Advertisement

Biogerontology

, Volume 8, Issue 3, pp 327–344 | Cite as

Hormetic effects on longevity of hydrogen peroxide in Drosophila melanogaster flies living on a poorly nutritious medium

  • Éric Le Bourg
Research Article

Abstract

Subjecting flies to a mild stress at a young age may increase longevity and protect against strong stresses occurring at middle age. The purpose of this article is to test whether a mild stress could also increase survival time of flies living in stressful conditions. Flies were transferred at middle age in vials where they could only feed on a saccharose solution without any other nutrient. This poor medium is known to decrease longevity and it was hypothetized that adding hydrogen peroxide to it could minimize this negative effect. While high doses of hydrogen peroxide decreased further longevity, a low dose increased it in 4-week-old males and, only in some experiments, in females. This low dose had however not any positive effect on behavioral aging, resistance to heat and starvation. The positive effect of hydrogen peroxide appeared not to be due to a sanitary action upon the environment. Rather, it seems that hydrogen peroxide was a mild stress helping flies to cope with the negative effects of saccharose on longevity. Therefore, it is concluded that hydrogen peroxide, beyond the deleterious effects of high doses, could have positive effects in organisms when used at a low dose, particularly in stressful living conditions.

Keywords

Drosophila melanogaster Behavioral aging Longevity Hydrogen peroxide Heat stress Starvation Mild stress Hormesis 

