BioControl

, Volume 56, Issue 1, pp 101–112 | Cite as

Differential resistance of oilseed rape cultivars (Brassica napus ssp. oleifera) to Verticillium longisporum infection is affected by rhizosphere colonisation with antagonistic bacteria, Serratia plymuthica and Pseudomonas chlororaphis

Article

Abstract

The effect of a seed treatment with the antagonistic bacteria Serratia plymuthica (strain HRO-C48) and/or Pseudomonas chlororaphis (strain MA 342) on the infection of oilseed rape with Verticillium longisporum was assessed with ten different cultivars. Soil was inoculated with microsclerotia and mycelium of a V. longisporum culture. Seeds were treated with rifampicin-resistant antagonistic bacteria at a rate of log10 6–7 cells per seed. Resistance against V. longisporum infection did not differ between cultivars and was generally low. A significant disease reduction recorded as area under disease progress curve (AUDPC) was obtained with both antagonistic rhizobacteria with no significant difference between the treatments. Percent of healthy plants was approximately 70% in all bacterial treatments. Significant differences were observed between the cultivars ranging from 46.5% (cultivar Titan) to 72.6% (Trabant). The combined use of both bacteria could not provide additional control effects. The bacterial density in the rhizosphere was not related to the control effect, but increased by log10 2 on infection with V. longisporum. Growth promotion effects were also not related to the control effect. At present, neither the application of chemical fungicides nor breeding for resistance against V. longisporum in oilseed rape can provide a solution for this increasingly problematic plant pathogen. The present results now open perspectives to control V. longisporum in oilseed rape by making use of cultivars, which express resistance against this pathogen on interaction with the antagonistic rhizobacteria S. plymuthica or P. chlororaphis.

Keywords

Oilseed rape Verticillium longisporum Seed priming Antagonistic bacteria Root colonization Growth promotion Resistance 

Notes

Acknowledgment

The scholarship by the DAAD (Deutscher Akademischer Austauschdienst) to the first author is highly appreciated.

