Biogerontology

, Volume 8, Issue 3, pp 269–282

Expression of decorin and collagens I and III in different layers of human skin in vivo: a laser capture microdissection study

  • Katrin Lochner
  • Astrid Gaemlich
  • Kirstin Marie Südel
  • Kirsten Venzke
  • Ingrid Moll
  • Anja Knott
  • Franz Stäb
  • Horst Wenck
  • Olaf Döring
  • Michael Böttger
  • Stefan Gallinat
Original Research

DOI: 10.1007/s10522-006-9070-6

Cite this article as:
Lochner, K., Gaemlich, A., Südel, K.M. et al. Biogerontology (2007) 8: 269. doi:10.1007/s10522-006-9070-6

Abstract

Extracellular matrix (ECM) organization is a complex process that requires the coordinated efforts of many molecules. For the regulation of collagen fiber diameter, the proteoglycan decorin appears to be of major relevance. To investigate the role of decorin in the process of (photo-)aging in more detail, full-thickness punch biopsies were isolated from human buttock skin. Single exposure with two minimal erythemal doses of solar simulated irradiation caused down-regulation of decorin mRNA in young (n = 5) and old subjects (n = 5) after 24 h. Interestingly, decorin mRNA was elevated with age. To test the hypothesis that a decreased collagen-to-decorin-ratio impairs collagen structure we also investigated collagens I and III gene expression. Both were down-regulated with increasing age and after single UV-irradiation. As determined by laser capture microdissection-quantitative real time-Polymerase chain reaction (n = 11), decorin is mostly present in the reticular dermis while being absent from the papillary dermis. Minor expression was also observed in the epidermis. However, in contrast to full-thickness skin biopsies age-dependent changes in collagens I, III, and decorin expression could not be observed with this methodology indicating technical limitations. Together with our finding that collagens I and III mRNA are similarly expressed in the reticular and papillary dermis and are down-regulated by UV, our studies support the idea of a major role of decorin in ECM organization. Altered expression of decorin mRNA in the different dermal strata and a decrease in the collagen-to-decorin ratio inflicted by both age and ultraviolet irradiation possibly affect collagen bundle diameter and subsequently the mechanical properties of human skin.

Keywords

Collagen Decorin Extracellular matrix Human Photoaging 

Abbreviations

BSA

Bovine serum albumin

Col Ia1

Collagen Ia1

Col IIIa1

Collagen IIIa1

cT

Threshold cycle

DAPI

4′-6-Diamidino-2-phenylindole

DEPC

Diethyl Pyrocarbonate

dIdC

2′-deoxyinosinic-2′-deoxycytidylic acid

DNA

Desoxyribonucleic acid

cDNA

Complementary Desoxyribonucleic acid

DCN

Decorin

E

Epidermis

ECM

Extracellular matrix

HE

Hematoxylin eosin staining

LCM

Laser capture microdissection

MED

Minimal erythema dose

nm

nano meter

PBS

Phosphate buffered saline

PD

Papillary dermis

PET

Polyethylen-Terephthalat

RD

Reticular dermis

RT-PCR

Real time-Polymerase chain reaction

SP

Subpapillary plexus

SSR

Solar simulated radiation

UV

Ultra violet

UVA

Ultra violet A

RNA

Ribonucleic acid

aRNA

antisense Ribonucleic acid

mRNA

messenger Ribonucleic acid

rRNA

ribosomal Ribonucleic acid

μm

micro meter

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Katrin Lochner
    • 1
  • Astrid Gaemlich
    • 1
  • Kirstin Marie Südel
    • 1
  • Kirsten Venzke
    • 1
  • Ingrid Moll
    • 2
  • Anja Knott
    • 1
  • Franz Stäb
    • 1
  • Horst Wenck
    • 1
  • Olaf Döring
    • 3
  • Michael Böttger
    • 3
  • Stefan Gallinat
    • 1
  1. 1.Paul Gerson Unna Skin Research CenterBeiersdorf AGHamburgGermany
  2. 2.Clinic and Polyclinic for Dermatology and VenerologyUniversity Hospital Hamburg-EppendorfHamburgGermany
  3. 3.Department of Plant PhysiologyUniversity of HamburgHamburgGermany

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