References

  1. Brummel T, Ching A, Seroude L, Simon AF, Benzer S (2004) Drosophila lifespan enhancement by exogenous bacteria. Proc Natl Acad Sci USA 101:12974–12979PubMedCrossRefGoogle Scholar
  2. Calabrese EJ (2006) The failure of dose–response models to predict low dose effects: a major challenge for biomedical, toxicological and aging research. Biogerontology 7:119–122PubMedCrossRefGoogle Scholar
  3. Calabrese EJ, Baldwin LA (2001) Hormesis: a generalizable and unifying hypothesis. Crit Rev Toxicol 31:353–421PubMedCrossRefGoogle Scholar
  4. Carvalho GB, Kapahi P, Benzer S (2005) Compensatory ingestion upon dietary restriction in Drosophila melanogaster. Nat Methods 2:813–815PubMedCrossRefGoogle Scholar
  5. Courgeon AM, Rollet E, Becker J, Maisonhaute C, Best-Belpomme M (1988) Hydrogen peroxide (H2O2) induces actin and some heat-shock proteins in Drosophila cells. Eur J Biochem 171:163–170PubMedCrossRefGoogle Scholar
  6. Cypser JR, Johnson TE (2002) Multiple stressors in Caenorhabditis elegans induce stress hormesis and extended longevity. J Gerontol Biol Sci 57A:B109–B114Google Scholar
  7. Driver CJI, Wallis R, Cosopodiotis G (1986) Is a fat metabolite the major diet dependent accelerator of aging? Exp Gerontol 21:497–507PubMedCrossRefGoogle Scholar
  8. Edgecomb RS, Harth CE, Schneiderman AM (1994) Regulation of feeding behavior in adult Drosophila melanogaster varies with feeding regime and nutritional state. J Exp Biol 197:215–235PubMedGoogle Scholar
  9. Feany MB, Bender WW (2000) A Drosophila model of Parkinson’s disease. Nature 404:394–398PubMedCrossRefGoogle Scholar
  10. Harman D (1956) Aging. A theory based on free radical and radiation chemistry. J Gerontol 11:298–300PubMedGoogle Scholar
  11. Hercus MJ, Loeschcke V, Rattan SIS (2003) Lifespan extension of Drosophila melanogaster through hormesis by repeated mild heat stress. Biogerontology 4:149–156PubMedCrossRefGoogle Scholar
  12. Hollingsworth MJ, Burcombe JV (1970) The nutritional requirements for longevity in Drosophila. J Insect Physiol 16:1017–1025PubMedCrossRefGoogle Scholar
  13. Kircher HW, Al-Azawi B (1985) Longevity of seven species of cactophilic Drosophila and D. melanogaster on carbohydrates. J Insect Physiol 31:165–169CrossRefGoogle Scholar
  14. Le Bourg E (2003) Delaying aging: could the study of hormesis be more helpful than that of the genetic pathway used to survive starvation? Biogerontology 4:319–324PubMedCrossRefGoogle Scholar
  15. Le Bourg E (2004) Effects of aging on learned suppression of photopositive tendencies in Drosophila melanogaster. Neurobiol Aging 25:1241–1252PubMedCrossRefGoogle Scholar
  16. Le Bourg E (2005) Hormetic protection of Drosophila melanogaster middle-aged male flies from heat stress by mildly stressing them at young age. Naturwissenschaften 92:293–296PubMedCrossRefGoogle Scholar
  17. Le Bourg E, Buecher C (2002) Learned suppression of photopositive tendencies in Drosophila melanogaster. Anim Learn Behav 30:330–341PubMedGoogle Scholar
  18. Le Bourg E, Minois N (1996) Failure to confirm increased longevity in Drosophila melanogaster flies submitted to a food restriction procedure. J Gerontol Biol Sci 51A:B280–B283Google Scholar
  19. Le Bourg E, Minois N (1997) Increased longevity and resistance to heat shock in Drosophila melanogaster flies exposed to hypergravity. C R Acad Sci Paris 320:215–221PubMedGoogle Scholar
  20. Le Bourg E, Minois N (1999) A mild stress, hypergravity exposure, postpones behavioral aging in Drosophila melanogaster. Exp Gerontol 34:157–172PubMedCrossRefGoogle Scholar
  21. Le Bourg E, Minois N, Bullens P, Baret P (2000) A mild stress due to hypergravity exposure at young age increases longevity in Drosophila melanogaster males. Biogerontology 1:145–155PubMedCrossRefGoogle Scholar
  22. Le Bourg E, Valenti P, Lucchetta P, Payre F (2001) Effects of mild heat shocks at young age on aging and longevity in Drosophila melanogaster. Biogerontology 2:155–164PubMedCrossRefGoogle Scholar
  23. Le Bourg E, Valenti P, Payre F (2002) Lack of hypergravity-associated longevity extension in Drosophila melanogaster files overexpressing hsp70. Biogerontology 3:355–364PubMedCrossRefGoogle Scholar
  24. Le Bourg E, Toffin E, Massé A (2004) Male Drosophila melanogaster flies exposed to hypergravity at young age are protected against a non-lethal heat shock at middle age but not against behavioral impairments due to this shock. Biogerontology 5:431–443PubMedCrossRefGoogle Scholar
  25. Min KJ, Tatar M (2006) Drosophila diet restriction in practice: do flies consume fewer nutrients? Mech Ageing Dev 127:93–96PubMedCrossRefGoogle Scholar
  26. Minois N, Rattan SIS (2003) Hormesis in aging and longevity. In: Rattan SIS (ed) Modulating aging and longevity. Kluwer Academic Publishers, Dordrecht, pp 127–137Google Scholar
  27. Miquel J, Lundgren PR, Binnard R (1972) Negative geotaxis and mating behavior in control and gamma-irradiated Drosophila. Drosoph Inf Serv 48:60–61Google Scholar
  28. Mockett RJ, Bayne ACV, Kwong LK, Orr WC, Sohal RS (2003) Ectopic expression of catalase in Drosophila mitochondria increases stress resistance but not longevity. Free Radic Biol Med 34:207–217PubMedCrossRefGoogle Scholar
  29. Pearl R, Allen A, Penniman WBD (1926) Culture media for Drosophila. II. A new synthetic medium and its influence on fertility at different densities of population. Am Nat 60:357–366CrossRefGoogle Scholar
  30. Tapia PC (2006) Sublethal mitochondrial stress with an attendant stoichiometric augmentation of reactive oxygen species may precipitate many of the beneficial alterations in cellular physiology produces by caloric restriction, intermittent fasting, exercise and dietary phytonutrients: “mitohormesis” for health and vitality. Med Hypotheses 66:832–843PubMedCrossRefGoogle Scholar
  31. Thompson ED, Reeder BA, Bruce RD (1991) Characterization of a method for quantitating food consumption for mutation assays in Drosophila. Environ Mol Mutagen 18:14–21PubMedCrossRefGoogle Scholar
  32. Vaiserman AM, Koshel NM, Litoshenko AY, Mozzhukina TG, Voitenko VP (2003) Effects of X-irradiation in early ontogenesis on the longevity and amount of the S1 nuclease-sensitive DNA sites in adult Drosophila melanogaster. Biogerontology 4:9–14PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  1. 1.Centre de recherche sur la cognition animaleUniversité Paul-SabatierToulouse cedex 9France

Personalised recommendations