, Volume 5, Issue 6, pp 375–381 | Cite as

Estrogen receptor α expression in mice kidney shows sex differences during aging

  • P. K. Sharma
  • M. K. ThakurEmail author
Research article


We have used semi quantitative RT-PCR and western blotting for the analysis of expression of estrogen receptor (ER) α and βmRNA and protein in the kidney of adult and old mice of both sexes. Uterus, a well-known target for estrogen action, was used for comparison. As compared to adult, the expression of both ER α mRNA and protein of old mice decreased in male but increased in female. However, unlike uterus, neither ERβ mRNA nor protein could be detected in the kidney of adult or old mice. Thus the present data reveal that the expression of ERα in mice kidney shows sex differences during aging.


aging estrogen receptor kidney mice semi quantitative RT-PCR western blot 





enhanced chemiluminescence


ethylenediaminetetraacetic acid


Estrogen receptor


polyacrylamide gel electrophoresis


phosphate buffered saline


polymerase chain reaction


phenyl methyl sulphonyl fluoride


polyvinyl difluoride


reverse transcriptase


sodium dodecyl sulphate


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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  1. 1.Biochemistry and Molecular Biology Laboratory, Center of Advanced Study in ZoologyBanaras Hindu UniversityVaranasiIndia

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