Nonclassical export pathway: overexpression of NCE102 reduces protein and DNA damage and prolongs lifespan in an SGS1 deficient Saccharomyces cerevisiae
- 96 Downloads
In this study, we used our recently developed screening method, Bud-Scar-based Screening (BSS), to screen a yeast cDNA expression library in an SGS1 deletion BY4742 yeast strain. One gene involved in a nonclassical export pathway, NCE102, was found to extend the life span of Δsgs1 yeast. Deletion of NCE102 in a wild type yeast strain increased its sensitivity to oxidative stress upon diethylmaleate (DEM) treatment but did not shorten its lifespan, indicating that this gene is not essential in determining yeast lifespan. Transformation of NCE102 into either Δnce102 or Δsgs1 strains could rescue its tolerance to DEM stress, indicating that NCE102 is protective during oxidative stress. Moreover, overexpression of NCE102 in Δsgs1 strain leads to reduced protein damage. However, overexpression of NCE102 in wild type yeast strain BY4742 neither protected against oxidative stress due to DEM nor extended yeast lifespan compared to its parental wild type strain, indicating that nonclassical export is redundant and DNA repair is fully sufficient in the wild type strain. We therefore demonstrate that a nonclassical export pathway functions as an alternative clearance/detoxification pathway to eliminate damaged material, when the basic repair pathway is not sufficient.
KeywordsAging NCE102 SGS1 Budding yeast Nonclassical export pathway Oxidative stress Lifespan
- Heeren G, Jarolim S, Laun P, Rinnerthaler M, Stolze K, Perrone GG, Kohlwein SD, Nohl H, Dawes IW, Breitenbach M (2004) The role of respiration, reactive oxygen species and oxidative stress in mother cell-specific ageing of yeast strains defective in the RAS signalling pathway. FEMS Yeast Res 5:157–167PubMedCrossRefGoogle Scholar
- Pachler K, Karl T, Kolmann K, Mehlmer N, Eder M, Loeffler M, Oender K, Hochleitner EO, Lottspeich F, Bresgen N, Richter K, Breitenbach M, Koller L (2004) Functional interaction in establishment of ribosomal integrity between small subunit protein rpS6 and translational regulator rpL10/Grc5p. FEMS Yeast Res 5:271–280PubMedCrossRefGoogle Scholar
- Schaus SE, Cavalieri D, Myers AG (2001) Gene transcription analysis of Saccharomyces cerevisiae exposed to neocarzinostatin protein-chromophore complex reveals evidence of DNA damage, a potential mechanism of resistance, and consequences of prolonged exposure. Proc Natl Acad Sci USA 98:11075–11080PubMedCrossRefGoogle Scholar