, Volume 15, Issue 2, pp 165–176 | Cite as

Cell proliferation arrest and redox state status as part of different stages during senescence establishment in mouse fibroblasts

  • Francisco Triana-Martínez
  • Norma E. López-Diazguerrero
  • Luis A. Maciel-Barón
  • Sandra L. Morales-Rosales
  • Sonia Galván-Arzate
  • Francisco J. Fernandez-Perrino
  • Alejandro Zentella
  • Viviana I. Pérez
  • Luis E. Gomez-Quiroz
  • Mina Königsberg
Research Article


Senescence phenotype can be achieved by multiple pathways. Most of them involve the activation of negative cell cycle regulators as well as a shift to an oxidative status. However, the exact participation of these events in senescence establishment and maintenance is not completely understood. In this study we investigated the content of three final cell cycle regulators, as well as the redox state in some critical points during the pre-senescent and the full-senescent states. Our results highlight the existence of a critical pre-phase in senescent phenotype establishment, in which cell proliferation stops with the participation of the cell cycle inhibitors, and a second maintenance stage where the exacerbated pro-oxidant state inside the cell induces the physiological decline characteristic in senescent cells.


Senescence P16 P21 P27 Oxidative stress Cell cycle 



The authors would like to thank Dr. A. Hernández from CINVESTAV for generously donating us the actin antibody. Dr. Rocío González-Vieira from UAM-I for animal supply. This work was supported by CONACyT’s grants CB-2006-1-59659 and CB-2012-1-178349. As well as the “Red Temática de Investigación en Salud y Desarrollo Social” from CONACYT. Triana-Martínez and Maciel-Barón are CONACYT scholarship holders.


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Francisco Triana-Martínez
    • 1
    • 2
  • Norma E. López-Diazguerrero
    • 1
  • Luis A. Maciel-Barón
    • 1
    • 2
  • Sandra L. Morales-Rosales
    • 1
  • Sonia Galván-Arzate
    • 3
  • Francisco J. Fernandez-Perrino
    • 4
  • Alejandro Zentella
    • 5
    • 6
  • Viviana I. Pérez
    • 7
  • Luis E. Gomez-Quiroz
    • 1
  • Mina Königsberg
    • 1
  1. 1.Departamento de Ciencias de la Salud, División de Ciencias Biológicas y de la SaludUniversidad Autónoma Metropolitana-IztapalapaMexicoMexico
  2. 2.Posgrado en Biologia ExperimentalMexico CityMexico
  3. 3.Dept. NeuroquímicaInstituto Nacional de Neurología y NeurocirugíaMexicoMexico
  4. 4.Dept. Biotecnología, DCBSUniversidad Autónoma Metropolitana IztapalapaMexicoMexico
  5. 5.Dept. Medicina Genómica y Toxicología Ambiental. IIBUNAMMexicoMexico
  6. 6.Depto BioquímicaINCMNSZMexicoMexico
  7. 7.Department of Biochemistry & BiophysicsOregon State UniversityCorvallisUSA

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