References

  1. Bautista G, Mendoza H, Uribe D (2007) Biocontrol of Rhizoctonia solani in native potato (Solanum phureja) plants using native Pseudomonas fluorescens. Acta Biol Colomb 12:19–32Google Scholar
  2. Berg G (1996) Rhizobacteria of oil seed rape antagonistic to Verticillium dahliae. J Plant Dis Prot 103:20–30Google Scholar
  3. Berg G (2000) Diversity of antifungal and plant-associated Serratia plymuthica strains. J Appl Microbiol 88:952–960CrossRefPubMedGoogle Scholar
  4. Berg G, Fritze A, Roskot N, Smalla K (2001) Evaluation of potential biocontrol rhizobacteria from different host plants of Verticillium dahliae Kleb. J Appl Microbiol 156:75–82Google Scholar
  5. Berg G, Roskot N, Steidle A, Eberl L, Zock A, Smalla K (2002) Plant-dependent genotypic and phenotypic diversity of antagonistic rhizobacteria isolated from different Verticillium host plants. Appl Environ Microbiol 68:3328–3338CrossRefPubMedGoogle Scholar
  6. Campbell CL, Madden LV (1990) Introduction to plant disease epidemiology. Wiley, New York, 532 ppGoogle Scholar
  7. Collins A, Okoli CAN, Morton A, Parry D, Edwards SG, Barbara DJ (2003) Isolates of Verticillium dahliae pathogenic to crucifers are of at least three distinct molecular types. Phytopathology 93:364–376CrossRefPubMedGoogle Scholar
  8. Compant S, Duffy B, Nowak J, Clement C, Barka EA (2005) Use of plant growth-promoting bacteria for biocontrol of plant diseases: principle, mechanisms of action, and future prospects. Appl Environ Microbiol 71:4951–4959CrossRefPubMedGoogle Scholar
  9. De Vleesschauwer D, Höfte M (2007) Using Serratia plymuthica to control fungal pathogens of plants. CAB Rev 46:1–12Google Scholar
  10. Debode J, Clewes E, De Backer G, Höfte M (2005) Lignin is involved in the reduction of Verticillium dahliae var. longisporum inoculum in soil crop residue incorporation. Soil Biol Biochem 37:301–309CrossRefGoogle Scholar
  11. Debode J, De Maeyer K, Perneel M, Pannecoucque J, De Backer G, Höfte M (2007) Biosurfactants are involved in the biological control of Verticillium microsclerotia by Pseudomonas spp. J Appl Microbiol 103:1184–1196CrossRefPubMedGoogle Scholar
  12. Eurostat (2007) Eurostat website and national data. http://epp.eurostat.ec.europa.eu
  13. Eynck C, Koopmann B, Grunewaldt-Stoeker G, Karlovsky P, von Tiedemann A (2007) Differential interactions of Verticillium longisporum and Verticillium dahliae with Brassica napus detected with molecular and histological techniques. Eur J Plant Pathol 118:259–274CrossRefGoogle Scholar
  14. Fitt BDL, Gladders P, Turner JA, Sutherland KG, Welham SJ, Davies JM (1997) Prospects for developing a forecasting scheme to optimise use of fungicides for disease control on winter rape in the UK. Asp Appl Biol 48:135–142Google Scholar
  15. Fitt BDL, Brun H, Barbetti MJ, Rimmer SR (2006) World-wide importance of Phoma stem canker (Leptosphaeria maculans and L. biglobosa) on oilseed rape (Brassica napus). Eur J Plant Pathol 114:3–15CrossRefGoogle Scholar
  16. Frankowski J, Lorito M, Schmid R, Berg G, Bahl H (2001) Purification and properties of two chitinolytic enzymes of Serratia plymuthica HRO-C48. Arch Microbiol 176:421–426CrossRefPubMedGoogle Scholar
  17. Hammoudi O (2007) Einfluss mikrobieller Antagonisten auf den Befall mit Phoma lingam und Verticillium dahliae var. longisporum an Raps (Brassica napus L. var. napus). Dissertation, Christian-Albrechts-University zu KielGoogle Scholar
  18. Heale JB, Karapapa VK (1999) The Verticillium threat to Canada’s major oilseed crop: canola. Can J Plant Pathol 21:1–7Google Scholar
  19. Hökeberg M (2006) Development and registration of biocontrol products—experience and perspectives gained from the bacterial seed treatment products Cedomon® and Cerall®. In: Proceedings of the international workshop “Implementation of biocontrol in practice in temperate regions—present and near future”, Research Centre Flakkebjerg, Denmark, November 1–3, 2005. DIAS Report 119, p 77Google Scholar
  20. Hökeberg M, Gerhardson B, Johnsson L (1997) Biological control of cereal seed-borne diseases by seed bacterization with greenhouse-selected bacteria. Eur J Plant Pathol 103:25–33CrossRefGoogle Scholar
  21. Johnsson L, Hökeberg M, Gerhardson B (1998) Performance of the Pseudomonas chlororaphis biocontrol agent MA 342 against cereal seed-borne diseases in field experiments. Eur J Plant Pathol 104:701–711CrossRefGoogle Scholar
