Biogerontology

, Volume 11, Issue 2, pp 183–195

Caenorhabditiselegans lifespan extension caused by treatment with an orally active ROS-generator is dependent on DAF-16 and SIR-2.1

  • Tanja Heidler
  • Kai Hartwig
  • Hannelore Daniel
  • Uwe Wenzel
Research Article

Abstract

In Caenorhabditiselegans pretreatment with juglone, a generator of reactive oxygen species (ROS) provides a subsequently increased ROS-resistance. We investigated whether juglone at low or high concentrations when provided via the oral route in a liquid axenic medium affects normal lifespan of C.elegans. High juglone concentrations led to premature death, low concentrations were tolerated well and caused a prolongation of lifespan. Lifespan extension under moderate oxidative stress was associated with increased expression of small heat-shock protein HSP-16.2, enhanced glutathione levels, and nuclear translocation of DAF-16. Silencing or deletion of DAF-16 prevented the juglone-induced adaptations. RNA-interference for SIR-2.1 had the same effects as the deletion of DAF-16 but did not affect nuclear accumulation of DAF-16. Our studies demonstrate that DAF-16- and SIR-2.1-dependent alterations in gene expression after a ROS challenge lead to a lifespan extension in C.elegans as long as the stressor concentration does not exceed the saturable protective capacity.

Keywords

Caenorhabditiselegans Hormesis Reactive oxygen species Stress response Small heat-shock proteins Glutathione 

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Tanja Heidler
    • 1
  • Kai Hartwig
    • 1
  • Hannelore Daniel
    • 1
  • Uwe Wenzel
    • 2
  1. 1.Department of Food and Nutrition, Molecular Nutrition UnitTechnical University of MunichFreisingGermany
  2. 2.Molecular Nutrition Research, Interdisciplinary Research CenterJustus-Liebig-University of GiessenGiessenGermany

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