Scholar
  18. Naidu Y, Sariah M, Jugah K, Siddiqui Y (2010) Microbial starter for the enhancement of biological activity of compos tea. Int J Agric Biol 12:51–56Google Scholar
  19. Romero D, Rivera ME, Cazorla FM, de Vicente A, Pérez-García A (2003) Effect of mycoparasitic fungi on the development of Sphaerotheca fusca in melon leaves. Mycol Res 107:64–71PubMedCrossRefGoogle Scholar
  20. Romero D, de Vicente A, Olmos JL, Dávila JC, Pérez-García A (2007) Effect of lipopeptides of antagonistic strains of Bacillus subtilis on the morphology and ultrastructure of the cucurbit fungal pathogen Podosphaera fusca. J Appl Microbiol 103:969–976PubMedCrossRefGoogle Scholar
  21. Sang MK, Kim KD (2011) Biocontrol activity and primed systemic resistance by compost water extracts against anthracnoses of pepper and cucumber. Phytopathology 101:732–740PubMedCrossRefGoogle Scholar
  22. Scheuerell SJ, Mahaffee WF (2000) Assessing aerated and non-aerated watery fermented compost and Trichoderma harzianum T-22 for control of powdery mildew of rose in the Willamette Valley, Oregon. Phytopathology 90:S69Google Scholar
  23. Scheuerell SJ, Mahaffee WF (2004) Compost tea as a container medium drench for suppressing seedling damping-off caused by Pythium ultimum. Phytopathology 94:1156–1163PubMedCrossRefGoogle Scholar
  24. Scheuerell SJ, Mahaffee WF (2006) Variability associated with suppression of gray mold (Botrytis cinerea) on geranium by foliar application of non-aerated and aerated compost teas. Plant Dis 90:1201–1208CrossRefGoogle Scholar
  25. Segarra G, Reis M, Casanova E, Trillas MI (2009) Control of powdery mildew (Erysiphe Polygoni) in tomato by foliar applications of compost tea. J Plant Pathol 91:683–689Google Scholar
  26. Shrestha K, Shrestha P, Walsh KB, Harrower KM, Midmore DJ (2011) Microbial enhancement of compost extracts based on cattle rumen content compost—characterisation of a system. Bioresour Technol 102:8027–8034PubMedCrossRefGoogle Scholar
  27. Siddiqui Y, Sariah M, Razi I (2008) Trichoderma-fortified compost extracts for the control of Choanephora wet rot in okra production. Crop Prot 27:385–390CrossRefGoogle Scholar
  28. Siddiqui Y, Sariah M, Razi I, Mawardi R (2009) Bio-potential of compost tea from agro-waste to suppress Choanephora cucurbitarum L. the causal pathogen of wet rot of okra. Biol Control 49:38–44CrossRefGoogle Scholar
  29. Stindt A, Weltzien HC (1990) Untersuchungen zur Wirkung und zu den Wirkungs-mechanismen von Kompost Extrackten auf Botrytis cinerea Pers. Ex. Nocca & Balb an Erdbeeren. Kopfsalat und Buschboh-nen. Erwerbsobstbau 33:28–29Google Scholar
  30. Welke SE (2004) The effect of compost tea on the yield of strawberries and the severity of Botrytis cinerea. J Sustain Agric 25:57–68CrossRefGoogle Scholar
  31. Zhang W, Han DY, Dick WA, Davis KR, Hoitink HAJ (1998) Compost and compost water extract-induced systemic acquired resistance in cucumber and Arabidopsis. Phytopathology 88:450–455PubMedCrossRefGoogle Scholar

Copyright information

© International Organization for Biological Control (IOBC) 2012

Authors and Affiliations

  • Yuvarani Naidu
    • 1
    • 2
  • Sariah Meon
    • 1
  • Yasmeen Siddiqui
    • 1
  1. 1.Laboratory of Food Crops and Floriculture, Institute of Tropical AgricultureUniversiti Putra MalaysiaSerdangMalaysia
  2. 2.Malaysian Palm Oil BoardKajangMalaysia

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