  22. Kalbe C, Marten P, Berg G (1996) Members of the genus Serratia as beneficial rhizobacteria of oilseed rape. Microbiol Res 151:400–433Google Scholar
  23. Karapapa VK, Bainbridge BW, Heale JB (1997) Morphological and molecular characterization of Verticillium longisporum comb. nov., pathogenic to oilseed rape. Mycol Res 101:1281–1294CrossRefGoogle Scholar
  24. Khan A, Sutton JC, Grodzinski B (2003) Effect of Pseudomonas chlororaphis on Pythium aphanidermatum and root rot in peppers grown in small-scale hydroponic troughs. Biocontrol Sci Technol 13:615–630CrossRefGoogle Scholar
  25. Kurze S, Dahl R, Bahl H, Berg G (2001) Biological control of soil-borne pathogens in strawberry by Serratia plymuthica HRO-C48. Plant Dis 85:529–534CrossRefGoogle Scholar
  26. Lugtenberg BJJ, Chin-A-Woeng TFC, Bloemberg GV (2002) Microbe–plant interactions: principles and mechanisms. Antonie Van Leeuwenhoek 81:373–383CrossRefPubMedGoogle Scholar
  27. Mazzola M, Cook RJ (1991) Effects of fungal root pathogens on the population dynamics of biocontrol strains of fluorescent pseudomonads in the wheat rhizosphere. Appl Environ Microbiol 57:2171–2178PubMedGoogle Scholar
  28. Messner R, Schweigkofler W, Schweigkofler M, Berg G, Prillinger H (1996) Molecular characterization of the plant pathogen Verticillium dahliae Kleb. using RAPD-PCR and sequencing of the 18S rRNA-gene. J Phytopathol 144:347–354CrossRefGoogle Scholar
  29. Mol L, van Riessen HW (1995) Effect of plant-roots in the germination of microsclerotia of Verticillium dahliae. Eur J Plant Pathol 101:673–678CrossRefGoogle Scholar
  30. Müller H, Berg G (2008) Impact of formulation procedures on the effect of the biocontrol agent Serratia plymuthica HRO-C48 on Verticillium wilt in oilseed rape. BioControl 53:905–916CrossRefGoogle Scholar
  31. Osborne DJ, McManus T (2005) Hormones, signals and target cells in plant development. Cambridge University Press, CambridgeCrossRefGoogle Scholar
  32. Robb J (2007) Verticillium tolerance: resistance, susceptibility or mutualism. Can J Bot 85:903–910CrossRefGoogle Scholar
  33. Rygulla W, Snowdon RJ, Eynck C, Koopmann B, von Tiedemann A, Lühs W, Friedt W (2007) Broadening the genetic basis of Verticillium longisporum resistance in Brassica napus by interspecific hybridization. Phytopathology 97:1391–1396CrossRefPubMedGoogle Scholar
  34. Rygulla W, Snowdon RJ, Friedt W, Happstadius I, Cheung W, Chen D (2008) Identification of quantitative trait loci for resistance against Verticillium longisporum in oilseed rape (Brassica napus). Phytopathology 98:215–221CrossRefPubMedGoogle Scholar
  35. Silva HSA, Romeiro RS, Mounteer A (2003) Development of a root colonization bioassay for rapid screening of rhizobacteria for potential biocontrol agents. J Phytopathol 151:42–46CrossRefGoogle Scholar
  36. Srivastava LM (2007) Plant growth and development—hormones and environment. Academic Press, San DiegoGoogle Scholar
  37. Tjamos EC, Tsitsigiannis DI, Tjamos SE, Antoniou PP, Katinakis P (2004) Selection and screening of endorhizosphere bacteria from solarized soils as biocontrol agents against Verticillium dahliae of solanaceous hosts. Eur J Plant Pathol 110:35–44CrossRefGoogle Scholar
  38. Whipps J (2001) Microbial interactions and biocontrol in the rhizosphere. J Exp Bot 52:487–511PubMedGoogle Scholar
  39. Zeise K (1992) Screening for resistance to Verticillium dahliae Kleb. on oilseed rape (Brassica napus var. oleifera Metzger) under greenhouse conditions. Nachr Deut Pflanzenschutzd 44:125–128Google Scholar
  40. Zeise K, von Tiedemann A (2001) Morphological and physiological differentiation among vegetative compatibility groups of Verticillium dahliae and V. longisporum. J Phytopathol 149:469–475CrossRefGoogle Scholar
  41. Zeise K, von Tiedemann A (2002) Host specialization among vegetative compatibility groups of Verticillium dahliae in relation to Verticillium longisporum. J Phytopathol 150:112–119CrossRefGoogle Scholar
  42. Zhou L, Hu Q, Johansson A, Dixelius C (2006) Verticillium longisporum and V. dahliae: infection and disease in Brassica napus. Plant Pathol 55:137–144CrossRefGoogle Scholar

Copyright information

© International Organization for Biological Control (IOBC) 2010

Authors and Affiliations

  1. 1.Department of Biotechnology and Biological Control, Institute for PhytopathologyChristian Albrechts UniversityKielGermany

Personalised recommendations