Effects of Repeated Treatment with Midazolam in SHR and SLA16 Rat Strains in the Triple Test
We exposed male and female rats of SHR (Spontaneously Hypertensive Rats) and SLA16 (SHR.LEW-Anxrr16) strains, in a non-drugged state, for five consecutive days to the Triple Test (experiment 1); or after repeated treatment with midazolam (MDZ), for four consecutive days. The fifth day was performed without treatment (experiment 2). The first experiment showed that males did not avoid and females increased the exploration of the open arms over the days. In experiment 2, SLA16 from both sexes approached more the open arms than SHR rats. The MDZ anxiolytic-like effect was sustained in both strains and sexes over the days. On the fifth day, SLA16 still approached more the open arms than SHR rats. Data suggest an absence of repeated-trial tolerance to MDZ anxiolytic-like effects. Testing the SHR and SLA16 strains, especially females, could be necessary for the future search for the genes and molecular pathways underlying anxiety/emotionality.
KeywordsMidazolam Anxiety Emotionality Anxiolytic drugs Rats
F. J. Correa and R. C. N. Marchette were recipients of fellowships from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). L. R. Acuña; N. GranzottoF. G. Mazur and E. Corvino were recipients of fellowships from Coordenadoria de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). The authors also thank R. A. N. Pértile and T. M. Alves for their technical assistance.
This work was supported by Edital MCTI/CNPq 14/2013.
Compliance with ethical standards
Conflict of interest
Lucía Raily Acuña, Renata Cristina Nunes Marchette, Natalli Granzotto, Paula Gomes Dias, Maria Elisa Corvino, Fernando Gabriel Mazur, Fernanda Junques Corrêa and Geison Souza Izídio declare that they have no conflict of interest.
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were by following the ethical standards of the institution or practice at which the studies were conducted and respected the guidelines of the local committee for Animal Care in Research (CEUA/UFSC) with the valid permission PP00903.
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