BioControl

, Volume 52, Issue 5, pp 657–667 | Cite as

Relative activity of baculoviruses of the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae)

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Abstract

The relative activities of the granulovirus (PxGV) and the nucleopolyhedrovirus (PxMNPV) of the diamondback moth, Plutella xylostella (L.), against this insect were evaluated in the laboratory. Similar numbers of occlusion bodies (OBs) of PxGV and PxMNPV were required to kill P. xylostella. However, cadavers of insects infected with PxGV contained more OBs per unit weight than did cadavers infected with PxMNPV. Less cadaver material was thus required to kill insects with PxGV. Larvae that were killed by PxGV survived approximately 2 d longer than did those killed by PxMNPV. When P. xylostella larvae were fed both viruses together, mortality was higher than that caused by either virus alone. The virulence of PxMNPV produced in P.␣xylostella did not differ from that of PxMNPV produced in the beet armyworm, Spodoptera exigua (Hübner). S. exigua was less susceptible to PxMNPV than to its homologous nucleopolyhedrovirus, SeMNPV.

Keywords

Granulovirus Lepidoptera Nucleopolyhedrovirus Plutella xylostella Spodoptera exigua 

Abbreviations

AcMNPV

Autographa californica multiple nucleopolyhedrovirus

AfMNPV

Anagrapha falcifera multiple nucleopolyhedrovirus

ANOVA

analysis of variance

ARS

Agricultural Research Service

GmMNPV

Galleria mellonella multiple nucleopolyhedrovirus

GV

granulovirus

LD50

median lethal dose

LSD

least significant difference

MNPV

multiple nucleopolyhedrovirus

OB

occlusion body

PxGV

Plutella xylostella granulovirus

PxMNPV

Plutella xylostella multiple nucleopolyhedrovirus

SeMNPV

Spodoptera exigua multiple nucleopolyhedrovirus

USDA

United States Department of Agriculture.

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Notes

Acknowledgements

We thank A. H. McIntosh (USDA-ARS, Columbia, MO, USA) for supplying the original sample of PxMNPV, L. J. Liska (USDA-ARS, Beltsville, MD, USA) for supplying P. xylostella larvae, R. Myers (USDA-ARS, Tifton, GA, USA) for supplying S. exigua eggs, C.␣Murphy (USDA-ARS, Beltsville, MD, USA) for electron microscopy of PxGV, and L. Lacey (USDA-ARS, Wapato, WA, USA) and D. Lynn (USDA-ARS, Beltsville, MD, USA) for comments on the manuscript.

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Copyright information

© International Organization for Biological Control (IOBC) 2007

Authors and Affiliations

  • Robert R. Farrar
    • 1
  • Martin Shapiro
    • 2
  • Merle Shepard
    • 2
  1. 1.Insect Biocontrol LaboratoryUSDA, Agricultural Research ServiceBeltsvilleUSA
  2. 2.Clemson UniversityCharlestonUSA

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