Biogerontology

, Volume 7, Issue 5–6, pp 449–459 | Cite as

Simultaneous evaluation of circulating chemokine and cytokine profiles in elderly subjects by multiplex technology: relationship with zinc status

  • Erminia Mariani
  • Luca Cattini
  • Simona Neri
  • Marco Malavolta
  • Eugenio Mocchegiani
  • Giovanni Ravaglia
  • Andrea Facchini
Research Article

Abstract

Cellular components of both adaptive and innate immune systems produce different chemokines and cytokines, involved in different signalling pathways among cells, and modulate effector function during immune response, playing a key role in the regulation of the type and extent of the immune response in the elderly. We evaluated the circulating concentration of selected chemokines: MCP-1, MIP-1α, IL-8, RANTES together with IL-6 and TNF-α in plasma obtained from a group of healthy old subjects, in order to highlight possible differences in the synthesis of these factors, assuming that both the cytokine and the chemokine networks are remodelled with ageing. The simultaneous evaluation was performed by a multiplex analysis system. In addition, since micronutrient deficiency may underlie an inflammatory response, the association between chemokine levels and a nutritional element such as zinc was also evaluated, since the immune system is the first system to be affected by changing zinc levels, due to its high cell turnover. A progressive age-related increase of plasma concentrations of all soluble factors was observed. The increment was particularly evident for IL-6, IL-8, MCP-1 and TNF-α in the over 85-year-old group in concomitance with increasing percentages of subjects with low circulating levels of zinc. In conclusion, the remodelling of chemokine profiles, skewed to Th2 response by both advanced ages and circulating levels of zinc, might reflect different states of activation and/or responsiveness of the human immune cell/mediator network, thus influencing the ability to develop rapid innate and long-lasting adaptive immune responses with advancing age.

Keywords

Chemokine profiles Inflammation Ageing Zinc Multiplex immunoassay Bio-Plex™ 

Notes

Acknowledgements

This work was partially supported by grants from Bologna University (60% fund), Ricerca Corrente IOR, Italian Health Ministry fund, and was performed under the aegis of the EU ZINCAGE project (FOOD-CT-2003-506850). The authors thank Mrs Patrizia Rappini and Graziella Salmi for typing assistance.

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Erminia Mariani
    • 1
    • 2
  • Luca Cattini
    • 1
  • Simona Neri
    • 1
  • Marco Malavolta
    • 3
  • Eugenio Mocchegiani
    • 3
  • Giovanni Ravaglia
    • 4
  • Andrea Facchini
    • 1
    • 2
  1. 1.Laboratorio di Immunologia e GeneticaIstituto di Ricerca Codivilla-Putti, IOR, Via di Barbiano 1/10BolognaItaly
  2. 2.Dipartimento di Medicina Interna e GastroenterologiaUniversity of BolognaBolognaItaly
  3. 3.Immunology Center (Nutrition, Immunity and Ageing Section)INRCAAnconaItaly
  4. 4.Dipartimento di Medicina Interna Cardioangiologia ed EpatologiaUniversity of BolognaBolognaItaly

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