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Relative activity of baculoviruses of the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae)

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Abstract

The relative activities of the granulovirus (PxGV) and the nucleopolyhedrovirus (PxMNPV) of the diamondback moth, Plutella xylostella (L.), against this insect were evaluated in the laboratory. Similar numbers of occlusion bodies (OBs) of PxGV and PxMNPV were required to kill P. xylostella. However, cadavers of insects infected with PxGV contained more OBs per unit weight than did cadavers infected with PxMNPV. Less cadaver material was thus required to kill insects with PxGV. Larvae that were killed by PxGV survived approximately 2 d longer than did those killed by PxMNPV. When P. xylostella larvae were fed both viruses together, mortality was higher than that caused by either virus alone. The virulence of PxMNPV produced in P.␣xylostella did not differ from that of PxMNPV produced in the beet armyworm, Spodoptera exigua (Hübner). S. exigua was less susceptible to PxMNPV than to its homologous nucleopolyhedrovirus, SeMNPV.

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Abbreviations

AcMNPV:

Autographa californica multiple nucleopolyhedrovirus

AfMNPV:

Anagrapha falcifera multiple nucleopolyhedrovirus

ANOVA:

analysis of variance

ARS:

Agricultural Research Service

GmMNPV:

Galleria mellonella multiple nucleopolyhedrovirus

GV:

granulovirus

LD50 :

median lethal dose

LSD:

least significant difference

MNPV:

multiple nucleopolyhedrovirus

OB:

occlusion body

PxGV:

Plutella xylostella granulovirus

PxMNPV:

Plutella xylostella multiple nucleopolyhedrovirus

SeMNPV:

Spodoptera exigua multiple nucleopolyhedrovirus

USDA:

United States Department of Agriculture.

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Acknowledgements

We thank A. H. McIntosh (USDA-ARS, Columbia, MO, USA) for supplying the original sample of PxMNPV, L. J. Liska (USDA-ARS, Beltsville, MD, USA) for supplying P. xylostella larvae, R. Myers (USDA-ARS, Tifton, GA, USA) for supplying S. exigua eggs, C.␣Murphy (USDA-ARS, Beltsville, MD, USA) for electron microscopy of PxGV, and L. Lacey (USDA-ARS, Wapato, WA, USA) and D. Lynn (USDA-ARS, Beltsville, MD, USA) for comments on the manuscript.

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Authors and Affiliations

  1. Insect Biocontrol Laboratory, USDA, Agricultural Research Service, Bldg. 011A, BARC-West, Beltsville, MD, 20705, USA

    Robert R. Farrar

  2. Clemson University, Charleston, SC, 29414, USA

    Martin Shapiro & Merle Shepard

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  1. Robert R. Farrar
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  2. Martin Shapiro
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Correspondence to Robert R. Farrar.

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Use of trade names does not imply endorsements by USDA-ARS of products named nor criticism of similar ones not mentioned.

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Farrar, R.R., Shapiro, M. & Shepard, M. Relative activity of baculoviruses of the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae). BioControl 52, 657–667 (2007). https://doi.org/10.1007/s10526-006-9048-y

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