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Biogerontology

, Volume 5, Issue 6, pp 375–381 | Cite as

Estrogen receptor α expression in mice kidney shows sex differences during aging

  • P. K. Sharma
  • M. K. ThakurEmail author
Research article

Abstract

We have used semi quantitative RT-PCR and western blotting for the analysis of expression of estrogen receptor (ER) α and βmRNA and protein in the kidney of adult and old mice of both sexes. Uterus, a well-known target for estrogen action, was used for comparison. As compared to adult, the expression of both ER α mRNA and protein of old mice decreased in male but increased in female. However, unlike uterus, neither ERβ mRNA nor protein could be detected in the kidney of adult or old mice. Thus the present data reveal that the expression of ERα in mice kidney shows sex differences during aging.

Keywords

aging estrogen receptor kidney mice semi quantitative RT-PCR western blot 

Abbreviations

βME

βmercaptoethanol

ECL

enhanced chemiluminescence

EDTA

ethylenediaminetetraacetic acid

ER

Estrogen receptor

PAGE

polyacrylamide gel electrophoresis

PBS

phosphate buffered saline

PCR

polymerase chain reaction

PMSF

phenyl methyl sulphonyl fluoride

PVDF

polyvinyl difluoride

RT

reverse transcriptase

SDS

sodium dodecyl sulphate

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  1. 1.Biochemistry and Molecular Biology Laboratory, Center of Advanced Study in ZoologyBanaras Hindu UniversityVaranasiIndia

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