Genetic Mapping of Vocalization to a Series of Increasing Acute Footshocks Using B6.A Consomic and B6.D2 Congenic Mouse Strains
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Footshock response is used to study a variety of biological functions in mammals including drug self-administration, learning and memory and nociception. However, the genetics underlying variability in footshock sensitivity are not well understood. In the current studies, a panel of B6.A consomic mouse strains, two B6.D2 genome-tagged mouse lines, and the progenitor strains were screened for footshock sensitivity as measured by audible vocalization. It was found that A/J (A) mice and C57BL/6J (B6) mice with an A Chromosome 1 (Chr 1) were less sensitive to footshock compared to B6 animals. Furthermore, the offspring of Chr 1 consomic mice crossed with B6 mice had vocalization levels that were intermediate to A/J and B6 animals. A F2 mapping panel revealed two significant QTLs for footshock vocalization centered around D1Mit490 and D1Mit206 on Chr 1. The role of these Chr 1 loci in footshock sensitivity was confirmed in B6.D2 genome-tagged mouse lines.
KeywordsStress Footshock Vocalization Mice QTL Chromosome 1
This manuscript was supported by the following grants: NIMH grant MH61971, R25 MH-066890, U01-AA-13503, AA014588, AA13509, DA020677, and AA016662 to the authors.
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