Behavior Genetics

, Volume 43, Issue 2, pp 168–176 | Cite as

Comparison of Whole-Genome DNA Methylation Patterns in Whole Blood, Saliva, and Lymphoblastoid Cell Lines

  • Tara M. Thompson
  • Duaa Sharfi
  • Maria Lee
  • Carolyn M. Yrigollen
  • Oksana Yu Naumova
  • Elena L. Grigorenko
Brief Communication

Abstract

Epigenetic mechanisms, including DNA methylation, that underlie neuropsychiatric conditions have become a promising area of research. Most commonly used DNA sources in such studies are peripheral (whole) blood (WB), saliva (SL), and lymphoblastoid cell lines (LCLs); thus, the question of the consistency of DNA methylation patterns in those cells is of particular interest. To investigate this question we performed comparative analyses of methylation patterns in WB, SL, and LCLs derived from the same individuals, using Illumina HumanMethylation27 BeadChip arrays. Our results showed that DNA methylation patterns in SL are relatively consistent with those in WB, whereas the patterns in LCLs are similarly distinct from both WB and SL. The results indicated that due to multiple random and directed changes in DNA methylation throughout cell culturing, LCLs are not a reliable source of DNA for epigenetic studies and should be used with caution when investigating epigenetic mechanisms underlying biological processes.

Keywords

Lymphoblastoid cell lines Saliva Whole blood DNA methylation Methylation pattern 

Supplementary material

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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Tara M. Thompson
    • 1
  • Duaa Sharfi
    • 1
    • 2
  • Maria Lee
    • 1
  • Carolyn M. Yrigollen
    • 1
    • 3
  • Oksana Yu Naumova
    • 1
    • 4
  • Elena L. Grigorenko
    • 1
    • 5
    • 6
    • 7
  1. 1.Yale UniversityNew HavenUSA
  2. 2.University of IllinoisChicagoUSA
  3. 3.University of CaliforniaDavisUSA
  4. 4.Vavilov Institute of General Genetics of the Russian Academy of SciencesMoscowRussian Federation
  5. 5.Moscow State University for Psychology and EducationMoscowRussian Federation
  6. 6.Columbia UniversityNew YorkUSA
  7. 7.Child Study Center, Department of Psychology, Department of Epidemiology and Public HealthYale UniversityNew HavenUSA

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