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BioControl

, Volume 56, Issue 5, pp 781–788 | Cite as

Lethal and sublethal effects of Iranian isolates of Steinernema feltiae and Heterorhabditis bacteriophora on the Colorado potato beetle, Leptinotarsa decemlineata

  • L. Ebrahimi
  • G. NiknamEmail author
  • E. E. Lewis
Article

Abstract

To determine the LC50 values of two entomopathogenic nematodes against Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae) prepupae, different concentrations of the nematodes were tested in soil. Because of the different temperature requirements of the two nematode species, bioassay experiments were conducted at 20 ± 1°C and 27 ± 2°C for Steinernema feltiae Filipjev (Rhabditida: Steinernematidae) and Heterorhabditis bacteriophora Poinar (Rhabditida: Heterorhabditidae), respectively. Both the isolates were effective against L. decemlineata. LC50 values of H. bacteriophora against progeny of field-collected adults and laboratory-reared adults were estimated as 8.5 and 7.6 IJ per prepupa, respectively. For S. feltiae the value was calculated as 51.2 IJ per prepupa against offspring of laboratory-reared adults of L. decemlineata only. Cellular encapsulation of both nematode species was observed. Sublethal nematode concentrations caused wing deformation and delayed metamorphosis which may affect Colorado potato beetle adult fitness.

