Bulletin of Experimental Biology and Medicine

, Volume 168, Issue 3, pp 371–374 | Cite as

Evaluation of the Efficiency of Lubricant Based on Pulmonary Surfactant in Experimental Knee Osteoarthritis in Rats: Analysis of 3D Reconstructions

  • P. A. KrylovEmail author
  • A. S. Astakhov
  • E. N. Nesmeyanova
  • N. A. Borozdina
  • V. V. Novochadov

We evaluated the efficiency of a lubricant based on a pulmonary surfactant in experimental knee osteoarthritis in rats induced by intraarticular injection of abrasive material that reduces the lubricative properties of the synovial fluid. Experimental substance containing proteins of the pulmonary surfactant exhibiting natural lubricative properties was used as the lubricant. The effectiveness of the substance was analyzed by changes in morphological characteristics of the articular cartilage in 3D reconstruction images of the knee joint obtained by the method of multiple high-precision grinding. It was found that radial thickness of the articular cartilage increased and surface relief index decreased on the 6th and 12th week after administration of the substance containing surfactant proteins, which can indicate partial recovery of the articular cartilage.

Key Words

articular cartilage experimental osteoarthritis surfactant-associated proteins 3D reconstruction the knee joint rat 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Kotelnikov GP, Lartsev YuV, Makhova AN. Comparative evaluation of structural changes of joint tissues in various models of experimental arthrosis. Kazan. Med. Zh. 2006;87(1):31-35. Russian.Google Scholar
  2. 2.
    Malanin DA, Novochadov VV, Demkin SA, Demeshenko MV, Danilov DI. Autologous platelet-rich plasma in the treatment of patients with knee arthritis III stage. Travmatol. Ortoped. Rossii. 2014;(3):52-59. Russian.CrossRefGoogle Scholar
  3. 3.
    Povoroznyuk VV, Orlik TV, Kozitskaya SV. Innovative drug “Gialgel” for the treatment of patients with grade II-III joint osteoarthritis. Poliklinika. 2016;(1-1):72-73. Russian.Google Scholar
  4. 4.
    Terpilovskiy AA, Tiras KP, Khoperskov AV, Novochadov VV. The possibilities of full-color three-dimensional reconstruction of biological objects by the method of layer-by-layer overlapping: knee joint of a rat. Vestn. Voldograd. Gos. Univer. Ser. 11. Estestv. Nauki. 2015;(4):6-14. Russian.Google Scholar
  5. 5.
    Agrawal V, Smart K, Jilling T, Hirsch E. Hirsch Surfactant protein (SP)-A suppresses preterm delivery and inflammation via TLR2. PLoS One. 2013;8(5). ID e63990. doi: CrossRefGoogle Scholar
  6. 6.
    Freitag J, Bates D, Boyd R, Shah K, Barnard A, Huguenin L, Tenen A. Mesenchymal stem cell therapy in the treatment of osteoarthritis: reparative pathways, safety and efficacy — a review. BMC Musculoskelet. Disord. 2016;17. ID 230. doi:
  7. 7.
    Kraus VB, Huebner JL, DeGroot J, Bendele A. The OARSI histopathology initiative — recommendations for histological assessments of osteoarthritis in the Guinea Pig. Osteoarthritis Cartilage. 2010;18(Suppl. 3):S35-S52.CrossRefGoogle Scholar
  8. 8.
    Maradit Kremers H, Larson DR, Crowson CS, Kremers WK, Washington RE, Steiner CA, Jiranek WA, Berry DJ. Prevalence of Total Hip and Knee Replacement in the United States. J. Bone Joint Surg. Am. 2015;97(17):1386-1397.CrossRefGoogle Scholar
  9. 9.
    McAlindon TE, LaValley MP, Harvey WF, Price LL, Driban JB, Zhang M, Ward RJ. Effect of intra-articular triamcinolone vs saline on knee cartilage volume and pain in patients with knee osteoarthritis. JAMA. 2017;317(19):1967-1975.CrossRefGoogle Scholar
  10. 10.
    Novochadov VV, Krylov PA. Production technology and physicochemical properties of composition containing surfactant proteins. Eur. J. Mol. Biotechnol. 2016;(2):77-84.Google Scholar
  11. 11.
    Rivera F, Bertignone L, Grandi G, Camisassa R, Comaschi G, Trentini D, Zanone M, Teppex G, Vasario G, Fortina G. Effectiveness of intra-articular injections of sodium hyaluronate-chondroitin sulfate in knee osteoarthritis: a multicenter prospective study. J. Orthop. Traumatol. 2016;17(1):27-33.CrossRefGoogle Scholar
  12. 12.
    Sarker M, Jackman D, Booth V. Lung surfactant protein A (SP-A) interactions with model lung surfactant lipids and an SP-B fragment. Biochemistry. 2011;50(22):4867-4876.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • P. A. Krylov
    • 1
    Email author
  • A. S. Astakhov
    • 1
  • E. N. Nesmeyanova
    • 1
  • N. A. Borozdina
    • 1
  • V. V. Novochadov
    • 1
  1. 1.Department and Bioengineering and BioinformaticsVolgograd State UniversityVolgogradRussia

Personalised recommendations