BioControl

, Volume 52, Issue 6, pp 801–819

Occurrence and characterization of a nucleopolyhedrovirus from Maruca vitrata (Lepidoptera, Pyralidae) isolated in Taiwan

  • Song-Tay Lee
  • R. Srinivasan
  • Yan-Jheng Wu
  • N. S. Talekar
Article

Abstract

A nucleopolyhedrovirus (MaviMNPV) was isolated from diseased larvae of legume pod borer (LPB), Maruca vitrata, at Tainan in Taiwan. Electron microscopical studies on the ultrastructure of MaviMNPV occlusion bodies (OBs) showed several virions (up to 19) with multiple nucleocapsids (up to 6) packaged within a single viral envelope. The diameter of OBs was 0.9 to 1.3 µm with a mean of 1.152±0.116 µm. The complete sequence of the MaviMNPV polyhedrin (Polh) gene contained 735 nucleotides (GenBank accession number DQ399596). Phylogenetic analyses using the complete sequence of the Polh gene of MaviMNPV indicated that this virus clusters with Group I NPVs. The genome size of MaviMNPV estimated with restriction enzymes viz., HindIII, EcoRI, BglII and PstI was 113.41 ± 1.50 kbp. First instar LPB larvae were the most susceptible stage (LC50 2.053 × 102 OBs/ml) followed by second, third and fourth instars with the median lethal concentrations (LC50s) 1.410 × 103, 2.390 × 103 and 2.636 × 103 OBs/ml, respectively. This is the first record of this virus from this region.

Keywords

Lepidoptera Maruca vitrata Maruca vitrata multiple nucleopolyhedrovirus Polymerase Chain Reaction Pyralidae Restriction Enzyme Analysis Transmission Electron Microscopy 

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Copyright information

© International Organization for Biological Control (IOBC) 2007

Authors and Affiliations

  • Song-Tay Lee
    • 1
  • R. Srinivasan
    • 2
  • Yan-Jheng Wu
    • 1
  • N. S. Talekar
    • 2
    • 3
  1. 1.Department of BiotechnologySouthern Taiwan University of TechnologyYung-Kang CityTaiwan
  2. 2.AVRDC-The World Vegetable CenterTainanTaiwan
  3. 3.College of Plant ProtectionYunnan Agricultural UniversityKunmingChina

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