Keywords

Chrysomelidae Insect fitness Heterorhabditidae LC50 Steinernematidae Prepupa 

References

  1. Adams BJ, Nguyen KB (2002) Taxonomy and systematics. In: Gaugler R (ed) Entomopathogenic nematology. CABI Publishing, Wallingford, pp 1–34CrossRefGoogle Scholar
  2. Armer CA, Berry RE, Reed GL, Jepsen SJ (2004a) Colorado potato beetle control by application of the entomopathogenic nematode Heterorhabditis marelata and potato plant alkaloid manipulation. Entomol Exp Appl 111:47–58CrossRefGoogle Scholar
  3. Armer CA, Rao S, Berry RE (2004b) Insect cellular and chemical limitations to pathogen development: the Colorado potato beetle, the nematode Heterorhabditis marelatus, and its symbiotic bacteria. J Invertebr Pathol 87:114–122CrossRefPubMedGoogle Scholar
  4. Bedding RA, Molyneux AS, Akhurst RJ (1983) Heterorhabditis spp., Neoaplectana spp., and Steinernema kraussei: Interspecific and intraspecific differences in infectivity for insects. Exp Parasitol 55:249–257CrossRefPubMedGoogle Scholar
  5. Berry RE, Liu J, Reed G (1997) Comparison of endemic and exotic entomopathogenic nematode species for control of Colorado potato beetle (Coleoptera: Chrysomelidae). J Econ Entomol 90:1528–1533CrossRefPubMedGoogle Scholar
  6. Blackburn MB, Domek JM, Gelman DB, Hu JS (2005) The broadly insecticidal Photorhabdus luminescens toxin complex (Tca): activity against the Colorado potato beetle, Leptinotarsa decemlineata, and sweet potato whitefly, Bemisia tabaci. J Insect Sci 35:1–11Google Scholar
  7. Blackburn M, Farrar R, Gundersen-Rindal D, Lawrence S, Martin Ph (2007) Reproductive failure of Heterorhabditis marelata in the Colorado potato beetle: evidence of stress on the nematode symbiotic Photorhabdus temperata, and potential interference from the enteric bacteria of the beetle. Biol Control 42:207–215CrossRefGoogle Scholar
  8. Blackburn MB, Gundersen-Rindal DE, Weber DC, Martin Ph, Farrar RR (2008) Enteric bacteria of field-collected Colorado potato beetle larvae inhibit growth of the entomopathogens Photorhabdus temperata and Beauveria bassiana. Biol Control 46:434–441CrossRefGoogle Scholar
  9. Burnell AM, Stock SP (2000) Heterorhabditis, Steinernema and their bacterial symbiont- lethal pathogens of insects. Nematology 2:31–42CrossRefGoogle Scholar
  10. Cantelo WW, Nickle WR (1992) Susceptibility of prepupae of the Colorado potato beetle (Coleoptera: Chrysomelidae) to entomopathogenic nematodes (Rhabditida: Steinernematidae, Heterorhabditidae). J Entomol Sci. 27:37–43Google Scholar
  11. Dowds BCA, Peters A (2002) Virulence mechanisms. In: Gaugler R (ed) Entomopathogenic nematology. CABI Publishing, Wallingford, pp 79–98CrossRefGoogle Scholar
  12. Ebrahimi L, Niknam G, Eivazian N, Nikdel M (2008) Study on the life cycle of two entomopathogenic nematodes, Steinernema feltiae and Heterorhabditis bacteriophora at four various temperatures. In: 18th Iranian Plant Protection Congress Proceedings, Pests, vol I. p 6Google Scholar
  13. Ferro DN, Morzuch BJ, Margolies D (1983) Crop loss assessment of the Colorado potato beetle (Coleoptera: Chrysomelidae) on potatoes in western Massachusetts. J Econ Entomol 76:349–356CrossRefGoogle Scholar
  14. French NM II, Heim DC, Kennedy GG (1992) Insecticide resistance patterns among Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) populations in North Carolina. Pestic Sci 36:95–100CrossRefGoogle Scholar
  15. Hitchner EM (2007) Investigations of the integrated pest management of Colorado potato beetle, Leptinotarsa decemlineata (Say): host plant preference, development of semiochemical-based strategies, and evaluation of a novel insecticide. PhD thesis, Virginia UniversityGoogle Scholar
  16. Koppenhöfer AM (2007) Nematodes. In: Lacey LA, Kaya HK (eds) Field manual of techniques in invertebrate pathology application and evaluation of pathogens for control of insects and other invertebrate pests. Springer, Dordrecht, pp 249–266CrossRefGoogle Scholar
  17. Lazzaro BP, Little TJ (2009) Immunity in a variable world. Philos. Trans R Soc B 364:15–26CrossRefGoogle Scholar
  18. Mohammadi Sharif M, Hejazi MJ, Mohammadi A, Rashidi MR (2007) Resistance status of the Colorado potato beetle, Leptinotarsa decemlineata, to endosulfan in East Azarbaijan and Ardebil provinces of Iran. J Insect Sci 7:1–7CrossRefPubMedGoogle Scholar
  19. Nickle RW, Connick WJ Jr, Cantelo WW (1994) Effects of pesta-pelletized Steinernema carpocapsae (All) on western corn rootworm and Colorado potato beetle. J Nematol 26:249–250PubMedPubMedCentralGoogle Scholar
  20. Nouri Ganbalani G (1986) Colorado potato beetle (in Persian). University of Tabriz Publications, TabrizGoogle Scholar
  21. Pedigo LP (2004) Entomology and pest management, 4th edn. Prentice-Hall of India Private Limited, New DelhiGoogle Scholar
  22. Price PW (1997) Insect ecology. John Wiley & Sons, New YorkGoogle Scholar
  23. Robertson JL, Russell RM, Preisler HK, Savin NE (2007) Bioassays with arthropods, 2nd edn. CRC Press, Boca Raton, pp 21–34Google Scholar
  24. Salmanov AA (1988) Results of attempted infection of the Colorado potato beetle with the nematode Neoaplectana carpocapsae in laboratory and field conditions. Izvestiya Akademii Nauk Azerbaidzhanskoi SSr, Biologicheskie Nauki (Russian) 2:45–47Google Scholar
  25. Saringer G, Fodor A, Nadasy M, Lucskai A, Georgis R (1996) Possibilities of biological control using entomopathogenic nematodes against Leptinotarsa decemlineata L. and Athalia rosae L. larvae. Mededelingen- Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen, Universiteit Gent. 61:961–966. In: 48th International symposium on crop protection, PTSI-IV 61 (2A&B, 3A)Google Scholar
  26. SAS Institute (2004) SAS enterprise guide ver. 3.0Google Scholar
  27. Thurston GS, Yule WN, Dunphy GB (1994) Explanations for the low susceptibility of Leptinotarsa decamlineata to Steinernema carpocapsae. Biol Control 4:53–58CrossRefGoogle Scholar
  28. Toba HH, Lindegren JE, Turner JE, Vail PV (1983) Susceptibility of the Colorado potato beetle and the sugarbeet wireworm to Steinernema feltiae and S. glaseri. J Nematol 15:597–601PubMedPubMedCentralGoogle Scholar
  29. Woodring JL, Kaya HK (1988) Steinernematid and Heterorhabditid nematodes: a handbook of biology and techniques. Southern cooperative series bulletin 331. Arkansas Agricultural Experiment Station, FayettevilleGoogle Scholar
  30. Wright RJ, Agudelo-Silva F, Georgis R (1987) Soil applications of steinernematid and heterorhabditid nematodes for control of Colorado potato beetle, Leptinotarsa decemlineata (Say). J Nematol 19:201–206PubMedPubMedCentralGoogle Scholar

Copyright information

© International Organization for Biological Control (IOBC) 2011

Authors and Affiliations

  1. 1.Nematology Laboratory, Department of Plant Protection, Faculty of AgricultureUniversity of TabrizTabrizIran
  2. 2.Department of NematologyUniversity of CaliforniaDavisUSA

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