, 8:97 | Cite as

Supplementation with a complex of active nutrients improved dermal and epidermal characteristics in skin equivalents generated from fibroblasts from young or aged donors

  • Sophie Lacroix
  • Charbel Bouez
  • Sandrine Vidal
  • Valérie Cenizo
  • Corinne Reymermier
  • Virginie Justin
  • Jana Vičanová
  • Odile Damour
Research Article


Cultured skin equivalent (SE, Mimeskin®) was generated by co-culturing skin fibroblasts and keratinocytes on a collagen-glycosaminoglycan-chitosan dermal substrate. In order to examine donor age effect, fibroblasts from 19- (young) or 49- (aged) year-old females were used. Culture medium was supplemented with nutrients complex containing soy extract, tomato extract, grape seed extract, white tea extract, sodium ascorbate, tocopherol acetate, zinc gluconate and BioMarine™ complex. Epidermal and dermal structure and composition were examined after 42 and 60 days of culture. In untreated samples, SE generated from young fibroblasts was superior to SE from aged fibroblasts in all characteristics. Those include number and regularity of keratinocyte layers, number of keratinocytes expressing proliferation marker Ki67, content of collagen type I, fibrillin-1, elastin, and SE lifespan. Effects of nutritional supplementation were observed in SE from both young and aged fibroblasts, however, those effects were more pronounced in SE from aged fibroblasts. In epidermis, the treatment increased number of keratinocyte layers and delayed epidermal senescence. The number of cells expressing Ki67 was nine folds higher than those of controls, and was similar to that of young cell SE. In dermis, the treatment increased mRNA synthesis of collagen I, fibrillin-1 and elastin. In conclusion, skin cell donor age had major important effect on formation of reconstructed SE. Imperfections in epidermal and dermal structure and composition as well as life span in SE from aged cells can be improved by supplementation with active nutrients.


Ageing Dermis Fibroblast Imedeen In vitro Mimeskin Keratinocyte Skin equivalent 



Extracellular matrix


Epidermal growth factor


Dulbecco’s modified Eagle’s medium




Complex of active ingredients from Imedeen Prime Renewal™


Reverse transcription polymerase chain reaction


Skin equivalent


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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Sophie Lacroix
    • 1
  • Charbel Bouez
    • 1
  • Sandrine Vidal
    • 1
  • Valérie Cenizo
    • 2
  • Corinne Reymermier
    • 3
  • Virginie Justin
    • 1
  • Jana Vičanová
    • 4
    • 5
  • Odile Damour
    • 1
    • 2
  1. 1.Banque de Tissus et Cellules, Hôpital Edouard HerriotHospices Civils de LyonLyonFrance
  2. 2.Institut de Biologie et Chimie des ProtéinesLyonFrance
  3. 3.EngelhardLyonFrance
  4. 4.DermDataTrutnovCzech Republic
  5. 5.UnhostCzech Republic

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